Leica CM1950 User manual

Operating Manual
Leica CM1950, RevC, English, 1v3 – 04/2010
Always keep this manual near the instrument.
Read carefully prior to working with the instrument!
/HLFD&0
Cryostat

3
Leica CM1950 – Cryostat
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
eMail: h[email protected]
Internet: http://www.leica-microsystems.com
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical spec-
ifications as well as manufacturing processes
without prior notice. Only in this way is it possible
to continuously improve the technology and man-
ufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods—includ-
ing any electronic systems and media—requires
express prior permission in writing by Leica Bio-
systems Nussloch GmbH.
For the instrument serial number and year of man-
ufacture, please refer to the name plate at the
back of the instrument.
© Leica Biosystems Nussloch GmbH
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-
of-the-art technology as we understand it follow-
ing thorough investigation in this field. We are
under no obligation to update the present manu-
al periodically and on an ongoing basis accord-
ing to the latest technical developments, nor to
provide our customers with additional copies,
updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we
exclude liability as far as permissible according
to the national legal system applicable in each
individual case. In particular, no liability whatso-
ever is accepted for any financial loss or conse-
quential damage caused by or related to compli-
ance with statements or other information in this
manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of our
products.
1. NOTE

4Operating Manual, Rev C, 1v3 – 04/2010
Table of contents
1. Important Information ................................................................................................................ 6
1.1 Symbols used in this manual and their meaning .................................................................... 6
1.2 Qualification of personnel .......................................................................................................... 6
1.3 Designated use ............................................................................................................................ 6
1.4 Instrument type ............................................................................................................................ 6
2. Safety and Design ...................................................................................................................... 7
2.1 General safety instructions........................................................................................................ 7
2.2 Unpacking and installation ........................................................................................................ 7
2.3 Safety features .......................................................................................................................... 10
2.4 Securing/locking the handwheel ............................................................................................ 11
2.5 Cleaning, disinfection – Turning the instrument back on ................................................... 12
2.6 Handling specimens – Defrosting ........................................................................................... 13
2.7 Removal of the microtome ....................................................................................................... 13
2.8 Maintenance .............................................................................................................................. 13
3. Technical Data ......................................................................................................................... 14
4. Standard Delivery..................................................................................................................... 16
5. General Overview .................................................................................................................... 18
5.1 Control panel fields and cryostat chamber ........................................................................... 19
6. Installation................................................................................................................................. 20
6.1 Site requirements ...................................................................................................................... 20
6.2 Transport to the site .................................................................................................................. 21
6.3 Assembling the handwheel .....................................................................................................22
6.3.1 Locking/unlocking the handwheel.......................................................................................... 23
6.3.2 Installing the footswitch dummy (instruments with cutting motor)................................... 23
6.4 Connection to power supply system ...................................................................................... 24
6.5 Installing accessories/inserting chamber accessories ...................................................... 25
6.5.1 Installing the adjustable footrest (optional) .......................................................................... 25
6.5.2 Installing the storage systems (optional) .............................................................................. 26
6.5.3 Shelf, movable (optional).......................................................................................................... 26
6.5.4 Inserting the section waste tray ............................................................................................. 26
6.5.5 Installing the heat extractor, stationary (optional) .............................................................. 27
6.5.6 Installing the knife/blade holder and adjusting the clearance angle ............................... 27
6.5.7 Inserting/changing the bacteria filter .................................................................................... 28
6.5.8 Assembling the filter bag ......................................................................................................... 28
6.5.9 Installing the section extraction (optional) - Use with Blade Holder CE only .................. 29

5
Leica CM1950 – Cryostat
Table of contents
7. Instrument Controls ................................................................................................................. 30
7.1 Control panel fields on the CM1950 – Control panel field 1 ................................................ 30
Control panel field 2 – Electric coarse feed, sectioning and trimming thickness display ..... 32
Control panel 3 – Motorized sectioning (optional) ............................................................... 34
8. Working with the Instrument ................................................................................................. 36
8.1 Preparing cutting tools, specimen discs and preparation aids ......................................... 36
8.2 Turning the instrument on ........................................................................................................ 36
8.3 Configuring the parameters ..................................................................................................... 37
8.4 Working with the precooled cryostat .................................................................................... 42
8.4.1 Preparatory work ...................................................................................................................... 42
8.4.2 Trimming with extraction – Anti-roll guide installed............................................................ 43
8.4.3 Cutting with extraction – Anti-roll guide installed................................................................ 45
9. Troubleshooting ....................................................................................................................... 48
9.1 Problems during work............................................................................................................... 48
10. Temperature Selection Chart (in minus °C) ........................................................................ 52
11. Optional Accessories .............................................................................................................. 53
11.1 Ordering information ................................................................................................................. 53
12. Maintenance and Cleaning .................................................................................................... 68
12.1 General maintenance ............................................................................................................... 68
12.2 Changing fuses .......................................................................................................................... 70
12.3 Replacing the UVC lamp ........................................................................................................... 70
12.3.1 Replacing the fluorescent lamp .............................................................................................. 72
13. Decontamination Certificate (master) ..................................................................................................... 73
14. Warranty and Service ............................................................................................................. 75
Peoples Republic of China ..................................................................................................... 76

6Operating Manual, Rev C, 1v3 – 04/2010
1. Important Information
1.1 Symbols used in this manual and
their meaning
Notes
i.e. important user information appear
in a gray box and are marked by the
symbol .
Caution – UVC radiation!
Figures in brackets refer to item num-
bers in drawings or to the drawings
themselves.
(5)
(Fig.5)
Warnings
appear in a gray box and are marked
by a warning triangle .
1.4 Instrument type
All information given in this instruction manual
applies only to the instrument type indicated on
the title page. A name plate, indicating the instru-
ment serial
number, is at-
tached to the
back of the
instrument.
Fig. 1
1.3 Designated use
The CM1950 is a high-performance cryostat with
an encapsulated microtome and separate speci-
men cooling. It features a UV disinfection system,
an (optional) integrated extraction system for sec-
tion waste and an (optional) motor for motorized
sectioning.
The cryostat is designed to produce frozen
sections for biological, medical and industrial
applications.
The CM1950 is suitable for in-vitro diagnostic
(IVD) applications.
The instrument may only be operated within the
scope of its designated use as described above
and as per the instructions given in this manual.
Any other use of this instrument is considered
as improper operation.
Despite chemical and/or UV-light
disinfection, personal safety precau-
tions as per the applicable laboratory
regulations (i.e safety goggles,
gloves, laboratory coat and mask
must be worn).
This type of disinfection reduces the
number of germs by at least 99.99%.
1.2 Qualification of personnel
The Leica CM1950 may be operated by trained
laboratory personnel only.
All laboratory personnel designated to operate
the instrument must carefully read the present
instruction manual prior to starting work with the
instrument.

7
Leica CM1950 – Cryostat
2.1 General safety instructions
The CM1950 is a cryostat with an encapsulated microtome and separate spec-
imen cooling. It is primarily used for work in the area of fast-cut diagnostics.
The displays and instrument controls are easy to operate due to their largely
self-explanatory symbols. LED displays make it easy to read. The cryochamber
is made of seamlessly welded, high-quality stainless steel that is free of difficult-
to-access corners and thus easy to clean and disinfect.
This instrument has been built and tested in accordance with the following safety
regulations on electrical measuring, control, regulating and laboratory devices.
In order to maintain this condition and to ensure safe operation, the operator
must observe the instructions and warnings contained in this instruction manual.
For current information about applicable standards, please refer to the
CE declaration of conformity on our Internet site:
www.leica-microsystems.com
2.2 Unpacking and installation
• To ensure proper function of the instrument, it must be set up with a minimum
distance on all sides from walls and furniture (see 'Site Requirements',
p. 20)
• The instrument may only be transported in an upright or slightly inclined
position.
• To ensure a safe transport with a fork lift 3 people are required: one operating
the fork lift, and the other 2 holding the instrument on either side to prevent
it from sliding down.
• Before connecting to the power supply system, please check if the local
voltage complies with the power rating specified on the name plate of the
instrument (see also 'Technical data')!
• Never connect the instrument to a power socket that does not have a
protective conductor terminal.
Length of power cable: up to 3.5 m extension possible: no
After transporting, wait at least 4 hours before turning the instrument on. This waiting period is
necessary to allow the compressor oil, which may have been displaced during transport, to return
into its original position. Any condensation on electrical parts that has formed due to tempera-
ture differences during transport must be allowed to dry completely. Failure to comply with this
can cause severe damage to the instrument!
2. Safety and Design

8Operating Manual, Rev C, 1v3– 04/2010
2. Safety and Design
Opening the packaging
1. Remove the 3 screws (1) on each of the two sides of the
box (7) at the top.
2. Remove the 2 screws on each of the 4 hinges (2) on the
front and rear side of the box.
Do NOT remove the screws of the hinges on the top
lid!
3. Remove 1 screw (3) between the two hinges on the front
and rear side of the box.
4. Remove the 3 screws (4) at the bottom on all four sides
of the box.
5. Of the two vertical rows of screws (5), remove one on
either side (which side does not matter) entirely.
22
77
77
7
13
When the instrument is delivered, check the tilt indicators on the
packaging.
If the arrowhead is blue, the shipment was transported laying flat,
was tilted at too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment for
possible damage.
Fig. 2
4
5
5
4
8
8
8
8
8
17
9
9
Cover = ramp
10
14
11
11
Fig. 3

9
Leica CM1950 – Cryostat
2. Safety and Design
Removing the packaging
1. Remove the cover (17) and place it on the floor with the foam parts facing
downwards. - The cover will be used as a ramp later.
2. Remove the 4 foam parts (6) by pulling them upwards.
3. Open the box (7) on its side hinges like an accordion partition and remove
it.
4. Remove the accessories carton (8) and operating manual on the front of
the instrument.
5. Pull off the dust cover (9) by pulling upwards.
6. Take off the foam strip (10) on the bottom rear side.
Ramp assembly
1. After the foam strip (10) is removed, 2 screws (12) are visible on the
baseplate.
2. Swing out the cover hinges (2) at the front and rear.
3. Attach the cover (17) from the rear to the transport pallet (11).
The notches (13) of the hinges (2) must be pointing towards the instrument.
4. Move the cover towards the left so that the notches (13) of the hinges
slide beneath the heads of the screws (12).
Transport to the Installation Location
The instrument must be transported in an upright position.
The cover (17) now forms a ramp on which the instrument can be rolled
down off the transport pallet.
Do not push the device by its hood (16)! Use the transport grip
points (15 )!
The rollers (14) must run over the cover hinges (2) at the front and
rear. Danger of tipping!
1. Carefully roll the instrument backwards over the ramp from the pallet.
2. Push the instrument to the installation location on the castors (14).
13
12
6
66
6
17
16
14
22
Fig. 5
Fig. 4
Fig. 6
15

10 Operating Manual, Rev C, 1v3 – 04/2010
To prevent adverse health effects from UV radiation, the UV disinfec-
tion cycle can be started only after the sliding window has been prop-
erly closed. Closing the window activates the corresponding safety
features.
The consistent use of these safety features and strict observation of the warn-
ings and cautions in this manual, will safeguard the operator from accidents
and/or personal injury to a great extent.
Microtome knives
• Take care when handling microtome knives/disposable blades. The
cutting edge is extremely sharp and can cause severe injury!
• Never leave knives and dismounted knife holders with a knife/blade
mounted lying around!
• Do not place a knife on a table with the cutting edge facing upwards!
We strongly recommend using the safety gloves included with the
standard delivery!
•Never try to catch a falling knife!
• Prior to making modifications to the knife and specimen or changing the
specimen, always lock the handwheel and cover the cutting edge with
the knife guard!
• Avoid contact with cold parts of the instrument as this can cause frostbite
– wear the safety gloves supplied!
2. Safety and Design
2.3 Safety features
The instruction manual includes important information related to the operating
safety and maintenance of the instrument
The Operating Manual is an important part of the product, and must be read
carefully before startup and use and must always be kept near the instrument.
If additional requirements on accident prevention and environmental protection
apply in the country of operation, this Operating Manual must be supplemented
by appropriate instructions to ensure compliance with such requirements.
The instrument is equipped with the following safety devices: an emergency
stop switch (motorized instruments only), a handwheel lock and centering sys-
tem (motorized instruments only), knife guard on the blade and knife holder,
and a blade ejector.