Leica Cm3050 s User manual

Leica CM3050 S

Cryostat

Instruction Manual

Leica CM3050 S  Cryostat

English V1.1  02/2000

Always kee this manual near the instrument.

Read carefully rior to o erating the instrument.

Leica CM 3050 S  Cryostat

Issued by:

Leica Microsystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: +49 6224 143-0

Fax: +49 6224 143-200

Internet: htt ://www.hbu.de

Serial No.: .............. ......................................................

Year of Manufacture: ...............................................

Country of Origin: Federal Re ublic of Germany

Important note

The information contained in this document rep-

resents state-of-the-art technology as well as

the current state of knowledge.

Leica will not assume liability for errors that

might be contained in this manual, nor for acci-

dental damage or damage arising from the de-

livery, performance or use of this manual.

Therefore, no claims can be made based on the

text and illustrations in this instruction manual.

Leica Microsystems Nussloch mbH reserves

the right to change technical specifications

without notice since each of our products is

subject to a policy of continuous improvement.

This document is protected under copyright

laws. Leica Microsystems Nussloch mbH

retains all rights related to this documentation.

Any reproduction of text and illustrations - or

any parts thereof - in form of printing, photo-

copying, microfiches, or other methods, includ-

ing electronic systems, requires the prior writ-

ten permission of Leica Microsystems Nussloch

mbH.

The serial number and the year of manufacture

are specified on the nameplate at the back of

the instrument.

©Leica Microsystems Nussloch mbH

4Instruction Manual V1.1 02/2000

Table of Contents

1. Important information.................................................................................................................. 7

1.1 Symbols used in this manual and ........................................................................................................... 7

1.2 Designated use.......................................................................................................................................... 8

1.3 Qualification of personnel........................................................................................................................ 8

2.1 eneral information on instrument ........................................................................................................ 9

2. Safety .............................................................................................................................................. 9

2.2 Integrated safety devices ........................................................................................................................ 9

2.2.1 Locking the handwheel ......................................................................................................................... 10

2.2.2 Centering the handwheel grip .............................................................................................................. 10

2.2.3 Emergency stop function ...................................................................................................................... 11

2.2.4 Knife guard .............................................................................................................................................. 11

2.3 Safety instructions: handling and operation of the instrument........................................................ 12

2.3.1 Transport ................................................................................................................................................. 12

2.3.2 Site requirements ................................................................................................................................... 12

2.3.3 Electrical connections........................................................................................................................... 12

2.3.4 Handling microtome knives/blades ..................................................................................................... 13

2.3.5 Knife guard/handwheel lock................................................................................................................. 13

2.3.6 Motorized sectioning ............................................................................................................................. 13

2.3.7 Defrosting/ ............................................................................................................................................... 13

2.3.8 Frozen parts of the instrument and...................................................................................................... 13

2.3.9 Infectious/radioactive material ............................................................................................................ 14

2.3.10 Disinfection and cleaning ................................................................................................................... 14

2.3.11 Removing/reinstalling the microtome ............................................................................................... 15

2.3.12 Display message Dry microtome ..................................................................................................... 15

2.3.13 Maintenance ......................................................................................................................................... 15

3. Installation................................................................................................................................... 16

3.1 Site requirements.................................................................................................................................... 16

3.1.1 eneral site requirements .................................................................................................................... 16

3.1.2 Electrical connections ........................................................................................................................... 16

3.2 Unpacking and installation .................................................................................................................... 16

3.3 Standard delivery .................................................................................................................................... 17

Leica CM3050 S  Cryostat

Table of Contents

3.4 Installing/inserting accessories ........................................................................................................... 18

3.4.1 Installing the handwheel ....................................................................................................................... 18

3.4.2 Inserting the accessories ..................................................................................................................... 18

3.4.3 The footswitch ........................................................................................................................................ 19

3.5 Prior to switching on the instrument .................................................................................................... 19

4. Instrument Properties ................................................................................................................ 20

4.1 Overview................................................................................................................................................... 20

4.2 Technical Data......................................................................................................................................... 22

5. Operation ..................................................................................................................................... 24

5.1 Operating the instrument for the first time .......................................................................................... 24

5.2 Switching on/fuses ................................................................................................................................. 25

5.3 Control panel 1......................................................................................................................................... 26

5.3.1 Key functions in control panel 1........................................................................................................... 26

5.3.2 Display functions in control panel 1 .................................................................................................... 26

5.3.3 Display indications when switching on .............................................................................................. 27

5.3.4 Menu functions: ...................................................................................................................................... 28

5.3.5 Status display.......................................................................................................................................... 31

5.3.6 Indication of refrigeration state, section thickness setting and counters .................................... 32

5.4 Control panel 2......................................................................................................................................... 33

5.4.1 Selecting a sectioning mode / .............................................................................................................. 34

5.4.2 Setting the sectioning window............................................................................................................. 35

5.4.3 Selecting sectioning speed .................................................................................................................. 36

5.4.4 Emergency stop ...................................................................................................................................... 36

5.4.5 Coarse feed keys .................................................................................................................................... 37

5.4.6 Trimming and sectioning functions ..................................................................................................... 38

5.4.7 Setting the section thickness ............................................................................................................... 38

5.5 Daily operation of the instrument ......................................................................................................... 39

5.5.1 Freezing specimens onto specimen .................................................................................................... 39

5.5.2 Freezing specimens onto specimen .................................................................................................... 40

5.5.3 Optional accessories for freezing........................................................................................................ 40

5.5.4 Inserting specimen discs into the........................................................................................................ 41