Agilent Technologies Infinity II SFC Binary 1260 User manual

Agilent Technologies
Agilent InfinityLab LC Series
1260 Infinity II SFC Binary
Pump
User Manual

1260 SFC Binary Pump
Notices
© Agilent Technologies, Inc. 2017
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Manual Part Number
G4782-90000
Edition
02/2017
Printed in Germany
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1260 SFC Binary Pump 3
In This Guide...
In This Guide...
This manual covers the Agilent InfinityLab LC Series 1260 Infinity II SFC
Binary Pump (G4782A).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information about site requirements and specifications
for the binary pump.
3 Using the Pump
This chapter explains the operational parameters of the Binary Pump.
4 Optimizing Performance
This chapter gives information on how to optimize the performance of the
Binary Pump under special operational conditions.
5 Troubleshooting and Diagnostics
Overview of the troubleshooting and diagnostic features.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Test Functions and Calibration
This chapter explains all test functions that are available for the binary pump.
8 Maintenance
This chapter describes the maintenance and repair of the module.

41260 SFC Binary Pump
In This Guide...
9 Parts and Materials for Maintenance
This chapter lists all parts and tools that are required for maintenance and
simple repairs.
10 Identifying Cables
This chapter provides information on cables used with the Agilent InfinityLab
LC Series modules.
11 Hardware Information
This chapter provides detailed technical information about your binary pump.
12 LAN Configuration
This chapter provides information on connecting the module to the Agilent
ChemStation PC.
13 Appendix
This chapter provides addition information on safety, legal and web.

1260 SFC Binary Pump 5
Contents
Contents
1 Introduction 9
Instrument and Operation 10
Overview of the Hydraulic Path 13
Leak and Waste Handling 17
2 Site Requirements and Specifications 23
Site Requirements 24
Physical Specifications 27
Performance Specifications 28
3 Using the Pump 31
Magnets 32
Turn on/off 33
Status Indicators 34
Best Practices 35
Leak and Waste Handling 37
Hints for Successful Use of the Binary Pump 39
Setting up the Pump with the G4208A Instant Pilot 40
Setting up the Pump with the Instrument Control Interface 41
Solvent Information 46
Algae Growth in HPLC Systems 52
Prevent Blocking of Solvent Filters 53
Normal Phase Applications 55
4 Optimizing Performance 57
When to Use a Vacuum Degasser 58
When to Use the Active Seal Wash Option 59
How to Optimize the Compressibility Compensation Setting 60
5 Troubleshooting and Diagnostics 63
Overview of the Module’s Indicators and Test Functions 64
Agilent Lab Advisor Software 65

61260 SFC Binary Pump
Contents
6 Error Information 67
What Are Error Messages 69
General Error Messages 70
Module Error Messages 77
7 Test Functions and Calibration 89
Pump Leak Rate Test 90
8 Maintenance 91
Introduction to Maintenance 92
Cautions and Warnings 93
Cleaning the Module 95
Overview of Maintenance and Simple Repairs 96
Maintenance Procedures 97
Remove and Install Doors 98
Exchange the Purge Valve Frit or the Purge Valve 100
Replace the O-Ring on the Purge Valve 102
Remove the Pump Head Assembly 105
Maintenance of a Pump Head without Seal Wash 107
Maintenance of a Pump Head with Seal Wash 110
Reinstall the Pump Head Assembly 114
Seal Wear-in Procedure Channel B 115
Exchange the Seal Wash Cartridge 116
Replace Leak Handling System Parts 118
Exchange the Inlet Valve 119
Exchange the Outlet Valve 120
Exchange the Solvent Selection Valve 122
Replacing Module Firmware 124
9 Parts and Materials for Maintenance 125
Hydraulic Path with Solvent Selection Valve 126
Pump Head Assembly Channel B 128
Pump Head Assembly Channel A 130
Outlet Valve 132
Purge Valve Assembly 133
HPLC System Tool Kit 134
Active Seal Wash Option 135

1260 SFC Binary Pump 7
Contents
Solvent Cabinet 136
Bottle Head Assembly 137
Cover Parts 138
Accessory Kit 139
10 Identifying Cables 141
Cable Overview 142
Analog Cables 144
Remote Cables 146
CAN/LAN Cables 150
RS-232 Cable Kit 151
Agilent 1200 Module to Printer 152
11 Hardware Information 153
Firmware Description 154
Electrical Connections 157
Interfaces 159
Setting the 6-bit Configuration Switch 166
Early Maintenance Feedback 170
Instrument Layout 171
12 LAN Configuration 173
What You Have to Do First 174
TCP/IP parameter configuration 175
Configuration Switches 176
Initialization Mode Selection 177
Dynamic Host Configuration Protocol (DHCP) 179
Manual Configuration 182
13 Appendix 187
General Safety Information 188
Waste Electrical and Electronic Equipment Directive 194
Radio Interference 195
Sound Emission 196
Agilent Technologies on the Internet 197

81260 SFC Binary Pump
Contents

9
1260 SFC Binary Pump
Agilent Technologies
1
Introduction
Instrument and Operation 10
Introduction to the Pump 10
Principle of Operation 11
Product Description 12
Features 12
Overview of the Hydraulic Path 13
Leak and Waste Handling 17
Waste Concept 21
This chapter gives an introduction to the module and an instrument overview.

10 1260 SFC Binary Pump
1 Introduction
Instrument and Operation
Instrument and Operation
Introduction to the Pump
The binary pump comprises two identical pumps integrated into one housing.
Binary gradients are created by high-pressure mixing. A built-in degasser is
available for applications that require best flow stability, especially at low flow
rates, for maximum detector sensitivity. The pump is capable of delivering
flow in the range of 0.1 – 5 mL/min against up to 600 bar. A solvent selection
valve allows to form binary mixtures (isocratic or gradient). Active seal wash
is available for up to three modifier solvents on channel B, while channel A is
always the CO
2
delivering channel.
Figure 1 Overview of the pump

1260 SFC Binary Pump 11
Introduction
1
Instrument and Operation
Principle of Operation
The binary pump is based on a two-channel, dual-piston in-series design
which comprises all essential functions that a solvent delivery system has to
fulfill. Metering of solvent and delivery to the high-pressure side are
performed by two pump assemblies which can generate pressure up to
600 bar.
Each channel comprises a pump assembly including pump drive, pump head,
and outlet valve. The two channels are fed into cross connector which is
connected to a damping unit and a mixer. A pressure sensor monitors the
pump pressure. A purge valve with integrated PTFE frit is fitted to the Pump
Head B outlet for convenient priming of the modifier channel.
For pump specifications, see “Performance Specifications” on page 28.
Damper with
Mixer
Pump head B
Inlet valve
Pump head A
SSV
Purge valve
Seal wash pump
Outlet valve
Inlet valve
Pressure sensor
to autosampler

12 1260 SFC Binary Pump
1 Introduction
Instrument and Operation
Product Description
The 1260 Infinity II SFC Binary pump is equipped with passive inlet valves
and with special seals and valves to allow for CO
2
pumping in channel A while
channel B adds organic modifier for either isocratic or gradient performance.
Pump head B is also equipped with a purge valve to allow for quick changeover
of the organic modifier. In addition, it has an integrated solvent selection
2-channel degasser and built-in active seal-wash on channel A for increased
uptime
Figure 2 Overview of the pump.
Features
•Fast and precise gradients - the pump is the perfect choice for fast and
precise gradients using UV-only, as well as SFC/MS systems.
•Power range combining high pressure up to 600 bar and flow rates up to
5 mL/min for maximum SFC performance.
•Integrated 2-channel degasser change-over of solvents for purging and
priming the pump.
•Built-in active seal-wash for increased uptime.
•Integrated Solvent Selection Valve.
Status indicator
Purge valve
Degasser
Pump head A
Pump head B
Leak drain
Power switch

1260 SFC Binary Pump 13
Introduction
1
Overview of the Hydraulic Path
Overview of the Hydraulic Path
The solvent from the bottle in the solvent cabinet enters the pump through an
active inlet valve. Each side of the binary pump comprises two substantially
identical pump units. Both pump units comprise a ball-screw drive and a
pump head with two sapphire pistons for reciprocating movement.
Figure 3 Pump head
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YDOYH
,QOHW
YDOYH
)URPVROYHQWERWWOH
7RPL[LQJFKDPEHU
6HDO
3LVWRQ
%DOOVFUHZGULYH
0RWRUZLWKHQFRGHU
*HDU

14 1260 SFC Binary Pump
1 Introduction
Overview of the Hydraulic Path
A servo-controlled variable reluctance motor drives the two ball-screw drives
in opposite directions. The gears for the ball-screw drives have different
circumferences (ratio 2:1) allowing the first piston to move at double the speed
of the second piston. The solvent enters the pump heads close to the bottom
limit and leaves it at its top. The outer diameter of the piston is smaller than
the inner diameter of the pump-head chamber allowing the solvent to fill the
gap in between. The first piston has a stroke volume in the range of 20 L to
100 L depending on the flow rate. The microprocessor controls all flow rates
in a range of 1 L/min to 5 mL/min. The inlet of the first pumping unit is
connected to the active inlet valve which is processor-controlled opened or
closed allowing solvent to be drawn into the first pump unit.
When turned on, the pump runs through an initialization procedure to
determine the upper dead center of the first piston of both pump channels.
The first piston moves slowly upwards to the mechanical stop of the pump
head and from there it moves back a predetermined path length. The
controller stores this piston position in memory. After this initialization the
pump starts operation with the set parameters for the two pump channels.
The inlet valve is opened and the down moving piston draws solvent into the
first pump head. At the same time the second piston is moving upwards
delivering into the system. After a controller defined stroke length (depending
on the flow rate) the drive motors are stopped and the inlet valve is closed.
The motor direction is reversed and moves the first piston up until it reaches
the stored upper limit and at the same time moving the second piston
downwards.
Then the sequence starts again moving the pistons up and down between the
two limits. During the delivery stroke of the first piston the solvent in the
pump head is pressed through the outlet valve into the second pumping unit.
The second piston draws in half of the volume displaced by the first piston and
the remaining half volume is directly delivered into the system. During the
drawing stroke of the first piston, the second piston delivers the drawn
volume into the system.
What is Solvent Compressibility Compensation?
Although the compressibility of liquids is orders of magnitude lower than the
compressibility of gases, without correction a noticeable volume error would
be seen if typical chromatographic solvents are compressed to operating
pressures as high as 600 bar. In addition, the compressibility depends on
pressure, temperature and the amount of dissolved gas. In order to minimize

1260 SFC Binary Pump 15
Introduction
1
Overview of the Hydraulic Path
the influence of the latter, the use of a vacuum degasser is mandatory for a
high flow and composition precision. Unfortunately, the influence of the
temperature on compressibility is non-linear and cannot be calculated.
The Agilent 1260 Infinity II Binary Pump features a multi point
compressibility calibration. The compressibility of a solvent is determined at
different pressures from 0 – 600 bar and stored in an XML file. This file can be
distributed to other pumps because the solvent compressibility is independent
from the pump.
The binary pump and ChemStation come with predetermined solvent
compressibility data for the most common HPLC solvents like water,
acetonitrile, methanol, etc. Users can calibrate their own solvent mixtures
with the help of an easy to use calibration procedure in the Agilent Lab
Advisor software.
Let us use the practical example from the last section once again to
understand how compressibility compensation works:
Piston 1 draws solvent at ambient pressure. The movement direction is
reversed and piston 1 now compresses the solvent until the operating pressure
of the HPLC system is reached. The outlet valve opens, and solvent is pumped
by piston 1 into pump chamber 2.
Without any compensation, the delivered volume at operating pressure would
be too low. In addition, it would take a noticeable amount of time to
recompress the solvent to operating pressure. During this time frame, no
solvent would be delivered into the system and as a result a high pressure
fluctuation (known as pressure ripple) would be observed.
When both solvent compressibility at the current operating pressure and
pump elasticity are known, the pump can automatically correct for the missing
volume by drawing the appropriate larger solvent volume at ambient pressure
and speed up the piston during the recompression phase in the first pump
chamber. As a result, the pump delivers the accurate volume with any
(calibrated) solvent at any pressure at a greatly reduced pressure ripple. For
applications that require lowest transition volume of the pump, damper and
mixer can be bypassed.

16 1260 SFC Binary Pump
1 Introduction
Overview of the Hydraulic Path
How Does Variable Stroke Volume Work?
The smaller the solvent volume in the pump chamber is, the faster it can be
recompressed to operating pressure. The binary pump allows to manually or
automatically adjust the pump stroke volume of the first piston in the range of
20 – 100 L. Due to the compression of the solvent volume in the first pump
chamber, each piston stroke of the pump will generate a small pressure
pulsation, influencing the flow ripple of the pump. The amplitude of the
pressure pulsation mainly depends on the stroke volume and the
compressibility compensation for the solvent in use. Small stroke volumes
generate less pressure pulsation than larger stroke volumes at the same flow
rate. In addition, the frequency of the pressure pulsation will be higher. This
will decrease the influence of flow pulsations on retention times.
In gradient mode, a smaller stroke volume results in less flow ripple and
reduces the composition ripple.
The binary pump uses a processor-controlled ball screw system for driving its
pistons. The normal stroke volume is optimized for the selected flow rate.
Small flow rates use a small stroke volume while higher flow rates use a higher
stroke volume.
The stroke volume for the pump is by default set to AUTO mode. This means
that the stroke is optimized for the flow rate in use. A change to larger stroke
volumes is possible but not recommended.

1260 SFC Binary Pump 17
Introduction
1
Leak and Waste Handling
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should
not exceed 2 L. For details, see the usage guideline for the Agilent 1200
Infinity Series Solvent Cabinets (a printed copy of the guideline has been
shipped with the solvent cabinet, electronic copies are available on the
Internet).
All leak plane outlets are situated in a consistent position so that all Infinity
and Infinity II modules can be stacked on top of each other. Waste tubes are
guided through a channel on the right hand side of the instrument, keeping the
front access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler (condensate)
•from the Seal Wash Sensor
•from the pump's Purge Valve or Multipurpose Valve

18 1260 SFC Binary Pump
1 Introduction
Leak and Waste Handling
Figure 4 Infinity II Leak Waste Concept (flexible rack installation)

1260 SFC Binary Pump 19
Introduction
1
Leak and Waste Handling
Figure 5 Infinity II Single Stack Leak Waste Concept (bench installation)

20 1260 SFC Binary Pump
1 Introduction
Leak and Waste Handling
Figure 6 Infinity II Two Stack Leak Waste Concept (bench installation)
The waste tube connected to the leak pan outlet on each of the bottom
instruments guides the solvent to a suitable waste container.