Biotage PRESSURE+ 96 User manual

413248-A
BIOTAGE SWEDEN AB US TECHNICAL SUPPORT EU AND GLOBAL TECHNICAL SUPPORT
Box 8, SE-751 03 Uppsala, Sweden Phone: [phone] press 3 Phone: [phone]
Visiting Address: Vimpelgatan 5 US-1-pointsu[email protected] EU-1-p[email protected]om
Switchboard: [phone]
[email protected], www.biotage.com
Biotage PRESSURE+96
Positive Pressure Manifold
User Manual

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Contents
Safety....................................................................................................3
Intended Use ........................................................................................3
Warranty ..............................................................................................3
Service.................................................................................................3
Equipment Safety Standards ...................................................................3
Labels ..................................................................................................3
Safety Considerations.............................................................................4
Installation............................................................................................5
Site Requirements..................................................................................5
Gas Source ........................................................................................5
Unpack and Install .................................................................................5
Packing List........................................................................................7
System Description................................................................................8
Load the Plates......................................................................................8
Flow Control..........................................................................................9
Operation ............................................................................................ 10
Step-by-Step Instructions ..................................................................... 10
Maintenance ........................................................................................ 13
Replace the Column Seal....................................................................... 13
Clean ................................................................................................. 13
General Information ............................................................................ 14
Trademarks.........................................................................................14
Copyright............................................................................................ 14
Manufacturer....................................................................................... 14
Contact Us.......................................................................................... 14
Sales Offices and Distributors .............................................................14
Technical Support ............................................................................. 15

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Safety
Intended Use
Biotage PRESSURE+ 96 is intended for laboratory use only and has to be
operated by trained professionals. All operations must be performed:
According to the user documentation delivered with the equipment.
According to instructions available at www.biotage.com.
According to instructions given by the technical support staff from
Biotage.
Within limits set by the technical specification.
Failure to follow these instructions and operate within the limits set by the
technical specification may result in personal injury and/or equipment damage.
Warranty
PRESSURE+ 96 is warranted for 90 days against defects in materials and
workmanship under normal operating conditions and proper maintenance (see
page 13).
Service
All service or adjustments shall be performed by an authorized Biotage service
engineer. Before handing over the unit for service, it should be cleaned from
harmful residues as described in the Clean section on page 13.
Equipment Safety Standards
PRESSURE+ 96 and its instruction materials are certified by Biotage to comply
with:
CE-Marketing Directive 93/68/EEC
Machinery Directive 2006/42/EC
Labels
Labels used on PRESSURE+ 96:
In accordance with all the essential requirements of all applicable
European product directives; see “Equipment Safety Standards”
above.
Consult accompanying user documentation.
Manufacturer.

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Safety Considerations
Many reagents and samples used with PRESSURE+ 96 can pose chemical,
biological and radiological hazards. You must always understand the potentially
hazardous effects of all the materials you work with. To prevent personal injury
and/or equipment damage, we recommend that you always use impermeable
gloves, chemical safety goggles and protective clothing, follow GLP (Good
Laboratory Practice), and consult your company’s safety expert for guidance.
PRESSURE+ 96 units are manufactured from aluminum, stainless steel and
plastics. Always clean up spills or overflow immediately.

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Installation
Site Requirements
The PRESSURE+ 96 unit should be placed in a laboratory and operated where
there is proper ventilation or from inside a chemical fume hood when volatile
solvents are used. The PRESSURE+ 96 unit requires a compressed gas supply,
and should be located with this in mind.
Table/bench: Flat, stable, and able to support the weight of the
instrument (11.8 kg).
The dimensions of the unit is 271 x 305 x 325 mm (W x D
x H). The free space above and behind the bench should
be at least 10 cm for ventilation.
Operating
temperature: 5°C to 40°C, indoor use only
Humidity: Maximum relative humidity of 80% for temperatures up to
31°C, decreasing linearly to 50% relative humidity at 40°
Altitude: Up to 2000 meters
Gas Source
PRESSURE+ 96 operates using a compressed gas both to seal the columns and
displace liquid. Whether compressed air or nitrogen is used, or cylinders of
purified air or nitrogen, the gas should be free of moisture, particulates, and
hydrocarbons. This is essential to prevent sample contamination and general
fouling of the manifold.
There are a variety of filters available for the removal of moisture, particulates,
and hydrocarbons most commonly found within house gas supply systems. The
optimum gas supply (80 psi) is achieved by a regulator positioned between the
gas source and the unit. The supplied gas supply installation kit provides the
parts necessary to make the connection between the gas regulator and the unit.
Minimum pressure: 60 psi (4.1 bar)
Maximum pressure: 100 psi (6.9 bar)
Optimum pressure: 80 psi (5.5 bar)
Unpack and Install
WARNING
The total weight of the package including the unit is 16 kg . Follow
regional safety practices when handling, moving, and unpacking shipping
boxes and containers.

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Keep hands, loose clothing, and long hair away from the manifold when
connecting the gas supply. Slight movement may occur as the unit
pressurizes.
NOTE: Biotage recommend that the boxes and packing materials are retained by
the customer in case the unit needs to be transported or stored.

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The unit will already have the tubing attached in the back as shown in the left
image below.
1. Open the inner box and remove the smaller boxes. These contain the gas
supply installation kit, column seal, etc; check the contents against the
packing list below.
2. Gently pull the unit from the box and set the unit on a bench or in a fume
hood. See site requirements on page 5.
3. Using the supplied gas supply installation kit, plumb the back of the unit
to the gas supply (see images below). Refer to the Gas Source section on
page 5 for gas requirements.
4. Position the unit so that the handle and gauges are facing forward and the
gas supply is in the rear. Note that the unit weighs 11.8 kg.
Connect the gas line to the right port at the back of the unit.
Packing List
PRESSURE+ 96 is shipped with the following items:
Quantity Description Part Number
1 1 mL x 96 collection plate 121-5202
1 2 mL x 96 collection plate 121-5203
1 10 mL x 24 collection plate (waste plate) PPM-A96-1024
1 96-column seal, extra
(contains a hex key and 4 positioning
screws)
PPM-A96-GSKT
1 Gas supply installation kit
(6' of 1/8" i.d. polyethylene tubing and
1/8" and 1/4" NPT connectors)
PPM-GA
1 User Manual CD PPM-Man

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System Description
PRESSURE+ 96 is a positive pressure manifold designed to process 96-well
extraction plates using a pressurized gas source.
Load the Plates
PRESSURE+ 96 is compatible with all Biotage 96-well plate designs, including
fixed-well plates and modular arrays. The extraction plates should sit snugly on
top of the collection plate. Note that collection plates should allow for venting of
the positive pressure. Standard 1 mL and 2 mL collection plates from Biotage
will allow venting when paired with any of the 96-well formats from Biotage.
IMPORTANT: Other manufacturer’s collection plates may be used, however,
users must ensure that the assembly of extraction plate and collection plate can
vent when positive pressure is applied.
The handgrip on the front of the platform helps you to slide the platform with the
extraction and collection plates forward and backward under the manifold. Stack
the extraction plate onto the collection plate and set the stack onto the sliding
platform.
To lower the manifold, push the platform to the back of the track until it reaches
the stop point (under the upper manifold), then press down on the two rocker
switches on the sides of the unit simultaneously and hold for approximately 3
seconds. This will activate the upper manifold and it will self-adjust to any plate
assembly height by lowering and compressing. If you let go too soon, the upper
manifold will rise up and you can simply repeat by pressing down on the rocker
switches and holding them again for approximately 3 seconds.
To release the plates, press up on both rocker switches, and then slide the plate
assembly forward.
NOTE: The upper manifold is powered by the gas source and the speed is pre-
set and not adjustable.
IMPORTANT: An important safety feature of the unit is the requirement to hold
both rocker switches for approximately 3 seconds simultaneously to lower the
manifold. This is to keep hands clear of moving parts during compression.
Lower the manifold by pressing down on the two rocker switches simultaneously
for approximately 3 seconds.

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Flow Control
The PRESSURE+ 96 unit has a two-tier gas delivery system. The flow control
switch (B) enables setting and operation of gas flow in addition to turning gas
flow on and off:
Adjustable Flow Rate (0 to 15 psi, 0 to 2.5 scfh)
Recommended for sample loading and elution. By turning the flow control switch
(B) to the left to the Adjustable Flow position, gas is channeled through the
Adjustable Flow regulator (C) and then through a rotometer (A) controlled by the
needle valve positioned at its base. This path through the rotometer provides
very precise and slow flow through the columns by limiting gas flow from 0 to 2.5
scfh (standard cubic feet per hour).
IMPORTANT: As indicated on the label, never turn the rotometer completely off.
Seating the needle valve into its orifice can damage the unit.
Maximum Flow Rate (0 to 100 psi)
Recommended for column washing, drying, etc. By turning the flow control switch
(B) to the right to the Maximum Flow position, gas is introduced at the pressure
set on the Maximum Flow regulator. The pressure range for this regulator is 0 to
100 psi and should be used when processing very viscous or dirty samples.
Turn Off the Gas
By turning the flow control switch (B) to the OFF position, no gas will flow
through the manifold.
IMPORTANT: The unit should always be in the OFF position during compression
and decompression cycles and when not in use. Always shut off the external gas
supply when the unit is not in use.
NOTE: In case of troubleshooting, for each well, with no column in place, the
flow at 25 psi should be 0.75 ±0.05 scfh (standard cubic feet per hour) of flow
through each well.

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Operation
WARNING
Keep hands, loose clothing, and long hair away from the manifold when it is
moving.
To avoid spillage, ensure to stack the plates properly on the platform.
To prevent personal injury and/or equipment damage, we recommend that you
always use impermeable gloves, chemical safety goggles and protective clothing,
follow GLP (Good Laboratory Practice), and consult your company’s safety expert
for guidance.
Step-by-Step Instructions
1. Ensure the unit is connected to a gas supply; see Installation section on page 5.
2. Place a 96-well collection plate on the platform.
3. Place a 96-well extraction plate on top of the collection plate.
4. Load each well with conditioning solvent.

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5. Slide the platform back to position the 96-well extraction plate
under the manifold.
6. Turn the flow control switch to the left to the Adjustable Flow position.
7. Adjust the regulator and rotometer to achieve the desired flow rate.
8. Lower the manifold by pressing down on the two rocker switches simultaneously
for approximately 3 seconds.

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9. When liquid has finished flowing, turn the flow control switch to the
OFF position.
10.Raise the pressure manifold by pressing up on the two rocker switches
simultaneously.
11.Pull out the platform.
12.Repeat step 4 through 11 as required for sample application and wash steps.
13.Slide the platform back to position the 96-well extraction plate under the
manifold.
14.Turn the flow control switch to the right to the Maximum Flow position and adjust
the manifold pressure to the desired setting for the drying period.
15.Lower the manifold by pressing down on the two rocker switches simultaneously
for approximately 3 seconds.
16.After the sorbent bed is dry, turn the flow control switch to the OFF position
17.Raise the manifold by pressing up on the two rocker switches simultaneously.
18.Pull out the platform and replace the solvent reservoir with a collection plate.
19.Add elution solvent to each well.
20.Allow samples to elute by gravity or repeat step 5 through 11 for pressure
assisted elution. Tip: To trigger gravity elution, slide the platform back to position
the plate under the manifold, ensure the flow control switch is in the OFF position
and then lower the manifold. Raise the manifold after a few seconds.

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Maintenance
Replace the Column Seal
You need a 96-column seal, a hex key and four positioning screws (part number
PPM-A96-GSKT), and a Philips screwdriver (PH2). In order to maintain
compliance, column seals supplied by Biotage must be used with the unit.
1. Shut off the external gas supply.
2. Turn the unit’s flow control switch to the right to the Maximum Flow
position.
3. When there is no remaining pressure in the unit (0 psi), turn the unit’s
flow control switch to the OFF position.
4. Remove the two 1/4-20 hex screws that hold the upper manifold to the
unit (standoffs) using the hex key.
5. Lift the manifold off the standoffs and place it upside down on the bench-
top.
6. Remove the old column seal from the manifold and clean the manifold
using ethanol.
7. Attach the four positioning screws to the threaded holes on each of the
corners of the manifold using a Philips screwdriver (PH2).
8. Remove the backing of the replacement column seal.
9. Align the holes on the new column seal with the manifold’s using the
positioning screws and adhere the seal.
10.Once the column seal is in place, remove the four positioning screws.
11.Fasten the manifold (with the column seal facing down) to the standoffs
using the two hex screws and the hex key.
NOTE: The adhesive on the column seals is volatile and cannot be stored for long
periods of time at room temperature. For extended storage, place column seals
in refrigerated conditions at 2°C to 5°C.
Clean
Materials of construction are primarily stainless steel and anodized aluminum.
Other materials include silicone rubber, polyethylene, and polypropylene. Always
clean up spills or overflow immediately.
If you wish to clean the column seal, this can be done by using a soft
cloth, dampened with methanol. The column seal is manufactured from
silicone rubber.
To clean the manifold, use a soft cloth, dampened with water. Never spray
or apply water directly to the manifold. Eye and hand protection is
recommended.
NOTE: The controls and gauges are not solvent resistant.

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General Information
Trademarks
The following trademarks are owned by Biotage AB: Biotage, Advancer,
AFFINILUTE, Endeavor, EVOLUTE, ExploraSep, Firefly design ( ), FLASH+,
FlashMaster, FlashVac, HPFC, HP-SIL, Initiator, Isolera, ISOLUTE, IST, IST
design ( ), Kiloprep, KP-C18-HS, KP-C18-WP, KP-C4-WP, KP-NH, KP-Sil,
MIP4SPE, MIP Rule of 6, MIP[4]Process, MIP[4]Proteins, PathFinder, PRESSURE+
48, PRESSURE+ 96, RapidTrace, Resolux, Samplet, SIM, SNAP, SP1, SP4, Syro
Wave, TurboVap, VacMaster, V-10, ZIF, ZIF-SIM, ZIP, and 1-Point Support.
Other product and company names mentioned herein may be trademarks or
registered trademarks and/or service marks of their respective owners, and are
used only for explanation and to the owners' benefit, without intent to infringe.
Copyright
Information in this document is subject to change without notice and does not
represent any commitment from Biotage Sweden AB.
All contents © 2011 Biotage Sweden AB. All rights reserved. No material may be
reproduced or published without the written permission of Biotage Sweden AB.
Contact Us
Biotage AB
Box 8
SE-751 03 Uppsala
Sweden
Visiting Address: Vimpelgatan 5
Phone: [phone]
Fax: [phone]
E-mail: [email protected]m
Website: www.biotage.com
Sales Offices and Distributors
Please visit www.biotage.com for contact information.

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Technical Support
Call, fax, or e-mail Biotage 1-Point Support, or request support online
(www.biotage.com). When contacting 1-Point Support, please have the following
information ready:
Company name and contact information.
Serial number of your PRESSURE+ 96 (see the product label at the rear of
the unit).
A brief description of the symptoms or technical problems you are
experiencing.
1-Point Support USA
Phone: [phone]
Outside US: [phone]
Fax: [phone]
E-mail: [e-mail]
1-Point Support Europe
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
1-Point Support Japan
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Rest of the World
Please contact your local distributor.