Leica CM 1850 User manual

Instruction Manual
Leica CM 1850 V2.0 English – 03/2001
Always keep this man al near the instr ment!
Read caref lly prior to operating the instr ment!
Leica CM 1850
Cryostat

3
Leica CM 1850 – Cryostat
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 19
D 69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
eMail: histo_info@leica microsystems.com
Internet: http://www.histo solutions.com
Serial No.: ....................................................................
Year of Manufacture: ................................................
Country of Origin: .. Federal Republic of Germany
For erroneo s statements, drawings, technical il-
l strations etc. contained in this man al we ex-
cl de liability as far as permissible according to
the national legal system applicable in each indi-
vid al case. In partic lar, no liability whatsoever
is accepted for any financial loss or conseq en-
tial damage ca sed by or related to compliance
with statements or other information in this
man al.
Statements, drawings, ill strations and other in-
formation as regards contents or technical de-
tails of the present man al are not to be consid-
ered as warranted characteristics of o r
prod cts. These are determined only by the con-
tract provisions agreed between o rselves and
o r c stomers.
Leica reserves the right to change technical
specifications as well as man fact ring pro-
cesses witho t prior notice. Only in this way is it
possible to contin o sly improve the technology
and man fact ring techniq es sed in o r prod-
cts.
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Leica Microsystems N ssloch GmbH.
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any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – incl d-
ing any electronic systems and media – req ires
express prior permission in writing by Leica
Microsystems N ssloch GmbH.
For the instr ment serial n mber and year of
man fact re, please refer to the name plate at
the back of the instr ment.
© Leica Microsystems N ssloch GmbH
The information, n merical data, notes and val e
j dgments contained in this man al represent the
c rrent state of scientific knowledge and state-
of-the-art technology as we nderstand it follow-
ing thoro gh investigation in this field. We are
nder no obligation to pdate the present man al
periodically and on an ongoing basis according
to the latest technical developments, nor to pro-
vide o r c stomers with additional copies, p-
dates etc. of this man al.
INFORMATION

4Instruction Manual V2.0 – 03/2001
Table of contents
1. Important Information .................................................................................................... 7
1.1 Symbols used in this manual and their meaning ......................................................... 7
1.2 Designated use .................................................................................................................. 8
1.3 Qualification of personnel ............................................................................................... 8
2. Safety................................................................................................................................. 9
2.1 Safety features ................................................................................................................. 9
2.1.1 Loc ing the handwheel................................................................................................... 9
2.1.2 Knife guard ........................................................................................................................ 9
2.2 General information on instrument design and safe handling ................................ 10
2.3 Operating conditions ..................................................................................................... 11
2.5 Cleaning and disinfection ............................................................................................. 12
2.6 Removal of the microtome ........................................................................................... 12
2.7 Maintenance................................................................................................................... 13
3. Technical data............................................................................................................... 14
4. Unpacking and installation ........................................................................................ 1
4.1 Site requirements........................................................................................................... 16
4.2 Transport to the desired location ................................................................................ 16
4.3 Standard delivery ........................................................................................................... 17
4.4 Handwheel assembly .................................................................................................... 18
5. Setup....................................................................................................................................
5.1 Connection to mains power ......................................................................................... 19
5.2 Prior to operation ...........................................................................................................19
5.3 Leica CM 1850 - Overview ............................................................................................ 20
5.4 Mains switch and automatic mains fuse ................................................................... 22
5.5 Turning the instrument on ............................................................................................ 22
. Control panel operation ............................................................................................... 23
6.1 Control panel 1................................................................................................................ 23
6.2 Programming the desired values ................................................................................ 23
6.2.1 Setting the time .............................................................................................................. 23
6.2.2 Setting the automatic defrost time (cryochamber) ................................................. 24
6.2.3 Selecting the cryochamber temperature .................................................................. 24
6.2.4 Activation of the Peltier element (option) .................................................................. 25
6.2.5 Manual defrosting of the quic freeze shelf ............................................................. 25

5
Leica CM1850 – Cryostat
Table of contents
6.2.6 Manual defrosting of the cryochamber ..................................................................... 26
6.3 Display loc ..................................................................................................................... 26
6.4 Control panel 2 - Electric coarse feed ........................................................................ 27
7. Daily use of the instrument ......................................................................................... 28
7.1 Specimen freezing ......................................................................................................... 28
7.1.1 Quic freeze shelf .......................................................................................................... 28
7.1.2 Stationary heat extractor (accessory)* ..................................................................... 28
7.2 Specimen discs .............................................................................................................. 29
7.2.1 Inserting the specimen discs in the specimen head ............................................... 29
7.2.2 Specimen orientation .................................................................................................... 29
7.3 Sectioning ....................................................................................................................... 30
7.3.1 Trimming the specimen................................................................................................. 30
7.3.2 Section thic ness setting ............................................................................................. 30
7.4 Temperaturew selection chart (in minus °C) ............................................................ 31
7.5 Defrosting ........................................................................................................................ 32
7.5.1 Automatic defrosting of the cryochamber ................................................................ 32
7.5.2 Manual defrosting of the cryochamber ..................................................................... 32
7.5.3 Manual defrosting of the quic freeze shelf ............................................................. 33
7.6 Terminating wor ........................................................................................................... 33
7.6.1 Terminating daily wor .................................................................................................. 33
7.6.2 Shutdown for a longer period ...................................................................................... 34
8. Troubleshooting ............................................................................................................ 34
8.1 Error messages in the display...................................................................................... 34
8.2 Temperature control button ......................................................................................... 35
8.3 Possible causes and remedies .................................................................................... 36
9. Cleaning, disinfection, maintenance........................................................................ 41
9.1 Cleaning ........................................................................................................................... 41
9.2 Spray disinfection with Leica Cryofect ...................................................................... 42
9.3 Maintenance................................................................................................................... 43
9.3.1 General maintenance .................................................................................................... 43
9.3.2 Removal of the microtome ........................................................................................... 44
9.3.3 Removal of the microtome cover ................................................................................ 46
9.3.4 Reinstallation of the microtome .................................................................................. 46

6Instruction Manual V2.0 – 03/2001
Table of contents
9.3.5 Replacement of the fuses ............................................................................................. 48
9.3.6 Replacement of the lamp .............................................................................................. 48
10. Ordering information, optional accessories ........................................................... 50
10.1 Ordering information ..................................................................................................... 50
10.2 Optional accessories..................................................................................................... 51
10.2.1 Mobile heat extractor.................................................................................................... 51
10.2.2 Thermal bloc ................................................................................................................. 51
11. Appendix ........................................................................................................................ 52

7
Leica CM 1850 – Cryostat
1.1 Symbols used in this manual and
thei meaning
Warnings
appear in a grey box and are marked
by a warning triangle .
Notes
i.e. important user information ap
pears in a grey box and is marked by
an information symbol.
Figures in brackets refer to item num
bers in drawings or to the drawings
themselves.
Instrument type:
All information given in this instr ction man al
applies only to the instr ment type indicated on
the title page.
A name plate, indicating the instr ment serial
n mber, is attached to the back of the instr ment.
Required information for all inquiries:
For any inq iries please specify:
• Instr ment type
• Serial n mber
The chapte s of the Leica CM1850 c yostat
inst uction manual:
Chapter 1 Structure of the manual:
• Table of contents
• Important information on this
man al
Chapter 2 Safety
• Read this chapter before
operating the instr ment!
Chapter 3 Technical data
• Unpacking and installation
• Standard delivery, informa-
tion on accessories
Chapter 4 Unpac ing and installation
• Site req irements
• Standard delivery
Chapter 5 Setup
• Overview
• T rning the instr ment on
Chapter 6 Control panel operation
• Control panel 1
• Programming the desired val-
es
• Control panel 2
Chapter 7 Daily use of the instrument
Chapter 8 Troubleshooting
Chapter 9 Cleaning, disinfection, mainte-
nance
Chapter 10 Ordering information, optional
accessories
Appendix EC Declaration
1. Important Information
(5)
(Fig.5)

8Instruction Manual V2.0 – 03/2001
Gene al
This instr ction man al incl des important instr ctions and information re-
lated to the operating safety and maintenance of the instr ment.
The instr ction man al is an important part of the prod ct. It m st be read
caref lly before sing the instr ment for the first time and m st always be
kept with the instr ment.
If additional req irements, which exceed the scope of this man al, are im-
posed by reg lations and/or laws on accident prevention and environmen-
tal protection in the co ntry of operation, appropriate instr ctions for com-
pliance with s ch req irements m st be added to this man al.
Read this instr ction man al caref lly before attempting to se or operate
the instr ment.
Please pay particular attention to chapter 2 (safety features,
safety instructions). Please read this information, even if you are
already familiar with the operation and use of other Leica prod
ucts.
1.2 Designated use
The Leica CM1850 is a powerf l cryostat for ro tine as well as research
applications in biology, medicine and ind stry.
The instr ment has been designed for rapid freezing and sectioning of tis-
s e samples.
The instr ment has not been designed for nattended storage of tiss e ma-
terial.
The instr ment may only be operated within the scope of its designated se
as described above and as per the instr ctions given in this man al.
Any other se of the instr ment is considered improper.
1.3 Qualification of pe sonnel
The Leica CM1850 may only be operated by trained laboratory personnel.
All laboratory personnel designated to operate the instr ment m st care-
f lly read the present instr ction man al prior to starting work with the in-
str ment.
1. Important Information

9
Leica CM1850 – Cryostat
2.1 Safety featu es
The instr ment and its accessory eq ipment incorporate the following safety
feat res: safety handwheel and knife g ards on the knife holders.
The consistent se of these safety feat res and strict observation of the
warnings and ca tions in this man al, will safeg ard the operator from ac-
cidents and/or personal inj ry to a great extent.
2.1.1 Locking the handwheel
Prior to manipulating the knife and specimen, or changing the
specimen or knife, and during breaks, always lock the handwheel!
For locking the handwheel rotate the handle ntil it is in the pper position.
P sh the locking pin (1) into the recess at the handwheel. The locking posi-
tion is marked by a black dot (2). If necessary, move the handwheel slightly
forth and back ntil the locking mechanism engages.
To nlock, p sh the locking pin (1) to the left from the recess at the handwheel.
2.1.2 Knife gua d
For every manipulation in the cryochamber, or upon changing a
specimen when the knife or disposable blade is clamped, or dur
ing breaks, cover the cutting edge with the knife guard.
The knife holders CN and CS are eq ipped with knife g ards; on the knife
holder CE the glass plate of the anti-roll g ide constit tes the knife g ard
(please refer to the separate instr ction man al for yo r knife holder). Prior
to manip lating the knife and specimen, or changing the specimen or knife,
and d ring breaks, always lock the handwheel!
2. Safety
Fig. 10.1
Fig. 10.2
2
1

10 Instruction Manual V2.0 – 03/2001
2.2 Gene al info mation on inst ument design and safe handling
This instr ment has been b ilt and tested in accordance with the following
safety reg lations on electrical meas ring, control, reg lating and labora-
tory devices:
• DIN EN 292,
• DIN EN 61010-1,
• EN 50082-1,
• EN 55011,
• IEC 1000-4
as well as according to the international q ality standard,
• DIN ISO 9001.
In order to maintain this condition and to ens re safe operation, the opera-
tor m st observe the instr ctions and warnings contained in this instr ction
man al.
2.3 Ope ating conditions
T anspo t and installation
• After transporting do not t rn the instr ment on for a minim m of 4 ho rs!
• Do not operate in rooms with explosion hazard!
• To ens re an adeq ate cooling capacity, the instr ment m st be set p
with at least 10 cm distance from walls and f rnit re!
Connection to mains
• Before connecting to the mains power, please check if the local voltage
complies with the power rating specified on the name plate of the instr -
ment!
• D ring the compressor start- p the nominal voltage m st not drop be-
low the val es specified in the ‘Technical data‘!
The compressor req ires a start- p c rrent between 45 and 50 A.
Therefore, the electric circ it at the place of installation m st be inspected
by an electrical engineer to ens re that it meets the req irements for a
smooth operation of the instr ment.
2. Safety