Thermo Scientific 3403 User manual

Midi 40 CO2 Incubator
Models 3403 and 3404
Service Manual 7993403 Rev. 2
_________________________________________________________________________________
Part of Thermo Scientific

Thermo Scientific
Manual Number 7993403
2 28728 6/18/12 Added full CO2 calibration procedure - Section 7 ccs
1 27807 10/3/11 Updated with op manual revisions ccs
0 -- 12/10/09 Original, was 97665H08 ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Midi 40 CO2 Incubator i

Thermo Scientificii Midi 40 CO2 Incubator
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
Progressive product changes will be entered into the service manual as it is revised. The manual is intended as an
aid to persons qualified in the service of applicable equipment. It is not intended to teach unqualified persons
on applicable equipment all procedures necessary to make repairs.
©2009 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific Midi 40 CO2 Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo products will be available at www.thermo.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Midi 40 CO2 Incubator
Preface
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technicians or a qualified service organization for on-the-spot repair. If your service need is
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Midi 40 CO2 Incubator vThermo Scientific
Table of Contents
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Included Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Wiring Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
CO2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Model and Serial Number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Component Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Technical Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Cabinet Construction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Microprocessor Control Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Features and Benefits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Alarm Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Control Systems Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Door Heat System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Overtemperature Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . .4-1
CO2 Control Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Temperature Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Preventive Maintenance and Diagnostics . . . . . . . . . . . . . . . . . . . . . . .5-1
Recommended Tool List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Troubleshooting Parameter List . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Section 1
Section 2
Section 3
Section 4
Section 5

vi Midi 40 CO2 Incubator Thermo Scientific
Maintenance & Repair Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Replacing the Gas Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Replacing the Temperature Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Replacing CO2 Sensor and Interface PCB . . . . . . . . . . . . . . . . . . . . .6-3
Replacing Printed Circuit Boards . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4
Testing the Outer Door Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Replacing the Outer Door Heater . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6
Replacing CO2 Solenoid Assembly . . . . . . . . . . . . . . . . . . . . . . . . . .6-7
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Access Program or Service Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
CO2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3
Appendices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
IntrLogic Power/Key On Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
IntrLogic Test Points and Jumpers . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Common Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
How to Enter Test Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
IntrLogic: Board Test Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3
Telemetry Tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-5
Detailed Descriptions of IntrLogic PCBs . . . . . . . . . . . . . . . . . . . . .8-10
Relay and Triac Allocation on Power PCB . . . . . . . . . . . . . . . . . .8-11
General Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-13
Interface PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-20
CO2 Sensor PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-24
Electrical Schematic and Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-2
Table of Contents
Section 6
Section 8
Section 7
Section 9

Midi 40 CO2 Incubator 1-1Thermo Scientific
Section 1 General Information
The purpose of this manual is to provide procedures developed to assist
the field refrigeration technician in troubleshooting and repairing certain
conditions.
The incubators described in this manual are classified for use as stationary
equipment in a Pollution Degree 2 and Overvoltage Category II
environment, according to the UL3101-1 and IEC 664 standards.
These units are designed to operate under the following environmental
conditions:
• Indoor use
• Altitude up to 2000m
• Maximum relative humidity 80% for temperatures up to 31°C
• Main supply voltage fluctuations not to exceed 10% of the nominal
voltage.
Symphony incubators are designed to create a stable, reliable environment
for cell culture applications. They operate at temperatures ranging from
5°C above ambient temperature to +40°C, accurate to ±0.2°C. The gas
system controls within ±0.1% of measurable setpoint.
Each chamber consists of two sections: the cabinet (where the product is
stored) and the control section (containing the temperature, CO2, and
humidity control systems). On single-chamber models, the control section
is to the side of the cabinet.
Table 1: Typical Dimensions
Height Width Front to Back
18 in. 23.5 in. 18.5 in.
46.7 cm 59.7 cm 47 cm

1-2 Midi 40 CO2 Incubator Thermo Scientific
Section 1
General Information
Locate the unit in a level area free from vibration with a minimum of
three inches (7.6 cm) of space on the sides and rear and 12 inches (30.5
cm) at the top. The table must be able to support 40 PSI. Also, allow
enough clearance so that the door can swing open at least 90 degrees, and
the power cord can easily be reached to disconnect power. Do not
position the equipment in direct sunlight or near any HVAC
duct/diffusers. The ambient temperature range at the location must be
59°F to 90°F (15° to 32°C).
The following items are packaged and shipped inside the incubator
cabinet:
• The operating manual
• Shelves and shelf brackets
• 1/4 in. (476 mm) ID clear tubing for the gas connection
• Cordset
• 5” round humidity pan
• Additional CO2sensor gasket
•CO
2disposable filter 99.97
Location
Included Parts
Figure 4-1. Included Components (shelves and brackets not shown)
Cordset
Additional
gasket
Humidity
pan
Tubing
Filter
Operating
manual

Midi 40 CO2 Incubator 1-3Thermo Scientific
Section 1
General Information
Verify that the incubator gas supply is available near the installation area.
The required gas supply pressure is 15 PSI, controlled by a high-quality,
two-stage regulator suitable for the connected input gas.
Do not connect the gas at this time.
Caution Connect the equipment to the correct power source. Be sure to
operate the incubator at the voltage specified on the dataplate. Incorrect
voltage can result in severe damage to the equipment. s
Warning Failure to properly ground the equipment may cause personal
injury or damage to the equipment. s
Caution Always conform to National Electrical Code and local codes. s
Warning Do not connect the unit to overloaded power lines. s
The incubators described in this manual are rated for supply voltage
115/120VAC, single-phase, 60Hz, 3 amps.
Always connect the unit to a dedicated (separate) circuit. Do not exceed
the electrical and temperature ratings printed on the data plate. Electrical
codes require fuse or circuit breaker protection for branch circuit
conductors.
Model numbers are important because they identify the unit for service
capability. The serial number is a unique number that identifies the unit
as a specific unit. No other unit has that same number.
The model and serial numbers of your incubator are printed on a plastic
identification plate, commonly referred to as the data plate. The most
important information concerning your unit can be gathered from the
model number and the serial number identification. Additional
information is available on the data plate; such as the voltage, frequency,
and current ratings.
The data plate is mounted on the outside of the cabinet, beside the upper
door hinge. Some older models may have the plate mounted on the front
of the frame, just above the lower door hinge.
Model and Serial
Number
CO2 Gas Supply
Wiring Cautions