Biotage TurboVap 96 Dual User manual

TurboVap® Dual Quick Start Guide | Page 
Patent Pending
© Biotage 2021
TurboVap® Dual Quick Start Guide
System Maintenance
» Do not touch the inside of the gas heater. Touching may
leave residues that can contaminate the following runs.
» Ensure that the screws are tightened by hand using the Torx
T20 key (P/N 411886).
1. Loosen the fume shield set screw and remove the fume
shield.
2. Replace the manifold.
Clean the Manifolds
Both methods require a well collection plate, 2 mL or larger, that
fits the manifold that is to be cleaned.
After the manifold is cleaned, ensure that the nozzles are dry
before initiating an evaporation run. Either wait until they
are dry, or do a run with an empty cabinet and unheated gas.
(25°C/77°F)
1. Fill the collection plate with an appropriate solvent. The
solvent is dictated by analyte solubility:
TX20 ×2
For instructions regarding Manual mode, Time mode, and
software settings see the quick start guide Set Up and Perform
a Run. For instructions regarding Method mode, see the quick
start guide Set up a Method. For detailed instructions, see the
TurboVap®96 Dual User Manual. Patent pending.
Instrument Overview
There are three different manifolds configurations available for
the system; 24, 48, and 96 gas nozzles. The well plate or rack
must be compatible with the installed manifold.
AA
BB
CC
DD
EE
FF
GG
HH
II
ATouch screen FLift
BLED lights GSensor
CGas heater HDoors
DManifold IFume shield set screw
EPlate heater
Replace the Manifolds
Warning
» The nozzles may have come into contact with harmful sample
residues. Avoid contact.
» Always turn off the system, unplug the power cord, and let the
system cool down before performing maintenance.

TurboVap® Dual Quick Start Guide | Page 
Patent Pending
© Biotage 2021
Part Number: 418005-B
© 2021 Biotage. All rights reserved. No material may be reproduced or published without the written permission of Biotage.
Information in this document is subject to change without notice and does not represent any commitment from Biotage. E&OE.
A list of all trademarks owned by Biotage AB is available at www.biotage.com/legal. Other product and company names mentioned
herein may be trademarks or registered trademarks and/or service marks of their respective owners, and are used only for
explanation and to the owners’ benefit, without intent to infringe.
Clean a Manifold in Place
1. Put the collection plate on the plate heater under the
manifold.
2. Close the door and use the user interface to lift the
collection plate so the nozzles are submerged in the solvent.
(Do not start a run.)
3. Keep the nozzles submerged in the solvent for 5-10 minutes.
Note: For polar analytes it is recommended to repeat step 1 to 4
with a high organic solvent combination after the high aq rinse.
4. Lower the collection plate and remove it.
Clean a Manifold Outside of the Instrument
1. Remove the manifold.
2. Put the collection plate in a sonicator.
Ensure that the water level is below the edge of the
container. Water in the container will lead to contamination
3. Carefully lower the Manifold into the container and sonicate
for 5-10 minutes.
4. Remove the assembly from the sonicator and either put the
manifold into storage or attach it to the instrument.
Troubleshooting
Too Slow
Evaporation
» Ensure that there is no leakage between
the manifold and the plate heater by
tightening the manifold screws using the
supplied Torx T20 key (P/N 411886).
» Ensure that the plate heater
temperature is not too low.
» Ensure that the gas flow is not too low.
» Ensure that the gas inlet flow is not
leaking.
Uneven
Evaporation
» Inspect the nozzles for occlusion or
debris.
» Ensure that there are no bent nozzles.
» Ensure that the manifold and the well
plate are aligned. If they are misaligned,
contact Biotage.
Too Low Recovery
of Samples
» Ensure that the plate heater
temperature is not too high.
» Ensure that the gas flow is not too high.
» Ensure that the sample is not left
too long on the plate heater after the
evaporation is completed.
Low or No Gas
Flow
» Ensure that there are no leaks.
» Ensure that the pressure meets the
minimum pressure requirement.
» Ensure that the provided inlet tube is
used. A longer inlet tube may cause the
pressure to drop.
Touch Screen Is
Not Responding as
Expected
» Calibrate the touch screen. See
Troubleshooting in the User Manual.