Leica CM3050 S User manual

Leica CM3050 S
Cryostat
Instruction Manual
Leica CM3050 S  Cryostat
English V1.1  02/2000
Always kee this manual near the instrument.
Read carefully rior to o erating the instrument.

3
Leica CM 3050 S  Cryostat
Issued by:
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: htt ://www.hbu.de
Serial No.: .............. ......................................................
Year of Manufacture: ...............................................
Country of Origin: Federal Re ublic of Germany
Important note
The information contained in this document rep-
resents state-of-the-art technology as well as
the current state of knowledge.
Leica will not assume liability for errors that
might be contained in this manual, nor for acci-
dental damage or damage arising from the de-
livery, performance or use of this manual.
Therefore, no claims can be made based on the
text and illustrations in this instruction manual.
Leica Microsystems Nussloch mbH reserves
the right to change technical specifications
without notice since each of our products is
subject to a policy of continuous improvement.
This document is protected under copyright
laws. Leica Microsystems Nussloch mbH
retains all rights related to this documentation.
Any reproduction of text and illustrations - or
any parts thereof - in form of printing, photo-
copying, microfiches, or other methods, includ-
ing electronic systems, requires the prior writ-
ten permission of Leica Microsystems Nussloch
mbH.
The serial number and the year of manufacture
are specified on the nameplate at the back of
the instrument.
©Leica Microsystems Nussloch mbH

4Instruction Manual V1.1 02/2000
Table of Contents
1. Important information.................................................................................................................. 7
1.1 Symbols used in this manual and ........................................................................................................... 7
1.2 Designated use.......................................................................................................................................... 8
1.3 Qualification of personnel........................................................................................................................ 8
2.1 eneral information on instrument ........................................................................................................ 9
2. Safety .............................................................................................................................................. 9
2.2 Integrated safety devices ........................................................................................................................ 9
2.2.1 Locking the handwheel ......................................................................................................................... 10
2.2.2 Centering the handwheel grip .............................................................................................................. 10
2.2.3 Emergency stop function ...................................................................................................................... 11
2.2.4 Knife guard .............................................................................................................................................. 11
2.3 Safety instructions: handling and operation of the instrument........................................................ 12
2.3.1 Transport ................................................................................................................................................. 12
2.3.2 Site requirements ................................................................................................................................... 12
2.3.3 Electrical connections........................................................................................................................... 12
2.3.4 Handling microtome knives/blades ..................................................................................................... 13
2.3.5 Knife guard/handwheel lock................................................................................................................. 13
2.3.6 Motorized sectioning ............................................................................................................................. 13
2.3.7 Defrosting/ ............................................................................................................................................... 13
2.3.8 Frozen parts of the instrument and...................................................................................................... 13
2.3.9 Infectious/radioactive material ............................................................................................................ 14
2.3.10 Disinfection and cleaning ................................................................................................................... 14
2.3.11 Removing/reinstalling the microtome ............................................................................................... 15
2.3.12 Display message Dry microtome ..................................................................................................... 15
2.3.13 Maintenance ......................................................................................................................................... 15
3. Installation................................................................................................................................... 16
3.1 Site requirements.................................................................................................................................... 16
3.1.1 eneral site requirements .................................................................................................................... 16
3.1.2 Electrical connections ........................................................................................................................... 16
3.2 Unpacking and installation .................................................................................................................... 16
3.3 Standard delivery .................................................................................................................................... 17

5
Leica CM3050 S  Cryostat
Table of Contents
3.4 Installing/inserting accessories ........................................................................................................... 18
3.4.1 Installing the handwheel ....................................................................................................................... 18
3.4.2 Inserting the accessories ..................................................................................................................... 18
3.4.3 The footswitch ........................................................................................................................................ 19
3.5 Prior to switching on the instrument .................................................................................................... 19
4. Instrument Properties ................................................................................................................ 20
4.1 Overview................................................................................................................................................... 20
4.2 Technical Data......................................................................................................................................... 22
5. Operation ..................................................................................................................................... 24
5.1 Operating the instrument for the first time .......................................................................................... 24
5.2 Switching on/fuses ................................................................................................................................. 25
5.3 Control panel 1......................................................................................................................................... 26
5.3.1 Key functions in control panel 1........................................................................................................... 26
5.3.2 Display functions in control panel 1 .................................................................................................... 26
5.3.3 Display indications when switching on .............................................................................................. 27
5.3.4 Menu functions: ...................................................................................................................................... 28
5.3.5 Status display.......................................................................................................................................... 31
5.3.6 Indication of refrigeration state, section thickness setting and counters .................................... 32
5.4 Control panel 2......................................................................................................................................... 33
5.4.1 Selecting a sectioning mode / .............................................................................................................. 34
5.4.2 Setting the sectioning window............................................................................................................. 35
5.4.3 Selecting sectioning speed .................................................................................................................. 36
5.4.4 Emergency stop ...................................................................................................................................... 36
5.4.5 Coarse feed keys .................................................................................................................................... 37
5.4.6 Trimming and sectioning functions ..................................................................................................... 38
5.4.7 Setting the section thickness ............................................................................................................... 38
5.5 Daily operation of the instrument ......................................................................................................... 39
5.5.1 Freezing specimens onto specimen .................................................................................................... 39
5.5.2 Freezing specimens onto specimen .................................................................................................... 40
5.5.3 Optional accessories for freezing........................................................................................................ 40
5.5.4 Inserting specimen discs into the........................................................................................................ 41

6Instruction Manual V1.1 02/2000
Table of contents
5.5.5 Specimen orientation............................................................................................................................. 41
5.5.6 Trimming / sectioning ............................................................................................................................ 42
5.6 Finishing work.......................................................................................................................................... 43
5.6.1 Daily routine steps.................................................................................................................................. 43
5.6.2 Switching off the instrument for a ....................................................................................................... 43
5.7 Defrosting ................................................................................................................................................. 44
5.7.1 Automatic chamber defrost cycle ....................................................................................................... 44
5.7.2 Manual chamber defrost cycle ............................................................................................................ 45
5.7.3 Defrosting the specimen head ............................................................................................................. 45
6. Troubles ooting, applications tips ......................................................................................... 46
6.1 Displayed error messages ..................................................................................................................... 46
6.1.1 Other error indications .......................................................................................................................... 46
6.2 Potential problems - causes and remedies ........................................................................................ 47
6.3 Reference chart for temperature settings (in minus °C).................................................................. 51
7. Disinfection, cleaning and maintenance .............................................................................. 52
7.1 Safety instructions .................................................................................................................................. 52
7.2 Spray disinfection with Leica Cryofect................................................................................................ 52
7.3 Disinfection with conventional ............................................................................................................. 53
7.4 Cleaning .................................................................................................................................................... 53
7.5 Removing / reinstalling the microtome ................................................................................................ 54
7.5.1 Removing the microtome ...................................................................................................................... 54
7.5.2 Disassembling the specimen head...................................................................................................... 54
7.5.3 Reinstalling the microtome ................................................................................................................... 55
7.6 Exchanging the fluorescent light lamp ................................................................................................ 56
8. Warranty and service ................................................................................................................ 57
8.1 Warranty................................................................................................................................................... 57
8.2 Technical service information .............................................................................................................. 57
8.2.1 Important information for repairs / returns of cryostats .................................................................. 58
8.3 Shutdown and disposal of the instrument........................................................................................... 58
Appendix............................................................................................................................................... 59

7
Leica CM 3050 S  Cryostat
T e c apters of t e Leica CM3050 S cryostat
instruction manual:
Chapter 1 Structure of the manual:
 Table of contents
 Important information on this
manual
Chapter 2 Safety
 Read this chapter before
operating the instrument!
Chapter 3 Installation
 Unpacking and installation
 Standard delivery, information
on accessories
Chapter 4 Instrument properties
 Overview
 Technical data
Chapter 5 Operation
 Controls
 Setup, daily use and shut
down
Chapter 6 Trouble shooting
an applications tips
 Operating errors
 Display error messages
 Temperature reference chart
Chapter 7 Disinfection, cleaning
an maintenance
Chapter 8 Warranty an service
Appen ix Electrohy raulic height a justment
Or ering information accessories
EC Declaration of Conformity
Alphabetical in ex
1. Important information
1.1 Symbols used in t is manual and
Warnings
appear in a grey box an are
marke by a warning triangle .
Notes
i.e. important user information ap-
pears in a grey box an is marke
by an information symbol.
Figures in brackets refer to item
numbers in rawings or to the
rawings themselves.
Instrument type:
All information given in this instruction manual
applies only to the instrument type indicated on
the title page.
A name plate, indicating the instrument serial
number, is attached to the back of the instru-
ment.
Require information for all inquiries:
For any inquiries please specify:
 Instrument type
 Serial number
(5)
(Fig.5)
t eir meaning

8Instruction Manual V1.1 02/2000
General
This instruction manual includes important in-
structions and information related to the operat-
ing safety and maintenance of the instrument.
The instruction manual is an important part of
the product. It must be read carefully before us-
ing the instrument for the first time and must al-
ways be kept with the instrument.
If additional requirements, which exceed the
scope of this manual, are imposed by regula-
tions and/or laws on accident prevention and
environmental protection in the country of oper-
ation, appropriate instructions for compliance
with such requirements must be added to this
manual.
Read this instruction manual carefully before
attempting to use or operate the instrument.
Please pay particular attention to
chapter 2 (safety features, safety in-
structions). - Please rea this infor-
mation, even if you are alrea y fa-
miliar with the operation an use of
other Leica pro ucts.
1. Important information
1.2 Designated use
The Leica CM3050 S is a powerful cryostat for
routine as well as research applications in biol-
ogy, medicine and industry.
The instrument has been designed for rapid
freezing and sectioning of tissue samples.
The instrument has not been designed for un-
attended storage of tissue material.
The instrument may only be operated within the
scope of its designated use as described above
and as per the instructions given in this manual.
Any other use of the instrument is considered
improper.
1.3 Qualification of personnel
The Leica CM3050 S may only be operated by
trained laboratory personnel.
All laboratory personnel designated to operate
the instrument must carefully read the present
instruction manual prior to starting work with
the instrument.

9
Leica CM3050 S  Cryostat
2. Safety
2.1 General information on instrument
This instrument has been built and tested in ac-
cordance with the following safety regulations
on electrical measuring, control, regulating and
laboratory devices:
 DIN EN 292
 DIN EN 61010-1
 EN 50082-1
 EN 55011
 IEC 1000-4
as well as according to the international quality
standard
 DIN ISO 9001
In order to maintain this condition and to ensure
safe operation, the operator must observe the
instructions and warnings contained in this in-
struction manual.
2.2 Integrated safety devices
The instrument is equipped with the following
safety devices:
 Handwheel lock
 Handwheel grip centering
 Emergency stop function
(instruments with sectioning motor only)
 Knife holder equipped with knife guard
The safety devices installed by the manufactu-
rer of the instrument only constitute the basis of
accident prevention.
Mainly responsible for accident-free operation
is above all the institution which owns the in-
strument and, in addition, the designated per-
sonnel who operate, service or repair the
instrument.
design and safe andling

10 Instruction Manual V1.1 02/2000
Instruments with sectioning motor:
The sectioning motor is now blocked.
All instruments:
The message LOCKED in the display of control
panel 1 indicates that the handwheel has been
locked:
Unlocking:
 To unlock, push locking pin (2) to the left into
position (2a).
 Display indication LOCKED disappears.
Instruments with sectioning motor:
The sectioning motor can now be activated
again.
2.2.2 Centering t e andw eel grip
During motorize sectioning, for
safety reasons always center the
han wheel grip!
 To center grip (1), pull outwards and pivot
into center of handwheel.
 When released, the grip locks into position.
2.2.1 Locking t e andw eel
Always cover the cutting e ge with
the knife guar an lock the han -
wheel:
 Prior to oing any work on
knife an /or specimen.
 Prior to exchanging specimens.
 During work breaks.
The handwheel can be locked in 2 positions:
 with the grip in the uppermost position (left),
 with the grip in the lowest position (right).
Locking:
 Rotate handwheel, until grip (1) is in upper or
lower position.
 To lock, press pin (2) to the right into position
(2b).
--> The upper locking position for pin (2) is
marked by a black dot (4).
2. Safety
2
2
CT- 3 0 °C OT - 35°C
LOCKED