Leica CM1900 User manual

Instruction Manual
Leica CM1900 – V5.3 English - 10/2006
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
Leica CM1900
Cryostat

3
Leica CM1900 – Cryostat
1. Introduction
Leica Microsystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Tel.: 0 62 24 - 143 0
Fax: 0 62 24 - 143 200
eMail: [email protected]
Internet: http://www.histo-solutions.com
The information, numerical data, notes and value judg-
ments contained in this manual represent the current
state of scientific knowledge and state-of-the-art tech-
nology as we understand it following thorough investi-
gation in this field. We are under no obligation to up-
date the present manual periodically and on an ongo-
ing basis according to the latest technical develop-
ments, nor to provide our customers with additional
copies, updates etc. of this manual.
For erroneous statements, drawings, technical illus-
trations etc. contained in this manual we exclude li-
ability as far as permissible according to the national
legal system applicable in each individual case. In par-
ticular, no liability whatsoever is accepted for any fi-
nancial loss or consequential damage caused by or
related to compliance with statements or other infor-
mation in this manual.
Statements, drawings, illustrations and other informa-
tion as regards contents or technical details of the
present manual are not to be considered as warranted
characteristics of our products. These are determined
only by the contract provisions agreed between our-
selves and our customers.
Leica reserves the right to change technical specifi-
cations as well as manufacturing processes without
prior notice. Only in this way is it possible to continu-
ously improve the technology and manufacturing tech-
niques used in our products.
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Microsystems Nussloch GmbH.
Any reproduction of text and illustrations (or of any
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sion in writing by Leica Microsystems Nussloch GmbH.
For the instrument serial number and year of manu-
facture, please refer to the name plate at the back of
the instrument.
© Leica Microsystems Nussloch GmbH

Instruction manual V 5.3 – 10/2006
4
2. Table of contents
2. Table of contents.................................................................................................................................................................... 4
3. Safety instructions for handling the instrument ............................................................................................................... 5
4. Technical data ........................................................................................................................................................................ 8
5. General description ............................................................................................................................................................. 10
5.1 Optional models .................................................................................................................................................................... 10
5.2 Designed use/improper handling ...................................................................................................................................... 10
5.3 Standard delivery ................................................................................................................................................................. 10
6. Unpacking the instrument .................................................................................................................................................. 11
6.1 How to open the crate ......................................................................................................................................................... 11
6.2 How to remove the packing materia ................................................................................................................................. 11
6.3 Mounting the ramp ............................................................................................................................................................... 12
6.4 Transport to the installation site ........................................................................................................................................ 13
7. Installation ............................................................................................................................................................................ 14
7.1 Site requirements ................................................................................................................................................................. 14
7.2 Transport to the desired site - Relocation ....................................................................................................................... 15
7.3 Assembly of the handwheel ............................................................................................................................................... 17
7.4 Locking the handwheel ....................................................................................................................................................... 17
7.5 Mounting the heat extractor .............................................................................................................................................. 17
7.6 Inserting the accessories ................................................................................................................................................... 18
7.7 Inserting the optional accessories ................................................................................................................................... 19
8. Operation ............................................................................................................................................................................... 20
8.1 Precooling the knife ............................................................................................................................................................. 20
8.2 Installing the knife holder base ......................................................................................................................................... 20
8.3 Inserting the knife holder .................................................................................................................................................... 21
8.4 Connection to the mains ..................................................................................................................................................... 21
8.5 Turning on the instrument .................................................................................................................................................. 21
8.6 Leica CM1900 – Overview ................................................................................................................................................... 22
8.7 Programming the desired values ...................................................................................................................................... 24
9. Daily operation ..................................................................................................................................................................... 30
9.1 Selection of the adecuate chamber temperature .......................................................................................................... 30
9.2 Specimen freezing ............................................................................................................................................................... 30
9.3 Activating / deactivating the specimen cooling ............................................................................................................. 31
9.4 Activating / deactivating the cryochamber cooling ...................................................................................................... 31
9.5 Inserting the specimen discs in the specimen head ..................................................................................................... 31
9.6 Inserting the knife in the knife holder ............................................................................................................................... 32
9.7 Moving the specimen towards or away from the knife via coarse feed ................................................................... 33
9.8 Trimming ................................................................................................................................................................................ 33
9.9 Adjustment of the anti-roll guide ....................................................................................................................................... 34
9.10 Sectioning .............................................................................................................................................................................. 34
10. Cleaning and disinfection .................................................................................................................................................. 35
10.1 Cleaning and disinfection ................................................................................................................................................... 35
10.2 Turning the instrument back on ......................................................................................................................................... 35
11. Removal of the microtome .................................................................................................................................................. 36
11.1 How to remove the microtome: ........................................................................................................................................ 36
12. Reinstallation of the microtome ........................................................................................................................................ 38
12.1 How to return the microtome to the cryochamber ........................................................................................................ 38
13. Maintenance ......................................................................................................................................................................... 40
13.1 General maintenance .......................................................................................................................................................... 40
13.2 Replacement of the lamp .................................................................................................................................................... 41
14. Troubleshooting ................................................................................................................................................................... 42
15. Temperature Selection Chart............................................................................................................................................. 45
16. Optional accessories .......................................................................................................................................................... 46
16.1 Orienting specimen head .................................................................................................................................................... 46
16.2 Thermal block ....................................................................................................................................................................... 47
17. Ordering information ........................................................................................................................................................... 48
18. Warranty and service .......................................................................................................................................................... 50

5
Leica CM1900 – Cryostat
Microtome knives
• Take care when handling microtome knives and
disposable blades. The cutting edge is extremely
sharp and can cause severe injury!
• Never leave knives and knife holders with a knife/
blade mounted lying around!
• Do not place a knife on a table with the cutting edge
facing upward!
• Never try to catch a falling knife!
• Prior to manipulating the knife and specimen, or
changing the specimen or knife, always lock the
handwheel and cover the cutting edge with the knife
guard!
• Always lock the handwheel and cover the cutting
edge of the knife with the knife guard during breaks!
• Avoid contact with cold parts of the instrument as
this can cause frostbite!
Unpacking and installation
• To ensure an adequate cooling capacity, the instru-
ment must be set up maintaining a minimum dis-
tance from walls and furniture (see 'Technical
data').
• The instrument must be transported in an upright
position or slightly tilted (max. 30°).
• To ensure a safe transportation with a fork lift 3
people are required: one operating the fork lift, and
the other 2 holding the instrument on either side to
prevent it from sliding down.
• Before connecting to the mains power, please
check if the local voltage complies with the power
rating specified on the name plate of the instrument
(see also 'Technical data')!
• Plug the instrument only to power sockets with
ground!
• After transporting, wait at least 4 hours before turn-
ing the instrument on.
This waiting period is necesssary to allow the com-
pressor oil , which may have been displaced dur-
ing transport, to return into its original position.
Failure to comply with this will cause severe dam-
age to the instruments!
Due to the weight of the instrument (170 kg) 4
people are required for transportation (2
people per handle).
Symbols used in this manual and their meaning
Notes
i.e. important user information appears in a
gray box and is marked by an information
symbol.
Figures in brackets refer to item numbers in
drawings or to the drawings themselves.
3. Safety instructions for handling the instrument
Warnings
appear in a gray box and are marked by a
warning triangle
(5)
(Fig.5)

Instruction manual V 5.3 – 10/2006
6
Cleaning and disinfection - Turning the
instrument back on
It is not necessary to remove the microtome
for disinfecting the cryochamber, however,
removal is possible, if required!
• When disinfecting, please take appropriate protec-
tive measures (gloves, mask, protective clothing,
etc.).
• When using detergents and disinfectants please
comply with the safety precautions of the disinfec-
tant manufacturer.
• Only use acetone for cleaning the plastic anti-roll
plate of the knife holders CN and CS. The glass anti-
roll plate of the knife holder CE can be cleaned ei-
ther with acetone or alcohol.
• Dispose of waste liquid according to the waste dis-
posal regulations!
• Do not use uncontrolled external heaters for drying
the cryochamber. This can cause damage to the
cooling system!
• Do not turn the instrument on before the
cryochamber is completely dry!
Frost formation!
• Dry all parts completely before reinserting them in
the cryochamber!
• The front panel and the slit cover of the microtome
must be completely dry before turning on the in-
strument!
3. Safety instructions for handling the instrument
Removal/Reinstallation of the microtome
• Prior to removing the microtome, turn the instru-
ment off with the mains switch and pull the mains
plug!
• Remove sliding window before removing the micro-
tome!
• Before removing the microtome, lock the
handwheel in the lowest position. When removing
the microtome, the specimen head will rapidly fall
down and might injure the operator's hands!
• The microtome must be entirely dry before reinstal-
lation. Humidity inside will condense and freeze,
causing malfunctions and damage!
• All components removed from the cryostat must be
carefully dried before returning them to the
cryochamber!
• The cooling chamber must be entirely dry when
turning on the instrument (Frost formation)!
• When removing the microtome for cleaning or dis-
infection, please keep in mind the safety instruc-
tions in chapter 10 'Cleaning and Disinfection'.

7
Leica CM1900 – Cryostat
Handling samples - Defrosting
• When working with possibly contaminated or in-
fected material the general safety guidelines for
laboratories must be applied!
• Before defrosting the cryochamber remove all
samples!
• Before defrosting the specimen head remove all
samples!
• Never leave samples in the cryochamber! - The in-
strument is not made for storing frozen specimen!
3. Safety instructions for handling the instrument
Maintenance
• Turn the instrument off with the mains switch and
pull the mains plug, before replacing the fuses!
• Only use fuses of the same specification ! For the
required values refer to chapter 4 'Technical Data'!
• Turn the instrument off with the mains switch and
pull the mains plug before replacing the lamp!
• If the lamp is broken, it must be replaced by the tech-
nical service, as the replacement involves a high
risk of injury!
• Only use lamps of the same specification! For the
required type refer to chapter 4 'Technical Data'!

Instruction manual V 5.3 – 10/2006
8
Type
Nominal voltage
Nominal frequency
Power draw
Max. start-up current for 5 sec
Protective class
Mains fuses (MDA by Bussmann)
Automatic fuse
Pollution degree ➁
Overvoltage installation category
Heat emission (max.)
Refrigeration CM1900, 50 Hz CM1900, 60 Hz
Cryochamber
Temperature range 0 - -35 °C ± 3 K, at an ambient 0 - -35 °C ± 3 K, at an ambient
temperature of 22 °C temperature of 22 °C
Refrigeration capacity ➀690 W 690 W
Cut-off pressure 25 bar 25 bar
Safety factor 3 3
Refrigerant* 275 g (± 5 g) refrigerant R 404A* 265 g (± 5 g) refrigerant R 404A*
Compressor oil* 0,6 l EMKARATE RL22S, ICI* 0,6 l EMKARATE RL22S, ICI*
Defrosting of cryochamber
Automatic defrosting
Programmable: yes yes
Defrosting intervals 1 defrost cycle/ 24 hours 1 defrost cycle/ 24 hours
Defrosting period: 9 minutes 9 minutes
Automatic stop of manual defrosting: at -5 °C at -5 °C
Manual defrosting
Defrosting period: 9 minutes 9 minutes
Automatic stop of manual defrosting: at -5 °C at -5 °C
Quick freeze shelf
Max. temperature: - 43 °C (+ 0 K / - 2 K) - 43 °C (+ 0 K / - 2 K)
Number of quick freeze stations: 10 10
Specimen cooling
Temperature range -10 - -50 °C ± 2 K, at an ambient -10 - -50 °C ± 2 K, at an ambient
temperature of 22 °C temperature of 22 °C
Refrigeration capacity ➀320 W 320 W
Cut-off pressure 25 bar 25 bar
Safety factor 3 3
Refrigerant* 210 g (± 5 g) refrigerant R404A* 210 g (± 5 g) refrigerant R404A*
Compressor oil* 0,4 l alpha 22, Kyodo* 0,4 l alpha 22, Kyodo*
Defrosting of specimen head
Automatic defrosting no no
Manual defrosting
Defrosting period: 10 minutes 10 minutes
Automatic stop of manual defrosting: 10 min. after reaching +20 °C 10 min. after reaching +20 °C
4. Technical data
➀acc. to CECOMAF: liquid temperature 45 °C, evaporation temperature: -25°C ➁according to IEC-1010; UL 3101
-1 -2 -3 -4 -5 -6
230 V AC 120 V AC 230 V AC 240 V AC 100 V AC 100 V AC
50 Hz 60 Hz 60 Hz 50 Hz 60 Hz 50 Hz
1800 VA 1800 VA 1800 VA 1800 VA 1800 VA 1800 VA
25 A eff. 35 A eff. 25 A eff. 25 A eff. 30 A eff. 35 A eff.
IIIIII
T8A - T8A T8A - -
T10A T1 T15A T1 T10A T1 T10A T1 T15A M3 T15A M3
222222
II II II II II II
1800 J/s 1800 J/s 1800 J/s 1800 J/s 1800 J/s 1800 J/s

9
Leica CM1900 – Cryostat
Microtome
Type Microtome encapsulated in the cryochamber
Section thickness setting 0 - 60 μm, infinitely variable
Specimen feed 25 mm
Knife holder repositioning on the microtome base plate 30 mm
Vertical stroke 59 mm
Specimen retraction yes (US optional)
Maximum specimen size Ø 40 mm
Cryostat
Dimensions
Width (w/o handwheel) 790 mm
Width (incl. handwheel) 890 mm
Depth (cabinet only) 725 mm
Depth (cabinet incl. mains plug) 800 mm
Overall height 1200 mm
Working height 1000 mm
Weight (instrument only) 168 kg
Weight incl. accessories 170 kg
Set-up conditions
Distance to walls and furniture;
calculated from the cabinet rear: 10 cm
right side: 20 cm
left side: 10 cm
Plug the instrument only to power sockets with ground.
Mains cable length: – 3,5 m
Extendable: no
The installation site must be free of draft and insolation; the floor must be free from vibrations.
Lamp
50 Hz-Version: Osram DULUX S 11 W/21
60 Hz-Version: Osram DULUX S 13 W/21
*) Refrigerant and compressor oil must only
be replaced by authorized service personnel!
4. Technical data
Conditions
ambient temperature: 8°C to 40 °C
Operating temperature range: 0°C to -35°C
Temperature range during storage: + 5°C to +55°C
Relative humidity: max. 60%, non-condensing
Humidity during storage: < 60%

Instruction manual V 5.3 – 10/2006
10
5. General description
5.3 Standard delivery
1 Basic instrument
1 Handwheel, assy. ..................................................................................................... 0416 18478
1 Heat extractor, stationary ....................................................................................... 0452 27918
1 Low-temperature stabilizer for heat extractor .................................................... 0452 27919
1 Set of specimen discs ............................................................................................. 0470 43550
- 4 specimen discs, 25 mm ................................................................................... 0416 19275
- 4 specimen discs, 30 mm ................................................................................... 0370 08587
1 One-piece storage shelf .......................................................................................... 0452 28594
1 Freezing shelf cover ................................................................................................. 0452 28624
1 Brush shelf with holder ........................................................................................... 0452 28799
1 Section waste tray ................................................................................................... 0452 28595
1 toolkit: ......................................................................................................................... 0436 43463
- 1brush, fine .......................................................................................................... 0183 28642
- 1 Leica-brush ....................................................................................................... 0183 30751
- 1 Allen key, no. 1.5 .............................................................................................. 0222 10050
- 1 Allen key, no. 2.5 .............................................................................................. 0222 04137
- 1 Allen key, no. 3.0 .............................................................................................. 0222 04138
- 1 Allen key, no. 4.0 .............................................................................................. 0222 04139
- 1 Allen key with spherical head, no. 4.0 ......................................................... 0222 32131
- 1 Allen key, no. 5 ................................................................................................. 0222 04140
- 1 Allen key with handle, no. 5............................................................................ 0194 04760
- 1 Allen key, no. 6 ................................................................................................. 0022 04141
- 1 Single-head wrench, no. 13 ........................................................................... 0330 33149
- 1 Single-head wrench, no. 16 ........................................................................... 0330 18595
1 Bottle of OCT-Compound, mounting med. f. cryosectioning, 125 ml ................ 0201 08926
1 Bottle of cryostat oil, type 407, 50 ml ..................................................................... 0336 06089
1 Storage mat ............................................................................................................... 0452 27913
1 Pair of safety gloves for cryosectioning, size S .................................................. 0340 40859
1 Instruction manual Leica CM1900 - G/E/F/S ......................................................... 0708 37105
5.1 Designed use/improper handling
The rapid sectioning cryostat CM1900 is provided with
a fully encapsulated microtome with an independent
specimen cooling system.
The cryostat CM1900 is appropriate for InVitro
Diagnostic (IVD) applications.
The instrument may only be operated within the scope
of its designated use as described above and as per
the instructions given in this manual.
The instrument is CFC-free and is designed and
manufactured according to VDE and UL regulations.
Samples must not be stored unattended in the cryostat
for a long period since they could be destroyed in case
of a power failure or malfunction of the instrument.
The cryochamber temperature can rise considerably
during defrosting. Heat-sensitive samples must there-
fore be protected from exposure to elevated tempera-
tures and removed prior to defrosting.
The instrument may only be used for the specified ap-
plication and operated in accordance with the instruc-
tions given in this manual.
Any other use of this instrument is considered as im-
proper operation!
5.2 Optional models
The following models are available:
• CM1900 with specimen retraction
• CM1900 without specimen retraction
Please, indicate type when ordering!