Leica CM 1850 User manual

Instruction Manual
Leica CM 1850 V2.0 English – 03/2001
Always keep this man al near the instr ment!
Read caref lly prior to operating the instr ment!
Leica CM 1850
Cryostat

3
Leica CM 1850 – Cryostat
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 19
D 69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
eMail: histo_info@leica microsystems.com
Internet: http://www.histo solutions.com
Serial No.: ....................................................................
Year of Manufacture: ................................................
Country of Origin: .. Federal Republic of Germany
For erroneo s statements, drawings, technical il-
l strations etc. contained in this man al we ex-
cl de liability as far as permissible according to
the national legal system applicable in each indi-
vid al case. In partic lar, no liability whatsoever
is accepted for any financial loss or conseq en-
tial damage ca sed by or related to compliance
with statements or other information in this
man al.
Statements, drawings, ill strations and other in-
formation as regards contents or technical de-
tails of the present man al are not to be consid-
ered as warranted characteristics of o r
prod cts. These are determined only by the con-
tract provisions agreed between o rselves and
o r c stomers.
Leica reserves the right to change technical
specifications as well as man fact ring pro-
cesses witho t prior notice. Only in this way is it
possible to contin o sly improve the technology
and man fact ring techniq es sed in o r prod-
cts.
This doc ment is protected nder copyright laws.
Any copyrights of this doc ment are retained by
Leica Microsystems N ssloch GmbH.
Any reprod ction of text and ill strations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – incl d-
ing any electronic systems and media – req ires
express prior permission in writing by Leica
Microsystems N ssloch GmbH.
For the instr ment serial n mber and year of
man fact re, please refer to the name plate at
the back of the instr ment.
© Leica Microsystems N ssloch GmbH
The information, n merical data, notes and val e
j dgments contained in this man al represent the
c rrent state of scientific knowledge and state-
of-the-art technology as we nderstand it follow-
ing thoro gh investigation in this field. We are
nder no obligation to pdate the present man al
periodically and on an ongoing basis according
to the latest technical developments, nor to pro-
vide o r c stomers with additional copies, p-
dates etc. of this man al.
INFORMATION

4Instruction Manual V2.0 – 03/2001
Table of contents
1. Important Information .................................................................................................... 7
1.1 Symbols used in this manual and their meaning ......................................................... 7
1.2 Designated use .................................................................................................................. 8
1.3 Qualification of personnel ............................................................................................... 8
2. Safety................................................................................................................................. 9
2.1 Safety features ................................................................................................................. 9
2.1.1 Loc ing the handwheel................................................................................................... 9
2.1.2 Knife guard ........................................................................................................................ 9
2.2 General information on instrument design and safe handling ................................ 10
2.3 Operating conditions ..................................................................................................... 11
2.5 Cleaning and disinfection ............................................................................................. 12
2.6 Removal of the microtome ........................................................................................... 12
2.7 Maintenance................................................................................................................... 13
3. Technical data............................................................................................................... 14
4. Unpacking and installation ........................................................................................ 1
4.1 Site requirements........................................................................................................... 16
4.2 Transport to the desired location ................................................................................ 16
4.3 Standard delivery ........................................................................................................... 17
4.4 Handwheel assembly .................................................................................................... 18
5. Setup....................................................................................................................................
5.1 Connection to mains power ......................................................................................... 19
5.2 Prior to operation ...........................................................................................................19
5.3 Leica CM 1850 - Overview ............................................................................................ 20
5.4 Mains switch and automatic mains fuse ................................................................... 22
5.5 Turning the instrument on ............................................................................................ 22
. Control panel operation ............................................................................................... 23
6.1 Control panel 1................................................................................................................ 23
6.2 Programming the desired values ................................................................................ 23
6.2.1 Setting the time .............................................................................................................. 23
6.2.2 Setting the automatic defrost time (cryochamber) ................................................. 24
6.2.3 Selecting the cryochamber temperature .................................................................. 24
6.2.4 Activation of the Peltier element (option) .................................................................. 25
6.2.5 Manual defrosting of the quic freeze shelf ............................................................. 25

5
Leica CM1850 – Cryostat
Table of contents
6.2.6 Manual defrosting of the cryochamber ..................................................................... 26
6.3 Display loc ..................................................................................................................... 26
6.4 Control panel 2 - Electric coarse feed ........................................................................ 27
7. Daily use of the instrument ......................................................................................... 28
7.1 Specimen freezing ......................................................................................................... 28
7.1.1 Quic freeze shelf .......................................................................................................... 28
7.1.2 Stationary heat extractor (accessory)* ..................................................................... 28
7.2 Specimen discs .............................................................................................................. 29
7.2.1 Inserting the specimen discs in the specimen head ............................................... 29
7.2.2 Specimen orientation .................................................................................................... 29
7.3 Sectioning ....................................................................................................................... 30
7.3.1 Trimming the specimen................................................................................................. 30
7.3.2 Section thic ness setting ............................................................................................. 30
7.4 Temperaturew selection chart (in minus °C) ............................................................ 31
7.5 Defrosting ........................................................................................................................ 32
7.5.1 Automatic defrosting of the cryochamber ................................................................ 32
7.5.2 Manual defrosting of the cryochamber ..................................................................... 32
7.5.3 Manual defrosting of the quic freeze shelf ............................................................. 33
7.6 Terminating wor ........................................................................................................... 33
7.6.1 Terminating daily wor .................................................................................................. 33
7.6.2 Shutdown for a longer period ...................................................................................... 34
8. Troubleshooting ............................................................................................................ 34
8.1 Error messages in the display...................................................................................... 34
8.2 Temperature control button ......................................................................................... 35
8.3 Possible causes and remedies .................................................................................... 36
9. Cleaning, disinfection, maintenance........................................................................ 41
9.1 Cleaning ........................................................................................................................... 41
9.2 Spray disinfection with Leica Cryofect ...................................................................... 42
9.3 Maintenance................................................................................................................... 43
9.3.1 General maintenance .................................................................................................... 43
9.3.2 Removal of the microtome ........................................................................................... 44
9.3.3 Removal of the microtome cover ................................................................................ 46
9.3.4 Reinstallation of the microtome .................................................................................. 46

6Instruction Manual V2.0 – 03/2001
Table of contents
9.3.5 Replacement of the fuses ............................................................................................. 48
9.3.6 Replacement of the lamp .............................................................................................. 48
10. Ordering information, optional accessories ........................................................... 50
10.1 Ordering information ..................................................................................................... 50
10.2 Optional accessories..................................................................................................... 51
10.2.1 Mobile heat extractor.................................................................................................... 51
10.2.2 Thermal bloc ................................................................................................................. 51
11. Appendix ........................................................................................................................ 52

7
Leica CM 1850 – Cryostat
1.1 Symbols used in this manual and
thei meaning
Warnings
appear in a grey box and are marked
by a warning triangle .
Notes
i.e. important user information ap
pears in a grey box and is marked by
an information symbol.
Figures in brackets refer to item num
bers in drawings or to the drawings
themselves.
Instrument type:
All information given in this instr ction man al
applies only to the instr ment type indicated on
the title page.
A name plate, indicating the instr ment serial
n mber, is attached to the back of the instr ment.
Required information for all inquiries:
For any inq iries please specify:
• Instr ment type
• Serial n mber
The chapte s of the Leica CM1850 c yostat
inst uction manual:
Chapter 1 Structure of the manual:
• Table of contents
• Important information on this
man al
Chapter 2 Safety
• Read this chapter before
operating the instr ment!
Chapter 3 Technical data
• Unpacking and installation
• Standard delivery, informa-
tion on accessories
Chapter 4 Unpac ing and installation
• Site req irements
• Standard delivery
Chapter 5 Setup
• Overview
• T rning the instr ment on
Chapter 6 Control panel operation
• Control panel 1
• Programming the desired val-
es
• Control panel 2
Chapter 7 Daily use of the instrument
Chapter 8 Troubleshooting
Chapter 9 Cleaning, disinfection, mainte-
nance
Chapter 10 Ordering information, optional
accessories
Appendix EC Declaration
1. Important Information
(5)
(Fig.5)

8Instruction Manual V2.0 – 03/2001
Gene al
This instr ction man al incl des important instr ctions and information re-
lated to the operating safety and maintenance of the instr ment.
The instr ction man al is an important part of the prod ct. It m st be read
caref lly before sing the instr ment for the first time and m st always be
kept with the instr ment.
If additional req irements, which exceed the scope of this man al, are im-
posed by reg lations and/or laws on accident prevention and environmen-
tal protection in the co ntry of operation, appropriate instr ctions for com-
pliance with s ch req irements m st be added to this man al.
Read this instr ction man al caref lly before attempting to se or operate
the instr ment.
Please pay particular attention to chapter 2 (safety features,
safety instructions). Please read this information, even if you are
already familiar with the operation and use of other Leica prod
ucts.
1.2 Designated use
The Leica CM1850 is a powerf l cryostat for ro tine as well as research
applications in biology, medicine and ind stry.
The instr ment has been designed for rapid freezing and sectioning of tis-
s e samples.
The instr ment has not been designed for nattended storage of tiss e ma-
terial.
The instr ment may only be operated within the scope of its designated se
as described above and as per the instr ctions given in this man al.
Any other se of the instr ment is considered improper.
1.3 Qualification of pe sonnel
The Leica CM1850 may only be operated by trained laboratory personnel.
All laboratory personnel designated to operate the instr ment m st care-
f lly read the present instr ction man al prior to starting work with the in-
str ment.
1. Important Information

9
Leica CM1850 – Cryostat
2.1 Safety featu es
The instr ment and its accessory eq ipment incorporate the following safety
feat res: safety handwheel and knife g ards on the knife holders.
The consistent se of these safety feat res and strict observation of the
warnings and ca tions in this man al, will safeg ard the operator from ac-
cidents and/or personal inj ry to a great extent.
2.1.1 Locking the handwheel
Prior to manipulating the knife and specimen, or changing the
specimen or knife, and during breaks, always lock the handwheel!
For locking the handwheel rotate the handle ntil it is in the pper position.
P sh the locking pin (1) into the recess at the handwheel. The locking posi-
tion is marked by a black dot (2). If necessary, move the handwheel slightly
forth and back ntil the locking mechanism engages.
To nlock, p sh the locking pin (1) to the left from the recess at the handwheel.
2.1.2 Knife gua d
For every manipulation in the cryochamber, or upon changing a
specimen when the knife or disposable blade is clamped, or dur
ing breaks, cover the cutting edge with the knife guard.
The knife holders CN and CS are eq ipped with knife g ards; on the knife
holder CE the glass plate of the anti-roll g ide constit tes the knife g ard
(please refer to the separate instr ction man al for yo r knife holder). Prior
to manip lating the knife and specimen, or changing the specimen or knife,
and d ring breaks, always lock the handwheel!
2. Safety
Fig. 10.1
Fig. 10.2
2
1

10 Instruction Manual V2.0 – 03/2001
2.2 Gene al info mation on inst ument design and safe handling
This instr ment has been b ilt and tested in accordance with the following
safety reg lations on electrical meas ring, control, reg lating and labora-
tory devices:
• DIN EN 292,
• DIN EN 61010-1,
• EN 50082-1,
• EN 55011,
• IEC 1000-4
as well as according to the international q ality standard,
• DIN ISO 9001.
In order to maintain this condition and to ens re safe operation, the opera-
tor m st observe the instr ctions and warnings contained in this instr ction
man al.
2.3 Ope ating conditions
T anspo t and installation
• After transporting do not t rn the instr ment on for a minim m of 4 ho rs!
• Do not operate in rooms with explosion hazard!
• To ens re an adeq ate cooling capacity, the instr ment m st be set p
with at least 10 cm distance from walls and f rnit re!
Connection to mains
• Before connecting to the mains power, please check if the local voltage
complies with the power rating specified on the name plate of the instr -
ment!
• D ring the compressor start- p the nominal voltage m st not drop be-
low the val es specified in the ‘Technical data‘!
The compressor req ires a start- p c rrent between 45 and 50 A.
Therefore, the electric circ it at the place of installation m st be inspected
by an electrical engineer to ens re that it meets the req irements for a
smooth operation of the instr ment.
2. Safety

11
Leica CM1850 – Cryostat
2. Safety
A constant adeq ate power s pply to the instr ment m st be ens red at
all times.
Fail re to comply with the above will ca se severe damage to the instr -
ment.
• After transporting, wait at least 4 ho rs before t rning the instr ment on.
This waiting period is necessary to allow the compressor oil, which may
have been displaced d ring transport, to ret rn to its original position.
Fail re to comply with this can ca se severe damage to the instr ment.
Def osting
• The q ick freeze shelf may become hot d ring defrosting!
Therefore, do not to ch it!
2.4 Ope ating the inst ument
• Take care when handling microtome knives and disposable blades. The
c tting edge is extremely sharp and can ca se severe inj ry!
• Never leave knives and knife holders with a knife/blade mo nted lying
aro nd!
• Do not place a knife on a table with the c tting edge facing pward!
• Never try to catch a falling knife!
• Always clamp the specimen before the knife!
• Prior to manip lating the knife and specimen, or changing the specimen
or knife, and d ring breaks, always lock the handwheel and cover the
c tting edge with the knife g ard!
• Avoid contact with cold parts of the instr ment as this can ca se frost-
bite!
• To make s re that the condensation water stemming from the defrost
cycles drains into the waste container and to avoid the risk of possible
contaminations, ens re that the tap of the waste container (2, Fig. 31.1) is
open when operating the instr ment. Only sh t the tap when draining
the waste container!

12 Instruction Manual V2.0 – 03/2001
2.5 Cleaning and disinfection
• It is not necessary to remove the microtome for ro tinely disinfecting the
cryochamber.
The instr ment is appropriate for spray disinfection with Leica Cryofect!
• Do not se organic solvents or any other aggressive s bstances for clean-
ing and disinfection!
Only se the cleaning agents and disinfectants specified in this instr c-
tion man al s ch as Leica Cryofect (alcohol or common disinfectants
based on alcohol)!
2.6 Removal of the mic otome
• Prior to removing the microtome, t rn the instr ment off with the mains
switch and p ll the mains pl g.
• Prior to removing the microtome, bring the specimen head to the lower
position with the handwheel.
Otherwise the specimen head wo ld rapidly fall down and might inj re
the operator‘s hands, when taking o t the microtome.
• Wear appropriate protective gloves to take the cold microtome o t of
the cryochamber.
Extended skin contact with cold parts of the instr ment may ca se frost
bite!
• The microtome m st be entirely dry before reinstallation. H midity inside
will condense and freeze in the cold cryostat and th s ca se malf nc-
tions or damage.
• Do not se external heaters for drying the cryochamber. This can ca se
damage to the cooling system!
• All components removed from the cryostat m st be caref lly dried be-
fore they are replaced in the cryochamber.
2. Safety

13
Leica CM1850 – Cryostat
2.7 Maintenance
Replacement of the fuses
• T rn the instr ment off with the a tomatic mains f se and p ll the mains
pl g, before replacing the f ses.
• Only se f ses of the same specification! For the req ired val es, please
refer to Chapter 3 ‘Technical data‘.
The se of f ses other than specified by the man fact rer may ca se
severe damage to the instr ment!
Replacement of the lamp
• T rn the instr ment off with the a tomatic mains f se and p ll the mains
pl g, before replacing the lamp.
• If the lamp is broken, it m st be replaced by the technical service, as the
replacement involves a high risk of inj ry.
2. Safety

14 Instruction Manual V2.0 – 03/2001
Type CM1850-11 CM1850-03 CM1850-07 CM 1850-08 CM 1850-01
Mark of conformity CUL VDE
Nominal voltage (±10%) 100 V AC 120 V AC 230 V AC 240 V AC 230 V AC
Nominal frequency 50 Hz/60 Hz 60 Hz 60 Hz 50 Hz 50 Hz
Power draw 1600 VA 1600 VA 1600 VA 1600 VA 1600 VA
Max. start up current for 5 sec 45A eff. 45A eff. 45A eff. 45A eff. 45A eff.
Protective class I IIII
Automatic mains fuse T15A M3 T12A T1 T10A T1 T10A T1 T10A T1
Pollution degree 2 2222
Overvoltage installation category II II II II II
Heat emission (max.) 1600 J/s 1600 J/s 1600 J/s 1600 J/s 1600 J/s
Refrigeration system 50 Hz 60 Hz
Cryochamber
Temperature setting range 0 °C to 35 °C (+ 2 K / 0 K) 0 °C to 35 °C (+ 2 K / 0 K)
Defrosting automatic hot gas defrosting, automatic hot gas defrosting,
temperature controlled temperature controlled
1 automatic defrost cycle/24 hours, 1 automatic defrost cycle/24 hours,
temperature controlled temperature controlled
manual defrost cycle manual defrost cycle
Refrigeration capacity 690 W 690 W
Safety factor 3 3
Refrigerant 300 g ±5g refrigerant R 404A *300 g ±5g refrigerant R 404A *
Compressor oil 0.6 l EMKARATE RL 22S, ICI *0.6 l EMKARATE RL 22S, ICI *
Quick freeze shelf
Max. temperature 40 °C (+ 0 K / 2 K) 40°C (+ 0 K / 2 K)
Number of quick freeze stations 10 10
Defrosting manual hot gas defrosting manual hot gas defrosting
time controlled time controlled
Peltier element (option)
Max. temperature 60 °C (+5 K) 60 °C (+5 K)
Number of freezing stations 2 2*
Defrosting together with quick freeze shelf together with quick freeze shelf
Operating temperature range (ambient temperature): 18 °C to 40 °C.
All specifications related to temperature are valid only up to an ambient temperature of 22 °C
and an air humidity lower than 60%!
3. Technical data

15
Leica CM 1850 – Cryostat
Please refer to section 5.2 ‘Site require
ments‘!
3. Technical data
All CM 1850 types require the following fuses:
F1: T0,25 A Fa. Sch rter, Typ FST; 6,3x32 mm oder T0,25 A Fa. Wickmann, Typ 19343; 6,3x32 mm
F2: T0,6 A Fa. Sch rter, Typ FST; 6,3x32 mm oder T0,6 A Fa. Wickmann, Typ 19343; 6,3x32 mm
F3: T1,6 A Fa. Sch rter, Typ FST; 6,3x32 mm oder T1,6 A Fa. Wickmann, Typ 19343; 6,3x32 mm
F4: T6,25 A Fa. Sch rter, Typ FST; 6,3x32 mm oder T6,25 A Fa. Wickmann, Typ 19343; 6,3x32 mm
F5: T4 A Fa. Sch rter, Typ FST; 6,3x32 mm oder T4 Fa. Wickmann, Typ 19343; 6,3x32 mm
* Refrigerant and compressor oil must only be replaced by qualified, authorized service per
sonnel!
Microtome
Rotary microtome
Section thickness setting 1 - 60 µm
Specimen feed 25 mm
Vertical stroke 59 mm
Maxim m specimen size 55 x 55 mm
Specimen orientation 8° (x-, y-, z-Achse)
Electric coarse feed
slow 0.2 mm/s
rapid 0.7 mm/s
Cryocabinet
Dimensions
Width (w/o handwheel) 600 mm
Width (with handwheel) 730 mm
Depth 730 mm
Height 1140 mm
Weight (incl. microtome,
witho t specimen cooling) approx.135 kg
Fluorescent lamp
50 Hz version: Osram D l x S 11 W/21
Color: LUMILUX light white
60 Hz version: Osram D l x S 13 W/21
Color: LUMILUX light white
➀ according to IEC-1010, UL 3101
➁ according to CECOMAF
Liq id temperat re 45°C
Evaporation temperat re -25 °C

16 Instruction Manual V2.0 – 03/2001
4.1 Site equi ements
Do not operate in rooms with explosion hazard!To ensure an ad
equate cooling capacity, the instrument must be set up with at
least 10 cm distance from walls and furniture.
• The place of installation m st meet the following req irements:
– no direct s nlight,
– mains power socket at a distance no greater than approximately
1.5 m,
– no drafts (air condition o tlets, etc.) directly over the instr ment,
– even floor,
– mainly vibration-free floor,
– obstr ction-free access to the handwheel,
– room temperat re max. 35°C,
– Air h midity m st not exceed 60% High room temperat res and ex-
cessive air h midity affect the cooling capacity of the cryostat.
High room temperatures and excessive air humidity affect the
cooling capacity of the cryostat.
4.2 T anspo t to the desi ed location
4. Unpacking and installation
The instrument must be transported in
an upright position only.
When the instrument is tilted, the
compressor oil is displaced.
Do not grip the cabinet at the lid. Grip
the cabinet only at the marked loca
tions ( ).
Lengthwise
movement
Sideways
movement
Tilt
Fig. 16
15
14

17
Leica CM 1850 – Cryostat
• Move the instr ment to the installation site on its wheels (14). Please
note the areas which are reinforced for tarnsporting and grip the cabi-
net only at these locations (Fig. 16).
• The adj stable feet (15) can s pport the weight of the instr ment when
tipping at a slight angle (max. 30 °).
• At the installation site nscrew the screws in the adj stable feet (15)
sing a fork wrench.
This is necessary to ens re stability.
• Align the adj stable feet to level the instr ment.
The alignment of the adjustable feet is necessary to ensure an un
obstructed drain of the quick freeze shelf defrosting water.
4.3 Standa d delive y
The CM 1850 basic instr ment incl des the following:
- 1 orienting specimen head,
- 3 specimen discs, 25 mm ø,
- 1 bottle cryocompo nd, 125 ml,
- 1 bottle cryostat oil No. 407, 100 ml,
- 1 section waste tray,
- 1 storage shelf, left,
- 1 storage shelf right,
- 1 br sh shelf,
- 2 br shes,
- 1 handwheel (incl ding screw, spring washer,
self-adhesive cover disc),
- 1 tool set,
- 1 instr ction man al.
Instr ments with Peltier element also incl de the following:
- 1 Peltier element,
- 1 stationary heat extractor with s pport,
- 1 parking station.
4. Unpacking and installation

18 Instruction Manual V2.0 – 03/2001
Yo will find any f rther accessories (e.g. knife holder) yo may have or-
dered in the cardboard box as well.
Compare the delivered components with the parts list and yo r order. Sho ld
yo find any discrepancies, please contact yo r Leica sales office witho t
delay.
A choice of different knife holders is available for the CM 1850. The
knife holder is accompanied by its own separate instruction
manual.
Please contact your Leica sales office if the instruction manual is
missing.
4.4 Handwheel assembly
• Insert the pin (1) of the handwheel shaft in to
the hole (2) of the handwheel.
• Mo nt the spring washer (3) on the screw (4)
as shown in Fig. 18.
• Tighten the screw (4) with an Allen key (5 mm).
• Attach the cover disc (5) (self-adhesive).
To dismo nt, proceed in reverse order.
The handwheel including the fixing components are packed in the
cardboard box for the accessories.The handwheel can be dis
mounted for transporting (e.g. narrow doors).
Fig.18
4. Unpacking and installation
345
2
1

19
Leica CM1850 – Cryostat
5.1 Connection to mains powe
During the start up of the compressor the nominal voltage must not
drop below the values specified in the ‘Technical data‘.
Please note that the compressor requires a start up current be
tween 45 and 50 A.
Therefore, the electric circuit at the installation site must be in
spected by an electrical engineer to ensure that it meets the re
quirements for a smooth operation of the instrument.
A constant adequate power supply to the instrument must be en
sured at all times.
Failure to comply with the above will cause severe damage to the
instrument.
• The electric circ it at the place of installation m st be protected sepa-
rately.
• Do not connect any other cons mers to this electric circ it.
5.2 P io to ope ation
• Before connecting the instr ment to the mains power, please check if
the local mains voltage complies with the power rating indicated on the
name plate of the instr ment.
• Place the storage shelves in the cryochamber.
• Place the section waste tray and br sh shelf in the cryochamber.
• Place the knife holder base on the microtome base plate.
• Insert the knife holder and clamp it on the base plate For details, please
refer to the separate instr ction man al for yo r knife holder).
• Open the knife box with the knife and place it in the cryochamber for
precooling.
• Place all tools needed for specimen preparation in the cryochamber.
• Close the sliding window.
• Connect the mains pl g to the mains power o tlet at the wall.
5. Setup

20 Instruction Manual V2.0 – 03/2001
4
16
15
18
19
20
21
813
10
12
911
13
14
5.3 Leica CM 1850 - Ove view
5. Setup
13
17