Leica CM1950 User manual

Instructions for Use
Leica CM1950 , 1v4 English – 10/2012
Order number 14 0477 80101 RevE
Always keep these instructions near the instrument!
Read carefully before working with the instrument.
Leica CM1950
Cryostat

3
Leica CM1950 – Cryostat
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
products. These are determined only by the con-
tract provisions agreed between ourselves and
our customers.
Leica reserves the right to change technical spec-
ifications as well as manufacturing processes
without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photo-
copy, microfiche, web cam or other methods
– including any electronic systems and media
– requires express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the type plate at the
rear side of the instrument.
© Leica Biosystems Nussloch GmbH
The information, numerical data, notes and value
judgments contained in this manual represent
the current state of scientific knowledge and
state-of-the-art technology as we understand it
following thorough investigation in this field. We
are under no obligation to update the present
manual periodically and on an ongoing basis ac-
cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi-
vidual case, we shall not be held liable for errone-
ous statements, drawings, technical illustrations
etc. contained in this manual. In particular, no
liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other in-
formation as regards contents or technical details
of the present Instructions for Use are not to be
considered as warranted characteristics of our
1. NOTE

4Instructions for Use 1v4 – 10/2012
Table of Contents
1. Important information... ................................................................................................6
1.1 Symbols in the text and their meanings.......................................................................6
1.2 Instrument type................................................................................................................6
1.3 Qualification of personnel..............................................................................................7
1.4 Intended use of the instrument... ..................................................................................7
2. Safety and design... .......................................................................................................8
2.1 Safety notes......................................................................................................................8
2.2 Warnings...........................................................................................................................8
2.3 General safety notes.......................................................................................................9
2.4 Unpacking and installation.............................................................................................9
2.5 Safety devices................................................................................................................12
2.6 Securing/locking the handwheel................................................................................13
2.7 Cleaning, disinfection – Turning the instrument back on. ......................................14
2.8 Handling specimens – Defrosting...............................................................................15
2.9 Removing the microtome..............................................................................................15
2.10 Maintenance... ...............................................................................................................15
3. Technical data..............................................................................................................16
4. Standard delivery.........................................................................................................18
5. General Overview........................................................................................................20
5.1 Control panel fields and cryostat chamber...............................................................21
6. Installation... .................................................................................................................22
6.1 Site requirements. .........................................................................................................22
6.2 Transport to the site......................................................................................................23
6.3 Assembling the handwheel..........................................................................................24
6.3.1 Locking/unlocking the handwheel..............................................................................25
6.3.2 Installing the footswitch dummy (instruments with cutting motor). .....................25
6.4 Electrical connection....................................................................................................26
6.5 Installing accessories/inserting chamber accessories..........................................27
6.5.1 Installing the adjustable footrest (optional). .............................................................27
6.5.2 Installing the storage systems (optional)...................................................................28
6.5.4 Inserting the section waste tray. ................................................................................28
6.5.3 Shelf, movable (optional)..............................................................................................28
6.5.5 Installing the heat extractor, stationary (optional). .................................................29
6.5.6 Installing the knife/blade holder and adjusting the clearance angle. ..................29
6.5.7 Inserting/changing the bacteria filter. .......................................................................31

5
Leica CM1950 – Cryostat
Table of Contents
6.5.8 Assembling the filter bag........................................................................................................... 31
6.5.9 Installing the section extraction (optional) – Use with blade holder CE only................... 32
7. Instrument controls... .............................................................................................................. 33
7.1 Control panel fields on the CM1950 – Control panel field 1. ............................................... 33
Control panel field 2 – Electric coarse feed, sectioning and
trimming thickness display........................................................................................................ 35
Control panel field 3 – Motorized sectioning (optional)... .................................................... 37
8. Working with the Instrument................................................................................................. 39
8.1 Preparing cutting tools, specimen discs and preparation aids.......................................... 39
8.2 Switching on the instrument..................................................................................................... 39
8.3 Configuring the parameters...................................................................................................... 40
8.4 Working with the precooled cryostat...................................................................................... 45
8.4.1 Preparatory work......................................................................................................................................45
8.4.2 Trimming with extraction – 1. Anti-roll guide installed......................................................... 46
8.4.3 Cutting with extraction – Anti-roll guide installed... ............................................................. 49
9. Troubleshooting... .................................................................................................................... 51
9.1 Problems during work................................................................................................................ 51
10. Temperature selection chart (in minus °C)... ...................................................................... 55
11. Optional accessories... ........................................................................................................... 56
11.1 Ordering information.................................................................................................................. 57
12. Maintenance and cleaning.................................................................................................... 71
12.1 General maintenance instructions. ......................................................................................... 71
12.2 Changing fuses............................................................................................................................ 73
12.3 Replacing the UVC lamp............................................................................................................ 73
12.3.1 Replacing the fluorescent lamp... ............................................................................................ 75
13. Decontamination Certificate (Master)................................................................................. 76
14. Warranty and service.............................................................................................................. 78

6Instructions for Use 1v4 – 10/2012
Factory No.
Cat. No.
This product fulfills the requirements
of the Council's Directive 98/79/EC
concerning in vitro diagnostics (IVD)
medical devices.
Serial number
Order No.
Symbol for labeling electrical and elec-
tronic equipment in accordance with
Section 7 of the German Electrical and
Electronic Equipment Act (ElektroG).
ElektroG is the law regarding the sale,
return and environmentally sound
disposal of electrical and electronic
equipment.
Environmental protection symbol of the
China RoHS directive. The number in
the symbol indicates the "Environment-
friendly Use Period" of the product. The
symbol is used if a substance restricted
in China is used in excess of the maxi-
mum permitted limit.
1. Important information
1.1 Symbols in the text and their meanings
Useful tips,
i.e. important user information, appear
in a gray box and are marked by an
.
Caution – UVC radiation!
Numbers in parentheses refer to item
numbers in illustrations.
(5)
(Fig. 5)
Warnings
appear in a gray box and are marked
by a warning triangle .
1.2 Instrument type
All information given in these Instructions for Use
applies only to the instrument type indicated on
the title page. A name plate indicating the instru-
ment serial number is attached to the back of the
instrument.
Fig. 1

7
Leica CM1950 – Cryostat
Despite chemical and/or UV-light dis-
infection, personal safety precautions
as per the applicable laboratory regu-
lations must still be taken (e.g. safety
goggles, gloves, laboratory coat and
mask must be worn).
This type of disinfection reduces the
number of germs by at least 99.99%.
1.3 Qualification of personnel
The Leica CM1950 may be operated by trained
laboratory personnel only.
Prior to operating the instrument, the operator
must thoroughly read and understand this instruc-
tion manual and must familiarize him/herself with
all technical details of the instrument.
1.4 Intended use of the instrument
The CM1950 is a high-performance cryostat with
an encapsulated microtome and separate speci-
men cooling. It features a UV disinfection system,
an (optional) integrated extraction system for
section waste and an (optional) motor for motor-
ized sectioning.
The cryostat is designed to produce frozen
sections for biological, medical and industrial
applications.
The CM1950 is suitable for in-vitro diagnostic
(IVD) applications.
The instrument may only be operated within the
scope of its designated use as described above
and as per the instructions given in these Instruc-
tions for Use.
Any other use of the instrument is
considered improper.
1. Important Information

8Instructions for Use 1v4 – 10/2012
2.1 Safety notes
These Instructions for Use include important in-
structions and information related to the operating
safety and maintenance of the instrument.
The Instructions for Use are an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near
the instrument.
This instrument has been built and test-
ed in accordance with the safety require-
ments for electrical equipment for mea-
surement, control, and laboratory use.
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other
Leica products.
The protective devices located on the instrument and the accessories must not be removed or
modified. The instrument must only be opened and repaired by service technicians authorized
by Leica.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Operating the instrument safely is, above all, the responsibility of the owner,
as well as the designated personnel who operate, service or repair the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
These Instructions for Use must be appropriately supplemented as required by the existing
regulations on accident prevention and environmental safety in the operator's country.
For the CE certificate and up-to-date
certificates pertaining to UV disinfec-
tion, visit us online at:
www.LeicaBiosystems.com
To maintain this condition and ensure safe opera-
tion, the user must observe all notes and warnings
contained in these Instructions for Use.
2. Safety and design

9
Leica CM1950 – Cryostat
2.3 General safety notes
The CM1950 is a cryostat with an encapsulated microtome and separate
specimen cooling. It is primarily used for work in the area of fast-cut diag-
nostics.
The displays and instrument controls are easy to operate due to their largely
self-explanatory symbols. LED displays make it easy to read. The cryocham-
ber is made of seamlessly welded, high-quality stainless steel that is free of
difficult-to-access corners and thus easy to clean and disinfect.
2.4 Unpacking and installation
• Toensureproperfunctionoftheinstrument,itmustbesetupwitha
minimum distance on all sides from walls and furniture (see "Site Require-
ments", p. 22).
• Theinstrumentmayonlybetransportedinanuprightorslightlyinclined
position.
• Toensure asafe transportwith afork lift3 peopleare required:one
operating the fork lift, and the other 2 holding the instrument on either
side to prevent it from sliding down.
• Beforeconnectingtothepowersupplysystem,pleaseobserve"Technical
data"!
• Neverconnecttheinstrumenttoapowersocketthatdoesnothavea
protective conductor terminal.
Length of the power cable: up to 3.5 m extension possible: No
After transporting, wait at least 4 hours before turning the instrument on. This waiting period is
necessary to allow the compressor oil, which may have been displaced during transport, to return
into its original position. Any condensation on electrical parts that has formed due to temperature
differences during transport must be allowed to dry completely. Failure to comply with this can
cause severe damage to the instrument!
2. Safety and design

10 Instructions for Use 1v4 – 10/2012
2. Safety and design
Opening the packaging
1. Remove the 3 screws (1) on each of the two sides of the
box (7) at the top.
2. Remove the 2 screws on each of the 4 hinges (2) on the
front and rear side of the box.
Do NOT remove the screws of the hinges on the
top lid!
3. Remove 1 screw (3) between the two hinges on the front
and rear side of the box.
4. Remove the 3 screws (4) at the bottom on all four sides
of the box.
5. Of the two vertical rows of screws (5), remove one on
either side (which side does not matter) entirely.
22
7
13
When the instrument is delivered, check the tilt indicators on the
packaging.
If the arrowhead is blue, the shipment was transported laying flat,
was tilted at too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment for
possible damage.
Fig. 2
4
5
5
4
8
8
8
8
8
17
9
9
Cover = ramp
10
14
11
11
Fig. 3

11
Leica CM1950 – Cryostat
2. Safety and design
Removing the packaging
1. Remove the cover (17) and place it on the floor with the foam parts facing
downwards – the cover is later used as a ramp.
2. Remove the 4 foam parts (6) by pulling them upwards.
3. Open the box (7) on its side hinges like an accordion partition and remove it.
4. Remove the accessories carton (8) and Instructions for Use on the front
of the instrument.
5. Pull off the dust cover (9) by pulling upwards.
6. Take off the foam strip (10) on the bottom rear side.
Ramp assembly
1. After the foam strip (10) is removed, 2 screws (12) are visible on the
baseplate.
2. Swing out the cover hinges (2) at the front and rear.
3. Attach the cover (17) from the rear to the transport pallet (11).
The notches (13) of the hinges (2) must be pointing towards the instrument.
4. Move the cover towards the left so that the notches (13) of the hinges
slide beneath the heads of the screws (12).
Transport to the Installation Location
The instrument must be transported in an upright position.
The cover (17) now forms a ramp on which the instrument can be rolled
down off the transport pallet.
Do not push the device by its hood (16)! Use the transport grip
points (15 )!
The rollers (14) must run over the cover hinges (2) at the front and
rear. Danger of tipping!
1. Carefully roll the instrument backwards over the ramp from the pallet.
2. Push the instrument to the installation location on the castors (14).
13
12
6
6 6
6
17
16
14
22
Fig. 5
Fig. 4
Fig. 6
15

12 Instructions for Use 1v4 – 10/2012
To prevent adverse health effects from UV radiation, the UV disinfec-
tion cycle can be started only after the sliding window has been prop-
erly closed. Closing the window activates the corresponding safety
features.
The consistent use of these safety features and strict observation of the warn-
ings and cautions in these Instructions for Use will safeguard the operator from
accidents and/or personal injury to a great extent.
Microtome knives
• Takecarewhen handlingmicrotomeknives/disposable blades.The
cutting edge is extremely sharp and can cause serious injuries!
• Neverleaveknivesandknifeholderswithaknife/blademountedlying
around!
• Neverplaceaknifeonatablewiththecuttingedgefacingupward!
We strongly recommend using the safety gloves included with the
standard delivery!
• Never try to catch a falling knife!
• Beforehandlingthespecimenortheknife,orchangingthespecimen,lock
the handwheel and ensure that the knife is covered by the knife guard.
• Avoidcontactwithcoldpartsoftheinstrumentasthiscancausefrost-
bite – wear the safety gloves supplied!
2. Safety and design
2.5 Safety devices
The Instructions for Use include important instructions and information related
to the operating safety and maintenance of the instrument.
These Instructions for Use are an important part of the product, and must be read
carefully before startup and use and must always be kept near the instrument.
If additional requirements on accident prevention and environmental protection
apply in the country of operation, these Instructions for Use must be supplement-
ed by appropriate instructions to ensure compliance with such requirements.
The instrument is equipped with the following safety devices: an emergency stop
switch (motorized instruments only), a handwheel lock and centering system
(motorized instruments only), knife guard on the blade and knife holder, and a
blade ejector.

13
Leica CM1950 – Cryostat
2.6 Securing/locking the handwheel
Always lock the handwheel prior to making modifications to the
knife or specimen, changing the specimen, or taking a break!
To lock the handwheel, press the lever (1) outward. Continue turning the
handwheel slowly until the grip is in the upper or lower position and the
handwheel is locked. Press the lever fully outward; gently rock the handwheel
back and forth until the locking mechanism clicks into place noticeably.
To release the handwheel, press the lever (2) on the handwheel toward the
cryostat housing.
Centering of the handwheel (motorized instruments only)
Pull out the handwheel's handle and position it in the middle of the hand-
wheel. The handle automatically engages in this position.
An important safety device on the cryostat is the centering of the
handwheel for motorized instruments.
Rotate the handwheel only if the refrigeration system is on and the
cryochamber is cold.
12 o'clock
position
6 o'clock
position
1
2
Fig. 8
Fig. 7
Fig. 9
2. Safety and design
Knife guard
Prior to making modifications to the knife and specimen, changing
the specimen or knife, or taking a break, always lock the hand-
wheel and cover the cutting edge with the knife guard!
The CE, CN and CN-Z knife holders feature knife guards; the glass anti-roll
plate of the CE knife holder also serves as a knife guard.

14 Instructions for Use 1v4 – 10/2012
2. Safety and design
• Whendisinfectingtheinstrument,takeappropriateprotectivemeasures
(gloves, mask, protective clothing, etc.).
• Whenusingdetergentsanddisinfectantspleasecomplywiththesafety
precautions of the disinfectant manufacturer!
• Theintegratedglassanti-rollguideofthebladeholdersCE,CNandCN-Z
can be cleaned either with acetone or alcohol.
• Disposeofwasteliquidaccordingtothewastedisposalregulations!
• Donotuseexternalheatersfordryingthecryochamber.Thiscancause
damage to the cooling system!
• Donotturntheinstrumentonbeforethecryochamberiscompletelydry!
Frost formation!
• Allcomponentsremovedfromthecryostatmustbecarefullydriedbefore
returning them to the cryochamber!
• Thefrontpanelandtheslitcoverofthemicrotomemustbecompletely
dry before turning on the instrument!
For more detailed information about disinfection, visit the
Leica Biosystems Division website at
www.LeicaBiosystems.com
It is not necessary to remove the microtome for disinfection.
• TheinstrumenthasbeendesignedforUVdisinfection!Spraydisinfection
with Leica Cryofect is also possible, thanks to the special insulation of
the microtome. (Cryofect is not available in all countries!)
Remove section waste after EVERY sectioning operation and
BEFORE changing specimens. Remove the section waste using a
paper towel soaked in Cryofect or an alcohol-based disinfectant)
or remove the waste using the extraction nozzle (optional). Do not
start the disinfection before swiveling the anti-roll guide to the
side! Each new specimen is a potential source of contamination.
2.7 Cleaning, disinfection – Turning the instrument back on

15
Leica CM1950 – Cryostat
2. Safety and design
2.9 Removing the microtome
• Themicrotomeisencapsulatedandthereforedoesnotrequireremoval
by the user.
2.10 Maintenance
Replacing the fuses
• Turntheinstrumentoffanddisconnectthepowerplugbeforereplacing
the fuses!
• UseonlythefusetypesspecifiedinChapter3,"Technicaldata".Theuse
of fuses other than specified by the manufacturer may cause severe
damage to the instrument!
Replacement of the lamp/UVC lamp
• Turntheinstrumentoffanddisconnectthepowerplugbeforereplacing
the lamps.
It is possible to break the UVC lamp during replacement. If this
happens, the lamp change must be completed by Technical
Service. If any metallic mercury is released, handle it carefully and
dispose of it properly.
If both disinfection indicator lights are blinking alternately, the UV
tube must be replaced!
2.8 Handling specimens – Defrosting
• Whenworkingwithcontaminatedorinfectedmaterial,thegeneralsafety
guidelines for laboratories must be applied!
• Beforedefrostingthecryochamberremoveallsamples!
• Beforedefrostingthespecimenhead,removeallsamples!
Never leave samples in the cryochamber! – The instrument is not
suitable for storing frozen specimens, as the refrigeration dehy-
drates the specimens!
The quick freeze shelf can become very hot during the defrosting
process. Therefore, do not touch it!

16 Instructions for Use 1v4 – 10/2012
3. Technical data
All specifications related to temperature are valid only up to an ambient temperature of 18°C
to 35 °C and a relative humidity of no more than 60%.
For "Site Requirements", refer to item 6.1
(page 20).
Instrument type -1 -2 -3
Nominal voltage (±10%) 100 VAC 120 VAC 230 VAC
Nominal frequency 50/60 Hz 60 Hz 50 Hz
Power draw 1900 VA 1900 VA 1900 VA
Max. start-up current for 5 sec. 35 A eff. 35 A eff. 25 A eff.
Protective class I I I
Automatic cutout T15A M3 T15A T1 T10A T1
Pollution degree2 2 2
Overvoltage category II II II
Heat emission (max.) 1900J/s 1900J/s 1900J/s
Approval CE CE/c_CSA_us CE
 in acc. with IEC-1010; UL 3101
Microtome Cryostat
Type Rotary microtome
encapsulated
Dimensions
700 mm
Width (excluding handwheel)
Section thickness range 1 to 100 µm Width (including handwheel) 835 mm
Trimming range Depth (cabinet only) 850 mm
Clinic 10 – 40 µm Height (total) 1215 mm
Research 1 – 600 µm Working height (armrest) 1025 mm
Total specimen feed 25 mm + 1 mm Weight
Vertical stroke 59 mm ±0.5 mm Weight (w/motor and extr.) 193 kg
Specimen retraction 20 µm (can be deactivated) Weight (w/motor, w/o extr.) 185 kg
Maximum specimen size 50 x 80 mm Weight (w/o motor, w/extr.) 183 kg
Cutting speed slow: 0 – 50 strokes/min
fast: 0 – 85 strokes/min
Vmax: 85 – 90 strokes/min
Weight (w/o motor, w/o extr.)
Weight (with specimen head cooling)
Weight (w/o specimen head cooling)
175 kg
165 kg
145 kg
Specimen orientation 8° (x-, y-axis)
Electric coarse feed slow: 300 µm/s
fast: 900 µm/s
General information
Operating temperature range 18°C to 35°C
Lamp 50/60Hz version: Osram
DULUX L 18W/840
Temperature range during storage
Relative humidity
+5°C to +55 °C
max. 60% non-
condensing
For additional details, see p. 32 Storage humidity < 60%

17
Leica CM1950 – Cryostat
3. Technical data
*) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only!
Refrigeration system CM1950, 50 Hz CM1950, 60 Hz
Cryochamber
Temperature range 0 °C to -35 °C ± 5 K, adjustable in 1K increments,
at an ambient temperature of 20 °C
0 °C to -35 °C ± 5 K, adjustable in 1K incre-
ments, ambient temperature of 20 °C
Cooling time to -25°C approx. 5 h approx. 5 h
Cooling time to -35 °C approx. 8 h approx. 8 h
Refrigeration capacity 690 W 690 W
Cut-out pressure 25 bar 25 bar
Safety factor 3 3
Refrigerant* 300 g (± 5 g), refrigerant R-404A* 300 g (± 5 g), refrigerant R-404A*
Compressor oil* 0.6 l EMKARATE RL22S, ICI* 0.6 l EMKARATE RL22S, ICI*
Defrosting of cryochamber
Automatic defrosting
Programmable Yes (hot gas defrost), selectable time Yes (hot gas defrost), selectable time
Defrosting intervals 1 defrost in 24 h
or manual hot gas defrost
1 defrost in 24 h
or manual hot gas defrost
Defrost time: 12 minutes 12 minutes
Automatic shutoff Defrost at a chamber temperature of -5 °C at chamber temperature -5 °C
Quick-freeze shelf
Minimum temperature -42 °C (+ 5 K), at chamber temp. -35°C -42 °C (+ 5 K), at chamber temp. -35°C
Number of freezing stations 15+2 15+2
Defrosting Manual hot gas defrost Manual hot gas defrost
Peltier element
Number of freezing stations 2 2
Max. temperature difference 17 K, at chamber temp. of -35 °C 17 K, at chamber temp. of -35 °C
Specimen cooling
Temperature range -10 to -50 °C ± 3 K -10 to -50 °C ± 3 K
Refrigeration capacity 320 W 320 W
Cut-out pressure 25 bar 25 bar
Safety factor 3 3
Refrigerant and quantity at 230 V/50Hz 130 g (± 5 g), refrigerant R-404A*
at 100 V/50/60 Hz 140 g (± 5 g), refrigerant R-404A*
at 120V/60Hz 140g (± 5g), refrigerant R-404A*
Compressor oil* 0.4 l alpha 22, Kyodo* 0.4 l alpha 22, Kyodo*
Defrosting of specimen head
Automatic defrosting No No
Manual defrost (defrost time) 15 min. 15 min.
in acc. with CECOMAF: Liquid temperature 45 °C, evaporation temperature: -25 °C

18 Instructions for Use 1v4 – 10/2012
4. Standard delivery
A choice of different blade/knife holders is available for the
CM1950.
Compare the delivered components with the parts list and your order. Should there be any
discrepancy, please contact the Leica Biosystems selling unit handling your order immediately.
Basic instrument WITHOUT motor/WITHOUT extraction, in the specific voltage variant
1 handwheel, manual .................................................................................. 14 0477 41346
5 specimen discs, 3 mm ............................................................................... 14 0477 40044
1 section waste tray ..................................................................................... 14 0477 40062
1 position holder for freeze shelf................................................................ 14 0477 40080
1 freeze shelf cover ...................................................................................... 14 0477 43763
1 tool set ......................................................................................................... 14 0436 43463
- 1 brush, fine........................................................................................... 14 0183 28642
- 1 Leica brush w/magnet ..................................................................... 14 0183 40426
- 1 Allen key, No. 1.5............................................................................... 14 0222 10050
- 1 Allen key, No. 2.5............................................................................... 14 0222 04137
- 1 Allen key, No. 3.0............................................................................... 14 0222 04138
- 1 Allen key, No. 4.0............................................................................... 14 0222 04139
- 1 Allen key with ball head, No. 4.0..................................................... 14 0222 32131
- 1 Allen key, No. 5.0............................................................................... 14 0222 04140
- 1 key with handle, No. 5.0................................................................... 14 0194 04760
- 1 Allen key, No. 6.0............................................................................... 14 0222 04141
- 1 Single-head wrench, No. 13 ........................................................... 14 0330 33149
- 1 Single-head wrench, No. 16 ........................................................... 14 0330 18595
1 power cable................................................................................................ 14 0441 xxxxx
1 bottle of cryostat oil, type 407, 50 ml....................................................... 14 0336 06098
1 bottle of OCT freezing compound, 125 ml .............................................. 14 0201 08926
1 pair of safety gloves, size M *, for cryosectioning............................... 14 0340 29011
1 Instructions for Use DE, EN + language CD ......................................... 14 0477 80001
1 DVD CM1950 "Troubleshooting".............................................................95.10696 Rev A
* Note: for the Japanese version: 100 V, 50/60 Hz; 1 pair of safety gloves, size S
(14 0340 40859) is included.
Basic instrument WITHOUT motor and WITH extraction,
standard scope of delivery as above,
plus:
1 accessory kit (extraction)......................................................................... 14 0477 43300
- Hose adapter 1 ..................................................................................... 14 0477 40293
- Hose adapter 2 ..................................................................................... 14 0477 40294
- Suction nozzle....................................................................................... 14 0477 40295
- Silicone hose ........................................................................................ 14 0477 43302
- Silicone stopper ................................................................................... 14 0477 43304
- Chamber suction nozzle...................................................................... 14 0477 43779
- Set of filters (5 pieces) ........................................................................ 14 0477 43792

19
Leica CM1950 – Cryostat
4. Standard Delivery
Basic instrument WITH motor/WITHOUT extraction, in the specific voltage variant
1 handwheel, motorized............................................................................... 14 0477 41347
5 specimen discs, 30 mm ............................................................................. 14 0477 40044
1 section waste tray ..................................................................................... 14 0477 40062
1 position holder for freeze shelf................................................................ 14 0477 40080
1 freeze shelf cover ...................................................................................... 14 0477 43763
1 tool set ......................................................................................................... 14 0436 43463
- 1 brush, fine........................................................................................... 14 0183 28642
- 1 Leica brush w/magnet ..................................................................... 14 0183 40426
- 1 Allen key, No. 1.5............................................................................... 14 0222 10050
- 1 Allen key, No. 2.5............................................................................... 14 0222 04137
- 1 Allen key, No. 3.0............................................................................... 14 0222 04138
- 1 Allen key, No. 4.0............................................................................... 14 0222 04139
- 1 Allen key with ball head, No. 4.0..................................................... 14 0222 32131
- 1 Allen key, No. 5.0............................................................................... 14 0222 04140
- 1 key with handle, No. 5.0................................................................... 14 0194 04760
- 1 Allen key, No. 6.0............................................................................... 14 0222 04141
- 1 Single-head wrench, No. 13 ........................................................... 14 0330 33149
- 1 Single-head wrench, No. 16 ........................................................... 14 0330 18595
1 power cable................................................................................................ 14 0441 xxxxx
1 bottle of cryostat oil, type 407, 50 ml ....................................................... 14 0336 06098
1 footswitch dummy ..................................................................................... 14 0443 30420
1 bottle of OCT freezing compound, 125 ml .............................................. 14 0201 08926
1 pair of safety gloves, size M *, for cryosectioning............................... 14 0340 29011
1 Instructions for Use DE, EN + language CD ......................................... 14 0477 80001
1 DVD CM1950 "Troubleshooting".............................................................95.10696 Rev A
* Note: for the Japanese version: 100 V, 50/60 Hz; 1 pair of safety gloves, size S
(14 0340 40859) is included.
Basic instrument WITH motor and WITH extraction, in the specific voltage variant
Standard scope of delivery as above,
plus:
1 accessory kit (extraction)......................................................................... 14 0477 43300
- Hose adapter 1 ..................................................................................... 14 0477 40293
- Hose adapter 2 ..................................................................................... 14 0477 40294
- Suction nozzle....................................................................................... 14 0477 40295
- Silicone hose ........................................................................................ 14 0477 43302
- Silicone stopper ................................................................................... 14 0477 43304
- Chamber suction nozzle...................................................................... 14 0477 43779
- Set of filters (5 pieces) ........................................................................ 14 0477 43792
A choice of different blade/knife holders is available for the
CM1950.
Compare the delivered components with the parts list and your order. Should there be any
discrepancy, please contact the Leica Biosystems selling unit handling your order immediately.

20 Instructions for Use 1v4 – 10/2012
5. General Overview
Castors for safe transport
over short distances
Handwheel in
12 o'clock
position
On/off switch, also
circuit breaker
Align adjusting feet for secure
upright position
Unscrew and align
adjusting feet after
transport
Bacteria filter
(optional, only for
instruments with filter)
Catch tank
for condensate
Footswitch
dummy
(motorized
instruments
only)
Emergency-stop
switch (motorized
instruments only)
Cryostat chamber
(with activated
UV disinfection)
Fig. 10
Condenser