GE Amersham ECl Gel Box User manual

GE Healthcare
Life Sciences
Amersham™ ECL™ Gel Box
User Manual

Table of Contents
51 Introduction ........................................................................................
61.1 Protein gel electrophoresis overview .................................................................
71.2 Important user information ...................................................................................
91.3 Regulatory information ............................................................................................
112 Safety ...................................................................................................
122.1 Safety precautions .....................................................................................................
182.2 Labels ...............................................................................................................................
212.3 Recycling information ...............................................................................................
222.4 Declaration of Hazardous Substances (DoHS) ..............................................
263 System description ............................................................................
273.1 Amersham ECL Gel Box ............................................................................................
303.2 Amersham ECL Gel .....................................................................................................
344 Installation ..........................................................................................
385 Protocols ..............................................................................................
395.1 Electrophoresis protocol ..........................................................................................
445.2 Removing the gel from the cassette ..................................................................
465.3 Post-staining of the gel ............................................................................................
535.4 Transfer protocol .........................................................................................................
576 Maintenance .......................................................................................
597 Troubleshooting .................................................................................
628 Related products ................................................................................
Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC 3
Table of Contents

1 Introduction
General information
Amersham ECL Gel Box together with Amersham ECL Gel constitute a
ready-to-use mini-gel system for high quality separation of proteins.
Amersham ECL Gel is compatible with Tris-Glycine buffer and contains no
SDS, making it ideal for protein analysis under both native and denaturing
conditions. Amersham ECL Gel is compatible with standard protein detection
protocols, and has an extended shelf-life for increased convenience.
Scope of this document
These instructions provide information on how to perform polyacrylamide
gel electrophoresis (PAGE) using Amersham ECL Gel Box and Amersham
ECL Gel. Additionally, protocols for post-staining and transfer are provided.
In this chapter
This chapter contains the following sections:
See
page
Section
61.1 Protein gel electrophoresis overview
71.2 Important user information
91.3 Regulatory information
Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC 5
1 Introduction

1.1 Protein gel electrophoresis overview
Below is a flowchart of protein analysis including separation of proteins
using PAGE followed by either Western blotting or post-staining detection
methods.
Western blotting
detection method
Post-staining
detection method
Transfer
Detection
Post-staining
Detection
Gel electrophoresis
6 Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
1 Introduction
1.1 Protein gel electrophoresis overview

1.2 Important user information
Intended use
Amersham ECL Gel Box and Amersham ECL Gel are intended for PAGE.
Amersham ECL Gel Box and Amersham ECL Gel are intended for research
use only, and shall not be used in any clinical procedures, or for diagnostic
purposes.
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES
concerning the safe use of Amersham ECL Gel Box. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if
not avoided, could result in death or serious injury. It
is important not to proceed until all stated conditions
are met and clearly understood.
Cautions
CAUTION
CAUTION indicates a hazardous situation which, if
not avoided, could result in minor or moderate injury.
It is important not to proceed until all stated conditions
are met and clearly understood.
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1 Introduction
1.2 Important user information

Notices
NOTICE
NOTICE indicates instructions that must be followed
to avoid damage to the product or other equipment.
8 Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
1 Introduction
1.2 Important user information

1.3 Regulatory information
Introduction
This section describes the directives and standards that are fulfilled by
Amersham ECL Gel Box.
CE Conformity
This product complies with the European directives listed in the table below,
by fulfilling the corresponding harmonized standards. A copy of the
Declaration of Conformity is available on request.
TitleDirective
Low Voltage Directive (LVD)2006/95/EC
Electromagnetic Compatibility (EMC) Directive2004/108/EC
CE Marking
The CE marking and the corresponding declaration of conformity, is valid
for the instrument when it is:
•used as a stand-alone unit, or
•connected to other CE marked GE Healthcare instruments, or
•connected to other products recommended or described in the user
documentation, and
•used in the same state as it was delivered from GE Healthcare, except
for alterations described in the user documentation.
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1 Introduction
1.3 Regulatory information

International
standards
This product fulfills the requirements of the following standards:
NotesDescriptionStandard
Harmonized
with
2006/95/EC
Safety requirements for
electrical equipment for
measurement, control, and
laboratory use.
EN 61010-1, IEC
61010-1, UL 61010-
1, CAN/CSA-C22.2
No.61010-1
Harmonized
with
2006/95/EC
Safety requirements for hand-
held probe assemblies for
electrical measurement and test.
EN 61010-031,
IEC 61010-031
Harmonized
with
2004/108/EC
EMC emissions and immunity
requirements for electrical
equipment for measurement,
control and laboratory use.
EN 61326-1
Regulatory compliance
of connected
equipment
Any equipment connected to Amersham ECL Gel Box should meet the safety
requirements of EN 61010-1/IEC 61010-1, or other relevant harmonized
standards. Within EU, connected equipment must be CE marked.
10 Amersham ECL Gel and Gel Box User Manual 28-9943-33 AC
1 Introduction
1.3 Regulatory information