Leica Cm1100 User manual

Instructions for use

Leica CM1100 V1.3 RevB English – 09/2012

Order-No.: 14 0469 80101, RevB

Always keep this manual near the instrument!

Read carefully prior to operating the instrument!

Leica CM1100

Cryostat

Leica CM1100 – Cryostat

1. Important information

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17-19

D-69226 Nussloch

Germany

Telephone: 06224/143-0

Telefax: 0 62 24/143-2 68

web: http://www.LeicaBiosystems.com

Serial No. .....................................................................

Year of manufacture: .................................................

Manufactured: ........ Federal Republic of Germany

Until the ratification of the guideline for in-vitro di-

agnostic instruments this product is categorized

according to the MedGV (National Regulations for

Medical Appliances) as a Class 3 equipment.

The information, numerical data, notes and value judg-

ments contained in this manual represent the current

state of scientific knowledge and state-of-the-art tech-

nology as we understand it following thorough investi-

gation in this field. We are under no obligation to up-

date the present manual periodically and on an ongo-

ing basis according to the latest technical develop-

ments, nor to provide our customers with additional

copies, updates etc. of this manual.

For erroneous statements, drawings, technical illus-

trations etc. contained in this manual we exclude li-

ability as far as permissible according to the national

legal system applicable in each individual case. In par-

ticular, no liability whatsoever is accepted for any fi-

nancial loss or consequential damage caused by or

related to compliance with statements or other infor-

mation in this manual.

Statements, drawings, illustrations and other informa-

tion as regards contents or technical details of the

present manual are not to be considered as warranted

characteristics of our products. These are determined

only by the contract provisions agreed between our-

selves and our customers.

Leica reserves the right to change technical specifi-

cations as well as manufacturing processes without

prior notice. Only in this way is it possible to continu-

ously improve the technology and manufacturing tech-

niques used in our products.

This document is protected under copyright laws. Any

copyrights of this document are retained by Leica

Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any

parts thereof) by means of print, photocopy, microfiche,

web cam or other methods – including any electronic

systems and media – requires express prior permis-

sion in writing by Leica Biosystems Nussloch GmbH.

For the instrument serial number and year of manu-

facture, please refer to the name plate at the back of

the instrument.

4Instructions for use V1.3 RevB – 09/2012

2. Table of contents

1. Important information ....................................................................................................................................................3

2. Table of contents ............................................................................................................................................................4

3. Safety instructions for handling the instrument........................................................................................................5

3.1 Safety features ............................................................................................................................................................................................ 5

3.1.1 Locking the handwheel .............................................................................................................................................................................. 5

3.1.2 Knife guard ................................................................................................................................................................................................... 5

3.2 General hazards .......................................................................................................................................................................................... 5

4. Technical data ................................................................................................................................................................8

5. General description .......................................................................................................................................................9

5.1 Leica CM 1100 Overview ............................................................................................................................................................................ 9

5.2 Product description .................................................................................................................................................................................. 10

5.3 Standard delivery range ........................................................................................................................................................................... 10

6. Unpacking and installation .................................................................................................................................... 11

6.1 Unpacking ................................................................................................................................................................................................... 11

6.2 Site requirements ..................................................................................................................................................................................... 12

6.3 Transport to the installation site ............................................................................................................................................................. 12

7. First use of the instrument ....................................................................................................................................... 13

7.1 Connection to mains power .................................................................................................................................................................... 13

7.2 Installing the accessories........................................................................................................................................................................ 13

7.3 Switching on .............................................................................................................................................................................................. 14

7.4 Functions of the control unit ................................................................................................................................................................... 14

7.4.1 LEDs ............................................................................................................................................................................................................. 14

7.4.2 Function keys ............................................................................................................................................................................................. 14

7.4.3 Setting user definable parameters ........................................................................................................................................................ 15

7.4.4 Starting and terminating a manual defrost cycle ............................................................................................................................... 15

7.5 Temperature selection chart (in minus °C) .......................................................................................................................................... 16

8. Daily operation ........................................................................................................................................................... 17

8.1 Specimen discs ......................................................................................................................................................................................... 17

8.1.1 Specimen freezing.................................................................................................................................................................................... 17

8.1.2 Fixing the specimen disc in the specimen head ................................................................................................................................. 17

8.2 Knife holder CE .......................................................................................................................................................................................... 18

8.2.1 Changing the rear pressure plate .......................................................................................................................................................... 18

8.2.2 Adjusting the rear pressure plate .......................................................................................................................................................... 18

8.2.3 Adjusting the front pressure plate .......................................................................................................................................................... 19

8.2.4 Inserting the blade .................................................................................................................................................................................... 19

8.2.5 Lateral displacement ................................................................................................................................................................................ 20

8.2.6 Adjusting the anti-roll plate ..................................................................................................................................................................... 20

8.2.7 Replacing the glass anti-roll plate ......................................................................................................................................................... 21

8.3 Coarse feed wheel.................................................................................................................................................................................... 22

8.4 Trimming .................................................................................................................................................................................................... 22

8.5 Sectioning .................................................................................................................................................................................................. 23

8.6 Section transfer ......................................................................................................................................................................................... 23

8.7 Defrosting ................................................................................................................................................................................................... 24

8.7.1 Programming an automatic defrost cycle ............................................................................................................................................ 24

8.7.2 Starting a manual defrost cycle ............................................................................................................................................................. 24

9. Trouble sh ooting ....................................................................................................................................................... 25

10. Cleaning and disinfection ....................................................................................................................................... 28

10.1 Cleaning...................................................................................................................................................................................................... 28

10.2 Spray disinfection with Leica Cryofect ................................................................................................................................................. 28

10.3 Removal of the microtome ...................................................................................................................................................................... 29

10.4 Reassembly of the microtome ................................................................................................................................................................ 30

11. Maintenance ............................................................................................................................................................... 31

11.1 General maintenance ............................................................................................................................................................................... 31

11.2 Fuse replacement ..................................................................................................................................................................................... 31

12. Optional accessories ............................................................................................................................................... 32

12.1 Mobile heat extractor ............................................................................................................................................................................... 32

12.2 Thermal block ............................................................................................................................................................................................ 32

13. Warranty and service ............................................................................................................................................... 33

14. Decontamination Certificate (master) ...................................................................................................................... 34

16. Information for the People´s Republic of China ................................................................................................ 35

Leica CM1100 – Cryostat

3. Safety instructions for handling the instrument

3.1 Safety features

The instrument design incorporates the following

safety features: handwheel lock, glass anti-roll guide

of the knife holder CE that has the protective function

of a knife guard.

3.1.1 Locking the handwheel

Prior to manipulating the knife holder and

specimen, or changing the specimen or knife,

and during breaks, always lock the

handwheel!

The handwheel can be locked in the upper turning

point.

Fig. 5.1

3.1.2 Knife guard

Prior to manipulating the knife holder and

specimen, or changing the specimen or knife,

and during breaks, always cover the cutting

edge with the anti-roll guide!

The knife holder CE for disposable blades has a glass

anti-roll guide which is positioned over the knife edge

while sectioning to prevent injury thus acting as a knife

guard.

3.2 General hazards

Despite the safety features provided for operator

safety, the use of the instrument and the applications

for which is designed involve certain risks that cannot

be totally eliminated:

• The handling of disposable blades involves a con-

siderable risk of injury.

• Extended skin contact with cold parts of the instru-

ment can cause frostbite!

• Sectioning infectious and/or radioactive materials

constitutes a hazard.

• Rotate the handwheel until the handle (1) is posi-

tioned in the upper turning point.

• To lock, push the lever (2) towards the housing.

• To unlock, pull the lever (2) away from the housing.

6Instructions for use V1.3 RevB – 09/2012

Connection to mains power

• Please refer to the 'Technical data'!

• The instrument must be connected to a

grounded mains power outlet socket.

• During the start-up of the compressor the

nominal voltage must not drop below the

values specified in the 'Technical data'.

Please note that the compressor requires

a start-up current between 10 and 25 A.

Therefore, the electric circuit at the instal-

lation site must be inspected by an elec-

trical engineer to ensure that it meets the

requirements for a smooth operation of the

instrument.

A constant adequate power supply to the

instrument must be ensured at all times.

Failure to comply with the above will

cause severe damage to the instrument.

• Caution:

240 V units may be operated only with the

step-up transformer supplied with the in-

strument!

Failure to comply with this will cause se-

vere damage to the instrument!

• After transporting, wait at least 4 hours be-

fore turning the instrument on.

This waiting period is necessary to allow

the compressor oil, which may have been

displaced during transport, to return to its

original position.

Failure to comply with this can cause se-

vere damage to the instrument.

3. Safety instructions for handling the instrument

Transport and installation

• The instrument must be transported in an

upright position only, or at an angle of max.

30 °!

• Do not carry or reposition the instrument

by holding it at parts of the housing or at

the handwheels! Only use the carrying

handles at the front and rear for carrying!

• Prior to transporting the instrument, pull

out the retractable handles of the hand-

wheel and coarse feed wheel and place

them in the depression provided in the

center of the wheel!

• Do not operate in rooms with explosion

hazard!

• To ensure an adequate cooling capacity,

the instrument must be set up with at least

10 cm distance from walls and furniture!

• Do not place anything next to the compres-

sor ventilation grids to ensure adequate

ventilation at all times!

Leica CM1100 – Cryostat

3. Safety instructions for handling the instrument

Sectioning

• Take care when handling disposable

blades.

The cutting edge is extremely sharp and

can cause severe injury!

• Never leave the knife holder with a blade

mounted lying around!

• Dispose of used blades by inserting them

into the receptacle provided at the bottom

of the disposable blade dispenser!

• Always clamp the specimen before the

blade!

• Take care when removing the section - the

cutting edge is exposed!

• Prior to manipulating the knife holder and

specimen, or changing the specimen or

blade, and during breaks, always lock the

handwheel and cover the cutting edge

with the anti-roll guide!

• Avoid extended skin contact with cold

parts of the instrument as this can cause

frostbite!

Cleaning and disinfection

• Do not use organic solvents or any other

aggressive substances for cleaning and

disinfection!

We strongly recommend the use of Leica

Cryofect disinfectant spray!

Only use alcohol or common disinfectants

based on alcohol!

• For spray disinfection follow the instruc-

tions for use supplied with the disinfec-

tant!

• Do not use external heaters for drying the

cryochamber. This can cause damage to

the slot cover!

Removal of the microtome

• Before removing the microtome, turn the

instrument off with the mains switch!

• Wear appropriate protective gloves to re-

move the cold microtome from the cryo-

chamber! Risk of frost bite!

Or wait until the microtome has reached

room temperature.

• The microtome must be completely dry be-

fore reinstallation. Humidity inside will

condense and freeze in the cold cryostat

and thus may cause malfunctions or dam-

age.

Maintenance

• Only qualified and authorized service per-

sonnel may access the internal compo-

nents of the instrument for service and re-

pair.

Fuse replacement

• Only use a fuse of the same specification!

For the required value, please refer to

Chapter 4. 'Technical data' or to the fuse

specification label at the rear of the instru-

ment.

8Instructions for use V1.3 RevB – 09/2012

4. Technical data

Operating temperature range: 18 °C to 35 °C.

All specifications related to temperature are valid only for an ambient temperature up to 22 °C and for an air

humidity lower than 60%!

Type CM 1100-1 CM 1100-3 CM 1100-11

Nominal voltage 230 V AC ±10% 120 V AC ±10% 100 V AC ±10%

Nominal frequency 50 Hz 60 Hz 50/60 Hz

Power draw

during refrigeration 170 VA 170 VA 170 VA

during defrosting for 10 min. 320 VA 400 VA 650 VA

Maximum start-up current for 5 sec. 10 Aeff. 25 Aeff. 25 Aeff.

Protective class I I I

Mains fuse 2-pole, T 10 A 2-pole, T 10 A 2-pole, T 10 A

Pollution degree 2 2 2

Overvoltage installation category II II II

Heat output

during refrigeration 170 J/s 170 J/s 170 J/s

during defrosting for 10 min. 320 J/s 400 J/s 650 J/s

Transformer fuse protection F3 T 4 A T 4 A T 4 A

acc. to DIN-IEC 127-II UL-listed UL-listed

Refrigeration

Temperature range 0 °C to -30 °C ±10%

at an ambient temperature of 22 °C

and air humidity _<60% when lid is closed;

adjustable in 1 K increments

Defrosting automatic hot gas defrosting,

defrost time freely programmable; 1 defrost cycle/ 24 h,

manual defrosting

duration: 20 min.

Power draw 226 W 204 W 235 W /269 W

Refrigerating output 1) 193 W 185 W 190 W

Maximum nominal pressure 15 bar 15 bar 15 bar

Safety factor 3 3 3

Refrigerant 170 g ± 5 g R134a * 170 g ± 5 g R134a * 155 g ± 5 g R134a *

Compressor oil 0.4 l alpha 22, Kyodo * 0.4 l alpha 22, Kyodo * 0.4 l alpha 22, Kyodo *

1) Rating acc. to ASHRAE: Evaporating temperature -23.3 °C

Liquefier temperature 54.4 °C

Ambient temperature 32.2 °C

Microtome

Type Rotary microtome

Section thickness setting range 0 - 20 μm, continuously adjustable

Division of the scale 2 μm

Horizontal specimen feed 15 mm ± 1 mm

Vertical stroke 46 mm ± 1 mm

Maximum specimen size 36 mm

Cryocabinet

Dimensions (W x D x H) 570 x 780 x 380 mm

Weight (including microtome) 50 kg

* Refrigerant and compressor oil must be replaced by authorized service personnel!

Leica CM1100 – Cryostat

5.1 Leica CM 1100 Overview

5. General description

Cryochamber

lid

Carrying

handles

Control unit

Handwheel

with retractable

countersunk handle

Mains switch

Fig. 9.1

Compressor

ventilation

grids

Coarse feed

wheel with

retractable

countersunk

handle

Cryochamber

Fig. 9.2

Lever

for locking the handwheel

10 Instructions for use V1.3 RevB – 09/2012

5. General description

5.2 Product description

The Leica CM1100 is a portable compact cryostat for rapid freezing and manual sec-

tioning of tissue specimens.

Two carrying handles on the front and rear of the housing make it easy for two persons

to transport the instrument. The counterbalanced handwheel is lockable in the top po-

sition of the handle.

The corrosion-proof cryochamber is easy to clean and easily accessible after remov-

ing the lid. The cryochamber temperature is selectable between 0°C and -30°C.

The stainless steel rotary microtome is virtually maintenance-free in operation. It is de-

signed for sections in a range of 3μm or 4μm up to 20μm.

All push button controls and the display are integrated in the control unit.

Important parameters such as set temperature and defrost time can be programmed.

Cryochamber defrosting takes 20 minutes. It is possible to program an automatic de-

frost cycle for a certain time of the day and to activate a manual defrost cycle when re-

quired.

5.3 Standard delivery range

Standard delivery includes:

1 Basic instrument

1 Accessory-kit consisting off:

1 Brush, fine .....................................................................................................................................................14 0183 28642

1 Leica-brush ...................................................................................................................................................14 0183 30751

1 Bottle of OCT-compound, mounting medium for cryosectioning, 125ml ............................................14 0201 08926

1 Allen key, size 4 ............................................................................................................................................14 0222 04139

1 Bottle of cryostat oil, type 407, 50 ml ......................................................................................................... 14 0336 06098

1 Replacement glass anti roll guide for CE-holder, 70mm wide .............................................................. 14 0419 33813

2 Specimen discs, 20mm ...............................................................................................................................14 0370 08636

1 Section waste tray, large............................................................................................................................ 14 0469 31779

1 Section waste tray, smal ............................................................................................................................14 0469 31780

1 Freezing shelf ...............................................................................................................................................14 0469 31782

1 Knife holder CE for low profile blades ......................................................................................................14 0504 33996

1 Pressure plate for high profile blades, 22° ..............................................................................................14 0504 32199

2 Fuses 5x 20 T 4.0A ........................................................................................................................................14 6943 04000

1 Pair of cut resistant gloves, size S ............................................................................................................14 0340 40859