Leica CM1860 User manual

Instructions for Use
CM1860/CM1860 UV - Cryostat
Leica CM1860/CM1860 UV, V1.6 RevI– 10/2016, English
Order no.: 14 0491 80101 RevI
Always keep this manual with the instrument.
Read carefully before working with the instrument.

3
Leica CM1860/CM1860UV – Cryostat
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone:[phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
contract provisions agreed between ourselves
and our customers.
Leica reserves the right to change technical spec-
ifications as well as manufacturing processes
without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – includ-
ing any electronic systems and media – requires
express prior permission in writing by Leica Bio-
systems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the nameplate on
the back of the instrument.
© Leica Biosystems Nussloch GmbH
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field. We
are under no obligation to update the present
manual periodically and on an ongoing basis ac-
cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi-
vidual case, we shall not be held liable for errone-
ous statements, drawings, technical illustrations
etc. contained in this manual. In particular, no
liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information regarding the contents or technical
details of the present Instructions for Use are not
to be considered warranted characteristics of
our products. These are determined only by the
NOTE

4Instructions for Use V 1.6 RevI – 10/2016
Table of Contents
1. Important Information ............................................................................................................................. 6
1.1 Symbols in the text and their meanings ..............................................................................................................6
1.2 Qualification of personnel........................................................................................................................... 8
1.3 Intended use of instrument......................................................................................................................... 8
1.4 Instrument type............................................................................................................................................. 8
2. Safety ......................................................................................................................................................... 9
2.1 Safety notes .................................................................................................................................................. 9
2.2 Warnings ....................................................................................................................................................... 9
2.3 Safety devices ............................................................................................................................................ 10
2.3.1 Locking/unlocking the handwheel .......................................................................................................... 12
2.3.2 Knife guard .................................................................................................................................................. 12
2.4 Operating conditions ................................................................................................................................. 13
2.5 Operating the instrument .......................................................................................................................... 14
2.6 Cleaning and disinfection ......................................................................................................................... 14
2.7 Removing the microtome .......................................................................................................................... 15
2.8 Maintenance............................................................................................................................................... 15
3. Technical Data ....................................................................................................................................... 16
4. Setting up the Instrument ..................................................................................................................... 18
4.1 Installation site requirements .................................................................................................................. 18
4.2 Unpacking instructions for the cryostat CM1860/1860UV................................................................... 19
4.3 Standard delivery—packing list .............................................................................................................. 22
4.4 Assembling the handwheel ...................................................................................................................... 23
5. Instrument Setup.................................................................................................................................... 24
5.1 Electrical connection................................................................................................................................. 24
5.2 Prior to operation ....................................................................................................................................... 24
5.3 Overview of the Leica CM1860/CM1860 UV ........................................................................................... 25
5.4 Power switch and circuit breaker........................................................................................................... 27
5.5 Switching on the instrument .................................................................................................................... 27
6. Operating the Instrument...................................................................................................................... 28
6.1 Control panel 1............................................................................................................................................ 28
6.2 Programming the desired values............................................................................................................. 31
6.2.1 Setting the time........................................................................................................................................... 31
6.2.2 Setting the automatic defrost time (cryochamber) .............................................................................. 31
6.2.3 Selecting the cryochamber temperature............................................................................................... 31
6.2.4 Activation of the Peltier element (option) .............................................................................................. 32
6.2.5 Manual defrosting of the quick freeze shelf.......................................................................................... 32
6.2.6 Manual defrosting of the cryochamber.................................................................................................. 33
6.2.7 Setting the section thickness................................................................................................................... 33
6.3 Retraction (factory default setting: disabled).......................................................................... 33
6.4 Display lock ................................................................................................................................................. 34
6.5 Control panel 2 - Electric coarse feed .................................................................................................... 34

5
Leica CM1860 /CM1860UV – Cryostat
7. Daily Use of the Instrument.................................................................................................................. 35
7.1 Specimen freezing ..................................................................................................................................... 35
7.1.1 Quick freeze shelf....................................................................................................................................... 35
7.1.2 Stationary heat extractor (optional) ............................................................................................ 36
7.2 Specimen discs .......................................................................................................................................... 36
7.2.1 Inserting the specimen discs into the specimen head ........................................................................ 36
7.2.2 Specimen orientation ................................................................................................................................ 36
7.3 Sectioning.................................................................................................................................................... 37
7.3.1 Blade holder CE .......................................................................................................................................... 37
7.3.2 Blade holder CE-TC.................................................................................................................................... 43
7.3.3 Knife holder CN........................................................................................................................................... 44
7.3.4 Knife holder CN-Z with anti-roll guide .................................................................................................... 48
7.3.5 Cleaning the blade and knife holders ..................................................................................................... 48
7.3.6 Installing the shelf (movable) (optional) ................................................................................................. 50
7.4 Temperature selection chart (in minus°C) ............................................................................................ 51
7.5 Defrosting .................................................................................................................................................... 52
7.5.1 Automatic defrosting of the cryochamber............................................................................................. 52
7.5.2 Manual defrosting of the cryochamber.................................................................................................. 52
7.5.3 Manual defrosting of the quick freeze shelf.......................................................................................... 53
7.6 Terminating work ....................................................................................................................................... 53
7.6.1 Terminating daily work.............................................................................................................................. 53
7.6.2 Shutdown for a longer period .................................................................................................................. 54
8. Troubleshooting ..................................................................................................................................... 55
8.1 Error messages in the display.................................................................................................................. 55
8.2 Temperature control button ..................................................................................................................... 56
8.3 Possible sources of error, causes and remedies ................................................................................. 57
8.3.1 Changing the battery ................................................................................................................................. 61
9. Cleaning, Disinfection, Maintenance ................................................................................................ 62
9.1 Cleaning ....................................................................................................................................................... 62
9.2 Spray disinfection with Leica Cryofect................................................................................................... 63
9.3 Maintenance............................................................................................................................................... 64
9.3.1 General maintenance instructions.......................................................................................................... 64
9.3.2 Replacing the fuses ................................................................................................................................... 66
9.3.3 Replacing the UVC lamp............................................................................................................................ 66
9.3.4 Replacing the fluorescent lamp............................................................................................................... 68
10. Ordering Information, Components and Consumables................................................................... 69
10.1 Ordering information.................................................................................................................................. 69
10.2 Mobile heat extractor – application............................................................................................ 92
10.3 Cold extractor – application ........................................................................................................ 92
11. Warranty and Service ........................................................................................................................... 93
12. Decontamination Certificate (Master)............................................................................................... 94
Table of Contents

6Instructions for Use V 1.6 RevI – 10/2016
1.1 Symbols in the text and their meanings
Useful tips,
i.e. important information for the user,
appear in gray boxes and are marked
by an .
Caution – UVC radiation!
Numbers in parentheses refer to item
numbers in illustrations or to the illus-
trations themselves.
(5)
(Fig.5)
1. Important Information
Warnings
appear in a gray box and are marked
by a warning triangle .
REF
SN
Manufacturer
This product fulfills the requirements
of the Council's Directive 98/79/EC
concerning in vitro diagnostics (IVD)
medical devices.
In vitro diagnostics (IVD) medical device
Observe the Instructions for Use
Order No.
Serial number
The CSA test mark means that a prod-
uct has been tested and fulfills the
applicable safety and/or performance
standards, including the relevant stan-
dards defined or administered by the
American National Standards Institute
(ANSI), Underwriters Laboratories (UL),
the Canadian Standards Association
(CSA), the National Sanitation Founda-
tion International (NSF) and others.
Symbol for labeling electrical and elec-
tronic equipment in accordance with
Section 7 of the German Electrical and
Electronic Equipment Act (ElektroG).
ElektroG is the law governing how
electrical and electronic equipment
is brought into circulation, returned
and disposed of in an environmentally
friendly manner.
Environmental protection symbol of the
China RoHS directive. The number in
the symbol indicates the "Environment-
friendly Use Period" of the product. The
symbol is used if a substance restricted
in China is used in excess of the maxi-
mum permitted limit.
Date of Manufacture

7
Leica CM1860/CM1860UV – Cryostat
The package contents are fragile and
must be handled with care.
Indicates the correct upright position of
the package.
The package must be kept in a dry en-
vironment.
Indicates the temperature
range permitted for storing
and handling the package.
Minimum +5 °C
Maximum +50 °C
Indicates the temperature
range permitted for transport-
ing the package.
Minimum -40 °C
Maximum +55 °C
It is not permitted to stack packages,
and no loads may be placed on top of
the package.
1. Important Information
Tip-n-Tell indicator to moni-
tor whether the shipment has
been transported and stored
in upright position according
to your requirements. With
a pitch of 60 ° or more, the
blue quartz sand flows into
the arrow-shaped indicator
window and sticks there per-
manently. Improper handling
of the shipment is immediately
detectable and can be proven
definitively.
Example of labeling
in accordance with
IPPC
Package labeling in accor-
dance with German Haz-
ardous Freight Ordinance
Road and Rail (GGVSE)/Eu-
ropean Agreement concern-
ing the International Car-
riage of Dangerous Goods
by Road (ADR) for trans-
porting hazardous goods.
Class 3: "FLAMMABLE LIQ-
UID"
• IPPCsymbol
• Countrycodein accordancewith
ISO 3166, e.g. DE for Germany
• Regionalidentifier,e.g.HEforHessen
• Producer/treatmentprovider code,
unique assigned number starting
with 49
• Treatmentcode,e.g.HT(heattreat-
ment), MB (methyl bromide), and
possibly DB (debarked)

8Instructions for Use V 1.6 RevI – 10/2016
1. Important Information
1.4 Instrument type
All information contained in these Instructions for
Use applies exclusively to the instrument type in-
dicated on the title page. A nameplate bearing the
instrument serial number (SN) is attached to the
back of the instrument.
These Instructions for Use include important
instructions and information related to the oper-
ating safety and maintenance of the instrument.
The Instructions for Use are an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near the
instrument.
If additional requirements on accident prevention
and environmental protection apply in the country
of operation, these Instructions for Use must be
supplemented by appropriate instructions to ensure
Fig. 1
1.3 Intended use of instrument
The Leica CM1860/CM1860UV is a high-performance cryostat with disinfection system for routine and
research applications in biology, medicine and industry. The instrument is used to freeze and section
specimen material quickly. The instrument is not designed for unsupervised storage of specimen
material. The instrument may only be operated within the scope of its designated use as described
above and as per the instructions given in these Instructions for Use. The Leica CM1860/CM1860UV
is also suitable for IVD (in vitro diagnostics).
Any other use of the instrument is considered improper.
Despite chemical and/or UV-light disinfection, adequate safety measures as per the applicable
laboratory regulations must still be taken (i.e. safety goggles, gloves, laboratory coat and mask
must be worn). These disinfections mean a reduction in the number of germs of at least 99.99 %
(see certificate on the Leica website).
1.2 Qualification of personnel
Only trained laboratory personnel may operate the Leica CM1860/CM1860 UV. Prior to operating the
instrument, the operator must thoroughly read and understand these Instructions for Use and must
familiarize him/herself with all technical details of the instrument.
The nameplate shown here is intended as an
example only!
compliance with such requirements.
Proper and intended use includes the observance
of all instructions in the Instructions for Use and
compliance with all inspection and maintenance
instructions.

9
Leica CM1860/CM1860UV – Cryostat
2. Safety
2.1 Safety notes
These Instructions for Use include important in-
structions and information related to the operating
safety and maintenance of the instrument.
The Instructions for Use are an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near the
instrument.
This instrument has been built and tested in accor-
dance with the safety requirements for electrical
equipment for measurement, control, and labora-
tory use.
Residual risks
The instrument has been designed and constructed
with the latest state-of-the-art technology and
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other
Leica products.
The protective devices on both instrument and components must neither be removed nor modi-
fied. Only service personnel qualified by Leica may repair the instrument and access the instru-
ment's internal components.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Operating the instrument safely is, above all, the responsibility of the owner,
as well as the designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
These Instructions for Use must be appropriately supplemented as required by the existing
regulations on accident prevention and environmental safety in the operator's country.
For the CE certificate and up-to-
date certificates pertaining to UV
disinfection, visit us online at:
www.LeicaBiosystems.com
according to recognized standards and regula-
tions with regard to safety technology. Operating
or handling the instrument incorrectly can place
the user or other personnel at risk of injury or can
cause damage to the instrument or other property.
The machine may be used only as intended and
only if all of its safety features are in proper working
condition. Malfunctions that impede safety must be
remedied immediately.
To maintain this condition and ensure safe opera-
tion, the user must observe all notes and warnings
contained in these Instructions for Use.
Only original spare parts and permitted original
accessories may be used.

10 Instructions for Use V 1.6 RevI – 10/2016
2. Safety
UVC disinfection is effective when disinfecting sur-
faces and air within the irradiated working area of the
Leica CM1850UV, CM1860UV and CM1950 cryostats at -20 °C
(Table 1, see I. Maier certificate). For powerful disinfection, we
recommend irradiation for three hours (CM1850UV/CM1860UV/
CM1950). Vegetative bacteria including Mycobacterium tuber-
culosis, bacterial endospores (Bacillus sp.) and fungi are killed
during this time. Even viruses including such resistant species
as hepatitis are inactivated up to at least 4 log10 units (99.99 %).
2.3 Safety devices
To prevent adverse health effects from UV radiation, the UV dis-
infection cycle can be started only after the sliding window has
been properly closed. Closing the window activates the corre-
sponding safety features.
When working with cryostats, personal safety precautions must
always be taken. It is mandatory to wear work safety shoes, safe-
ty gloves, a mask and safety goggles.
The consistent use of these safety features and strict observation of the
warnings and cautions in these Instructions for Use will safeguard the
operator from accidents and/or personal injury to a great extent.
Personal safety precautions
The instrument is equipped with the following safety devices: Handwheel
lock and knife guard on the knife holder.