Thermo Scientific 3010 Operating instructions

Model 3010
Water Jacketed CO2 Incubator
Operating and Maintenance Manual 7003010 Rev. 7

Thermo ScientificThermo Scientific
MANUAL NUMBER 7003010
7 25248/IN-3945 6/09/09 Updated shelf specifications - sliding and stationary ccs
6 25303/IN-3902 2/17/09 Added UL decom warning pg 1-5 ccs
5 25103/IN-3881 2/9/09 Updated expl parts dwg 3010-201-1-D to Rev. 3 ccs
4 24975 12/09/08 Updated water specs on page 1-6 ccs
3 23451/IN-3294 6/30/06 Added ±5 psig to incoming CO2 pressure range ccs
2 22511/PIP-115 11/12/04 Changed the chamber from 1900215 to 1900600 ccs
-- 22434/IN-3211 8/25/04 Updated water quality description for water jacket and humidity pan ccs
1 21730/SI-9181 6/16/04 Added alternate 250085 solenoid valve ccs
0 -- 8/11/03 Original ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Water Jacketed CO2 Incubator i

Thermo Scientificii Water Jacketed CO2 Incubator Thermo Scientific
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In
no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of
this manual.
©2003 Thermo Scientific. All rights reserved.

Thermo Scientific Water Jacketed CO2 Incubator iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo’s compliance with this directive, the recyclers in your country and information on Thermo
products will be available at www.thermofisher.com.
✔Always use the proper protective equipment (clothing, gloves, goggles, etc.)
✔Always dissipate extreme cold or heat and wear protective clothing.
✔Always follow good hygiene practices.
✔Each individual is responsible for his or her own safety.

Thermo Scientificiv Water Jacketed CO2 Incubator Thermo Scientific
Preface
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Thermo Scientific Water Jacketed CO2 Incubator vThermo Scientific
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•Locate the model and serial numbers. A serial tag is located on the unit itself.
• For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty
• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
a billable service at your location. Calibration after a warranty repair is covered under the warranty.
• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
• Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient temperature fluctuations
8) any repair external to the unit
• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
• Repairs to parts or systems resulting from unauthorized unit modifications.
• Any labor costs other than that specified during the parts and labor warranty period, which may
include additional warranty on CO2sensors, blower motors, water jackets, etc.

Water Jacketed CO2 Incubator viThermo Scientific
Table of Contents
Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Control Panel Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2
Unit Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Stack the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Access Port Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Connect Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Level the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Fill the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Fill the Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Connect the CO2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Set the Operating Temperature Setpoint . . . . . . . . . . . . . . . . . . . .1-10
Set the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Set the CO2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-12
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrate Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrate Thermal Conductivity CO2 System . . . . . . . . . . . . . . . . . .2-2
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Clean the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Reverse the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Reverse the Hinges for Exterior Door . . . . . . . . . . . . . . . . . . . . . . .4-4
Clean the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Clean the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Replace Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-10
Replace the Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
Replace the Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11
Add or Replenish Rust Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Drain Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12
Remote Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 8
Section 7
Section 9

Water Jacketed CO2 Incubator 1-1Thermo Scientific
Section 1 Installation and Start-Up
• Outer Door - Reversible to opposite swing, see Section 4.4
• Heated Inner Door - Keeps chamber interior dry. Reversible to opposite
swing, see Section 4.4.
• Chamber Gas Sample Port - Used for sampling chamber CO2 content,
using a Fyrite or similar instrument.
• Main Power Switch
• Control Panel - Keypad, Displays and Indicators. (See Figure 1-2).
• Fill Port - Used for filling the water jacket.
• Water Jacket Vent- Do not cover! Allows air to escape from the water
jacket during filling and normal expansion and contraction when the
incubator heats or cools.
• Leveling Legs - Used to level the unit.
• Water Jacket Drain – Remove plug and use hose barb insert included.
Plug when not in use.
Note The incubators are stackable. Information follows in this section. ▲
*When setting up the incubator, install the cover plate packed inside the incubator
shipping carton.
Heated Glass
Inner Door
Leveling
Legs (4)
Jacket
Vent
Water Jacket
Fill Port
Jacket
Drain
(remove plug before using)*
Control Panel
Chamber Gas
Sample Port
Power
Switch
Hose
Barb
Insert
OverTemp
LowTemp
HighCO2
LowCO2
AddWater
DoorOpen
MODE
SILENCE
Run
SetTemp
SetOtemp
SetCO2
CalTemp
CalCO2
ENTER
Inject
Heat Temp°C
%CO2
Figure 1-1. Water Jacket Incubator

1-2 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
• SILENCE - Silences the audible alarm.
• Alarm Indicators - Specific indicator flashes during an alarm condition.
• MODE Select Switch - Used to select desired mode.
• Mode Indicator - Selected mode indicator lights.
Mode Select Indicators-
Run: Normal Operation
Set Temp: Set Temperature Setpoint
Set Otemp: Set Overtemperature Setpoint
Set CO2: Set CO2 Control Setpoint
Cal Temp: Calibrate Displayed Temperature (Offset)
Cal CO2: Calibrate Displayed CO2 Value (Offset)
• Up and Down Arrows: Increase or decrease parameter values.
• Enter: Saves the value being changed.
• Heat Indicator: Lights when power is applied to the heaters.
• Inject Indicator: Lights when gas is being injected into the incubator.
• Displays: Displays chamber temperature, temperature setpoint or
overtemperature setpoint
• Displays: Displays percentage of CO2 in the chamber or CO2 setpoint
The Model 3010 water jacket incubator has six modes, which allow
incubator setup. The modes are as follows:
• Run is the default mode for the incubator during operation.
• Set Temp is used to enter system temperature setpoints for incubator
operation.
• Set Otemp is used to enter system overtemperature setpoints for incu-
bator operation.
• Set CO2 is used to enter system CO2 setpoints for incubator operation.
• Cal Temp is used to calibrate displayed chamber temperature.
• Cal CO2 is used to calibrate displayed percentage of CO2 in chamber.
Control Panel Keys,
Displays & Indicators
Over Temp
Low Temp
High CO2
Low CO2
Add Water
Door Open
MODE
SILENCE
Run
Set Temp
Set Otemp
Set CO2
Cal Temp
Cal CO2
ENTER
Inject
Heat Temp °C
% CO2
Alarm indicators Mode indicators
Up and down
arrow keys
Heat
indicator
Inject
indicator
Displays chamber temp,
temp setpoint, overtemp setpoint
Displays CO2% in chamber,
CO2 setpoint
Figure 1-2. Control Panel
Control Panel
Operation

Water Jacketed CO2 Incubator 1-3Thermo Scientific
Section 1
Installation and Start-Up
Stack the Incubators
1. Locate the unit on a firm, level surface capable of supporting the unit’s
operational weight of 365 lbs. (166kg).
2. Locate away from doors and windows and heating and air
conditioning ducts.
3. Allow enough clearance behind the unit for electrical and gas hook-up.
Warning If the units have been in service, disconnect the power cord
connector and drain the water jacket of the designated top unit before
stacking. ▲
Note Stacking brackets (shown at left), washers, and bolts
for stacking are included with each unit. ▲
Figure 1-3. Stacking brackets
1. Designate one incubator to be the top unit and the other as the
bottom unit. Remove the base cover plate from the top unit using the
finger holes in the base, or a slotted screwdriver (Figure 1-4).
2. Note the two slots in the base of the incubator that accommodate the
stacking bolts. Refer to Figure 1-5.
Unit Installation
Figure 1-4. Remove Base Cover Plate
Figure 1-5. Stacking Bolt Slots

3. Remove the two plastic plugs from the bolt holes in the exterior top of
the bottom unit. Install the 1/2” long 5/16-18 stacking bolts and
washers into the bolt holes - do not tighten the bolts at this time.
Refer to Figure 1-6.
4. Unscrew and remove the leveling feet from the top unit and lift it onto
the bottom unit, off-setting the base of the top unit approximately 2-3
inches behind the stacking bolts and washers.
Warning This incubator weighs 265 lbs (120kg) before filling. Have
sufficient personnel to lift it. ▲
5. Align the sides of the top unit with the bottom unit and slide the top
unit forward until the slots in the base of the top unit align with the
5/16”-18 stacking bolts in the exterior top of the bottom unit. Refer
to Figure 1-7.
1-4 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Stack Incubators
(continued)
Figure 1-6. Bolt Holes

6. Remove and save the two screws from the back of the control panel on
the bottom unit as shown in Figure 1-8.
7. Insert the stacking brackets into the slots on the rear of the control
panel of the bottom unit (Figure 1-5). Align the slots in the brackets
with the mounting holes on the rear of the incubators. Secure the
brackets with the screws saved above and the 1/4-20 bolts provided in
the stacking kit. A 7/16” wrench or socket will be required for the
bolts.
8. Secure the base of the top unit to the exterior top of the bottom unit
by tightening the 5/16-18 stacking bolts using a 1/2” (13mm) wrench
or suitable tool.
9. Replace the base cover on the top unit.
10. The stacked units are ready to be placed into service.
1. Remove the protective plastic coating on the shelf supports and air
duct, if present.
2. Using a suitable laboratory disinfectant, clean all interior surfaces
including shelves and shelf supports, door gaskets, blower wheel and
CO2 sensor. See Section 5.
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user. ▲
Water Jacketed CO2 Incubator 1-5Thermo Scientific
Section 1
Installation and Start-Up
Stack Incubators
(continued)
Figure 1-8. Remove and Save Screws
Preliminary Cleaning

Locate the opening in the top left corner of the interior chamber. Remove
the tape from the opening on the outside of the unit. Locate the stopper
with filter in the hardware bag. Install in the opening inside the chamber.
See Figure 1-9.
1. Remove the filter from the shipping bag.
2. Separate one section of the tubing from the filter. Install this section to
the fitting on the blower plate.
3. After installing the top duct, connect the filter assembly to the tubing
coming through the top duct.
4. Insert the free end of the air sample filter tubing into the larger hole in
the back of the blower scroll. See Figure 1-9 for completed
configuration.
1. Install the side ducts with the tabs facing
into the center of the chamber with their
slots up. There are no right side or left
side ducts, simply rotate one of them to
fit the opposite side. Tilt the side ducts as
they are placed in the chamber so the tops
fit into the top air duct, then guide them
into the vertical position. Figure 1-10
shows the duct as it would be oriented for
the right side of the chamber.
1-6 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Access Port
Installation
Temp
sensor
Access
port filter
Air sample
filter
Figure 1-9. Filters
Air Sample Filter
Installation
Shelf Installation
Figure 1-10. Channels

2. Referring to Figure 1-10, note that there is no difference between left
and right side shelf channels.
3. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the side duct. Pull the shelf channel forward
and engage the channel’s front slot into the side duct’s appropriate
forward tab. Refer to Figure 1-11.
4. Figure 1-12 shows one of the channels installed on the right side duct.
Check the unit for being level by placing a bubble-style level on one of the
shelves. Turn the hex nut on the leveler counterclockwise to lengthen the
leg, or clockwise to shorten it. Level the unit front-to-back and left-to-
right.
See the serial tag on the side of the unit for electrical specifications, or refer
to the electrical schematics included in this manual.
Warning Connect the incubator to a grounded dedicated circuit only. The
power cord connector is the mains disconnect device for the incubator.
Position the unit so that it can be easily disconnected. ▲
Plug the provided power cord into the power inlet connector (Figure 1-13)
and into the grounded dedicated circuit.
Water Jacketed CO2 Incubator 1-7Thermo Scientific
Section 1
Installation and Start-Up
Level the Unit
Shelf Installation
(continued)
Figure 1-11. Channel Tabs Figure 1-12. Channel Installed
Connect Unit to
Electrical Power
Figure 1-13. Rear Panel, Showing All Options

Turn the power switch on. ADD WATER will appear in the message
center. Press the Silence key to silence the alarm.
Note The fill port has a plug that must be removed before filling and
replaced after filling is complete. ▲
Caution Chlorine is detrimental to stainless steel. Using chlorinated tap
water, or additives that contain chlorine, will void the water-jacket
warranty! ▲
Fill the water jacket with 11.7 gallons (43.5 liters) of pure water. Silicone
tubing and a funnel are shipped in the accessory bag with the unit.
For ease of connecting/disconnecting, use the silicone
tubing provided to connect directly to the fill port. See
Figure 1-1 and this detail. Do not install vinyl tubing
directly onto the fill port as it may be difficult to
remove. A 3/8” to 3/8” hose connector has been
provided to attach lengths of vinyl tubing to the silicone fill port tubing, to
reach the distilled water source.
When the jacket is full, the audible alarm sounds a continuous tone for 10
seconds and the alarm condition clears. Refer to Section 4, Table of
Alarms.
Note Model 3010 Water Jacket Incubator is shipped from the factory with
a rust inhibitor added to the water inside the unit. The rust inhibitor must
be replenished every 2 years. See Section 5 to drain the water jacket and for
the correct proportion of rust inhibitor to the water. ▲
For best operation of the incubator, sterilized distilled, demineralized or de-
ionized water should be used in the humidity pan. Water purity should be
in the resistance range of 50K to 1M Ohm/cm, or a conductivity range of
20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88 for
measuring water purity.
Distillation systems, as well as some types of reverse osmosis water purity
systems, can produce water in the quality range specified. Tap water is not
recommended as it may contain chlorine, which can deteriorate the
stainless steel. Tap water may also have a high mineral content, which
would produce a build-up of scale in the pan. High purity or ultra pure
water is not recommended as it is an extremely aggressive solvent and will
deteriorate the stainless steel. High purity water has a resistance of above
1M to 18M Ohm.
1-8 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Fill the Water Jacket
Detail 1. Fill Port
Fill the Humidity Pan

Even high purity water can contain bacteria and organic contaminants.
Water should always be sterilized or treated with a decontaminant, safe for
use with stainless steel as well as safe for the product, prior to being
introduced into the humidity pan.
Caution Distilled or de-ionized water used in the humidity reservoir must
be within a water quality resistance range of 50K to 1M Ohm/cm to
protect and prolong the life of the stainless steel. Use of water outside the
specified range will decrease the operating life of the unit and void
warranty. ▲
Fill the humidity pan to within 1/2 inch of the top with sterile, distilled
water. Place the pan directly on the incubator floor to ensure optimum
humidity and temperature response.
For applications requiring higher humidity conditions, the pan should be
placed against the left side wall of the incubator. The ductwork has been
modified for this purpose. Also, the CO2 sample port may be capped to
assist in achieving greater RH. In some ambients, this may cause
condensation to form in the chamber.
Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will have
an adverse effect on the humidity level, as well as CO2 calibration.
Warning High concentrations of CO2 gas can cause asphyxiation! OSHA
Standards specify that employee exposure to carbon dioxide in any eight-
hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure
limit for 15 minutes or less is 30,000 PPM (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. ▲
The CO2 gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range and the low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa), ±5 psig.
The incubator has a serrated fitting on the back of the cabinet to connect
the gas supply. Refer to Figure 1-13. The fitting is labeled CO2 Inlet.
Make sure that the connection is secured with a clamp. Check fitting for
leaks.
Water Jacketed CO2 Incubator 1-9Thermo Scientific
Section 1
Installation and Start-Up
Fill the Humidity Pan
(continued)
Connect the CO2 Gas
Supply

Warning This incubator is designed to be operated with CO2 gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO2 should not be connected to this equipment. CO2
gas cylinders have UN1013 labeled on the cylinder and are equipped with
a CGA 320 outlet valve. Check the gas cylinder for the proper
identification labels. The CO2 gas supply being connected to the incubator
should be industrial grade, 99.5% pure. Do not use CO2 gas cylinders
equipped with siphon tubes. A siphon tube is used to extract liquid CO2
from the cylinder, which can damage the pressure regulator. Consult with
your gas supplier to ensure that the CO2 cylinder does not contain a
siphon tube. Gas cylinders should also be secured to a wall or other
stationary object to prevent them from tipping.
A two-stage CO2 pressure regulator is required to be installed on the outlet
valve of the gas cylinder. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa), ±5 psig, for proper performance of the
CO2 control system. (A single stage CO2 pressure regulator will not
maintain 15 psig.
If higher purity CO2 is desires inside the incubator (greater than 99.5%
pure), the pressure regulator should be constructed with a stainless steel
diaphragm along with specifying the purity of the CO2 from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder. Consult your
facility safety officer to ensure that the equipment is installed in accordance
with the codes and regulations that are applicable in your area. ▲
Now that the incubator has been properly installed, connected to power,
filled with water, humidity pan filled, and connected to gas supply, system
setpoints can be entered. The following setpoints can be entered:
temperature, over temperature, and CO2.
The temperature setpoint range is 10° to 55°C, settable to within 0.1°C.
The lowest temperature the incubator can control is +5°C above the
ambient temperature at the incubator’s location. This incubator is shipped
from the factory with a temperature setpoint of 10°C. At this setting, all
heaters are turned off.
1-10 Water Jacketed CO2 Incubator Thermo Scientific
Section 1
Installation and Start-Up
Connect the CO2 Gas
Supply (continued)
Incubator Start-Up
Set the Operating
Temperature Setpoint

To change the temperature setpoint:
1. Press the Mode key until the Set Temp indicator lights (see illustration
above).
2. Press the up/down arrow until the desired temperature setpoint is
displayed.
3. Press Enter to save the setpoint.
4. Press the Mode key until the Run Indicator lights to go to Run mode.
Caution The overtemp circuit is designed as a safety to protect the
incubator only. It is not intended to protect or limit the maximum
temperature of the cell cultures or customer’s equipment inside the
incubator if an overtemp condition occurs. ▲
The factory setting for the Overtemperature setpoint is 40°C. It can be set
over a range of temp setpoint +0.5°C to 60°C. If the temperature setpoint
is moved above the Overtemp setpoint, the Overtemp will automatically
update to 1.0°C + the temp setpoint. It is recommended that the
Overtemp setpoint be 1°C over the temp setpoint.
To set the Overtemp setpoint:
1. Press the Mode key until the Set Otemp indicator lights (see
illustration above).
2. Press the up/down arrow until the desired Overtemp setpoint is
displayed.
3. Press Enter to save the setpoint.
4. Press the Mode key until the Run Indicator lights to go to Run mode.
Water Jacketed CO2 Incubator 1-11Thermo Scientific
Section 1
Installation and Start-Up
Set the Overtemp Setpoint
Set the Operating Temp
Setpoint (continued)