Biotage SPE Dry 96 User manual

Biotage®SPE Dry 96 and
Biotage®SPE Dry 96 Dual
User Manual

SPE Dry 96 and SPE Dry 96 Dual User Manual | © Biotage 2017
Contents
Biotage®SPE Dry  and
Biotage®SPE Dry  Dual
User Manual
CONTENTS
Introduction
System Precautions and Warranty
2 General Precautions
2 New System Inspection
2 Warranty and Liability
Installation
3 Connecting the Gas Supply
3 Connecting the Electrical Supply
Operation
4 Plate Clearance and Adjusting Plate
4 Adjusting Gas Flow Rate
4 Temperature Control
Method Development
5 Starting Guidelines
5 Thermally Labile Samples
5 Solvent Evaporation Times
5 Needle Depth
Maintenance
6 The System
6 Suggested Wash Procedure for the Needles
7 Needle Replacement
Troubleshooting
Specifications


Introduction
Introduction
Biotage®SPE Dry 96 and Biotage®SPE Dry 96 Dual from Biotage
are sample concentrator systems designed to greatly enhance
the speed of sample dry down in deep and shallow 96-well
and 384-well collection plates. The systems utilize heated gas
to accelerate the evaporation of typical lab solvents by up to
5 times or more, depending upon the application. Often this
eliminates the bottleneck associated with the concentration
step in high throughput sample screening and sample
processing using 96-well and 384-well collection plates.
The systems deliver heated gas, typically nitrogen, from
needle assemblies located on the upper and lower heads of
the systems. The needle assemblies are easily removed from
the head units for cleaning, repair, or replacement. When
concentrating samples, collection plates are placed on the
adjustable lower needle assembly and then raised to the upper
needle assembly via the sliding arm. The collection plate may be
raised to any height or distance from the upper needle assembly
allowing the user to develop methods utilizing a variety of
plates and sample volumes. Gas temperatures from both needle
assemblies are fully user-adjustable.
For operation, the systems require a gas supply and a standard
110 VAC (US and Japan) or 220 VAC (UK and Europe) power
outlet. The system’s compact size accommodates use under
any hood or micro-ventilation system.
Figure 1.The front of SPE Dry 96.
Sliding arm
and knurled
knob
Lower head
Upper needle
assembly
Upper head
Temperature
controller
Plate height
stop pin
Plate height
stops
Flow meter
Lower needle
assembly
Figure 2
Sliding arm
and knurled
knob
Lower head
Upper needle
assembly
Upper head
Temperature
controller
Plate height
stop pin
Plate height
stops
Flow meter
Lower needle
assembly
.The front of SPE Dry 96 Dual.

SPE Dry 96 and SPE Dry 96 Dual User Manual | © Biotage 2017
System Precautions and Warranty
System Precautions and Warranty
PLEASE READ ENTIRE MANUAL BEFORE OPERATING
YOUR SYSTEM
General Precautions
» The system requires a standard 110 or 220 VAC power outlet.
» The system is designed to operate with a steady
flow and pressure of compressed air or nitrogen
gas. The use of any other gas has not been tested
by the manufacturer and is not recommended.
» The system should be used under a hood, micro-ventilation,
or other appropriately ventilated location to protect
against evaporated solvent and gas accumulation.
» The maximum inlet gas pressure of the
system is 65 psi. Do not exceed 65 psi.
» The system will allow flow rates up to 60 L/min
(SPE Dry 96) or 90 L/min (SPE Dry 96 Dual). Do not
operate above the maximum flow rate as this may cause
system damage and result in injury to the operator.
» The system will operate at temperatures ranging
from ambient to 60°C at the upper head, and
ambient to 80°C at the lower head. The temperature
is determined by the operator's setting of the
digital temperature controllers. Do not attempt to
use the system outside of these guidelines.
» The system has a fully adjustable lower head that
moves vertically to accommodate most plate depths
and solvent volumes. For large volume assays where
many plates of the same size and volume will be
processed, utilize the plate height stops (located on
the left front side of the system) to avoid plate-to-plate
sample contamination and damage to the needles.
New System Inspection
When unpacking your system, inspect the system for visible
damage that may have occurred in shipping such as scratches or
dents in the casing, bent needles, damaged gauges, etc. If you
notice any damage to the system, contact Biotage immediately.
DO NOT PLUG IN OR TURN ON THE SYSTEM.
The package should include the system, a power cord, two hose
clamps, an air hose connection, and the user manual. Please
contact Biotage for immediate replacement if any of these items
are missing.
Warranty and Liability
See the “Biotage Terms & Conditions of Sale” document at
www.biotage.com.


Installation
THE SYSTEM IS USER INSTALLED
For routine operation, place the system in a well-ventilated and
easily accessible area.
Connecting the Gas Supply
The gas supply is attached to the system via a hose fitting
located on the lower rear panel of the system. Attach one end of
a suitable length of 1/4" I.D. hose to the fitting and the other end
to the pressure regulator of your gas supply. Use the two hose
clamps provided. Do not turn on the gas supply until you have
completed the set-up and have read this manual in its entirety.
The gas supply hose should be pressure rated to at least 80 psi
for safety.
Adjust the inlet gas supply to 50 psi (SPE Dry 96) or 60 psi
(SPE Dry 96 Dual).
A steady gas supply, in volume and pressure, will give you the
best results by minimizing fluctuations in temperature and
volume through the needles.
The system requires at least 25 L/min of gas flow in order to
operate the heating units. These heaters are set to shut off
automatically below 25 L/min.
While the use of an in-line filter (moisture or particle) helps avoid
sample contamination, they tend to be restrictive of the gas flow.
If you choose to use one, be sure to use high flow rate filters.
Connecting the Electrical Supply
Insert the power cord into the power inlet on the lower rear
panel of the system prior to plugging the cord into the 220 or
110 VAC power outlet. The power cord inlet also houses the
electrical breaker fuse. The system's power switch is located on
the lower left side near the rear of the SPE Dry 96 system or the
lower rear near the left side of the SPE Dry 96 Dual system; see
Figure 3 and Figure 4.
Installation
Figure 3.The rear of SPE Dry 96.
Figure 4.The rear of SPE Dry 96 Dual.
Sliding arm
and knurled
knob
Gas inlet
Fuse compartment
Power cord inlet
Power switch
Sliding arm
and knurled
knob
Gas inlet
Fuse compartment
Power cord inlet
Power switch

SPE Dry 96 and SPE Dry 96 Dual User Manual | © Biotage 2017
Operation
Once you have attached the gas supply and power cord as
described in “Installation” on page 3, the system is ready
for operation. Please take precautions when operating this
system, including the use of safety glasses, as consistent with
safe laboratory practice.
Plate Clearance and Adjusting Plate
Place the collection plate or plates (when using SPE Dry 96 Dual)
on the lower head of the system, making sure that it is/they
are properly seated in the recessed area. The needle assembly
is reversible for deep or shallow well plates. Choose the side
that best fits your plate's well height. Loosen the knurled knob,
located on the right side of the system, and raise the collection
plate or plates into the needles with the sliding arm to the
appropriate depth. Set the depth of the needles 3 to 4 mm above
the top of the sample. When the appropriate plate height is
reached, lock the plate in position by tightening the knurled knob.
For large volume assays where many plates of the same size
and volume will be processed, utilize the plate height stops
(located on the left front side of the system) to avoid plate-to-
plate sample contamination and damage to the needles. Simply
move the plate height stop pin to the location that approximates
the ideal plate height as determined above. Subsequent plates
can be raised until the stop is reached, and then locked into
position to maintain the appropriate plate height.
Figure 5.The plate height stop pin.
Adjusting Gas Flow Rate
Before applying inlet gas pressure, turn the flow meter knobs
clockwise until they stop to ensure that the flow meters are
closed. Set the pressure regulator on the inlet gas supply to
50 psi (SPE Dry 96) or 60 psi (SPE Dry 96 Dual). Slowly open the
knobs on the flow meters by turning them counterclockwise.
The flow meter bubbles will rise and then level off. The gas
flow rate is determined by placing the middle of the flow meter
bubble adjacent to the desired flow rate value. Remember to
turn on the gas flow before turning on the system power switch.
Adjust the gas flow to the desired rate for your method. A good
starting point for the flow rate of the upper needle assembly
is 50 L/min (SPE Dry 96) or 80 L/min (SPE Dry 96 Dual) and
25 L/min for the lower needle assembly. Optimum drying results
can usually be obtained by maintaining the 25 L/min flow rate
for the lower needle assembly. This allows the lower assembly
to be heated while redirecting the majority of the airflow across
the sample via the upper needle assembly.
Temperature Control
Turn the power switch on at the lower left side near the rear of
the SPE Dry 96 system or the lower rear near the left side of
the SPE Dry 96 Dual system. The temperature controllers will
illuminate, the system will perform a short self-diagnostic,
and within a few seconds the lower value will indicate the user
set point and the upper value will indicate the temperature
of the gas exiting the needles. Choose the desired operating
temperature by pressing the up or down arrows. Allow
approximately 15 minutes for the system to equilibrate before
beginning sample processing.
The small
Control indicator on the face of the temperature
controller is factory set and calibrated. The
Control indicator
shows when power is being supplied to the heating elements.
The small
Alarm indicator has been deactivated, as its function
is not required for the operation of the system. Therefore,
please disregard the
Alarm indicator.
The circular arrow keys on the temperature controllers are used
to calibrate the system and change internal electronic settings.
These keys have been deactivated on the keypad to avoid
accidental changes of these settings.
All systems are factory set and calibrated for temperature and
flow rate. If there is a suspected problem with these functions,
please contact Biotage®1-Point Support™for service.
Note: The small red indicator light on the upper right face of
the system should not be illuminated. This light indicates that
internal diagnostic systems have shutdown the system due to an
overtemp with the heaters. If this situation is encountered, turn
off the power switch and let the system cool down. Then turn the
power switch back on to reset the temperature controllers.
Operation


Method Development
Method development is application dependant and specific to
your individual needs. However, some general starting points
and guidelines may help accelerate the process. Methanol is a
typical solvent and nitrogen is the typical gas used.
Consider the following parameters when developing methods
on the system:
» Gas temperature
» System gas flow
» Composition of sample
» Volume of sample
Note: As long as the inlet gas pressure (25 to 50 psi for SPE
Dry 96 or 25 to 60 psi for SPE Dry Dual) and compressed tank
volume (>10 gal.) are sufficient, they have a negligible effect on
method development and ruggedness of the resultant method.
Starting Guidelines
A good starting point is to set:
» Inlet gas pressure at 50 psi (SPE Dry 96)
or 60 psi (SPE Dry 96 Dual).
» Flow rate of the upper needle assembly to
50 L/min (SPE Dry 96) or 80 L/min (SPE Dry 96 Dual)
and 25 L/min for the lower needle assembly.
» Upper head temperature at 50°C for aqueous/solvent
based analytes and 35°C for biological samples.
» Lower head temperature equal to 20°C higher
than the upper head temperature.
» Raise plate so that sample is ~4 mm below needles.
» Lock plate into position and note the time.
As a reference, a 500 µL sample of methanol should take about
11 minutes (SPE Dry 96) or 15 minutes (SPE Dry 96 Dual) to
reach dryness at 20% relative humidity.
Method Development
Based on your needs and sample type, you may adjust the
parameters accordingly. Since sample composition and volume
are not usually negotiable, you will probably be adjusting
temperature and system gas flow to vary your methods. If you
are working with biological samples, you may be further
restricted by the fact that too much heat may alter your sample.
Thermally Labile Samples
It is important to note that when using nitrogen, it emerges
from the needles at a lower temperature than ambient air
temperature. In fact, the higher the flow rate, the colder the
nitrogen will become. For this reason, unheated gas can be well
below the ambient temperature of the lab.
Therefore, even a 30 to 35°C temperature setting can "heat"
the gas and dramatically improve concentration times without
risking damage to thermally labile samples.
Solvent Evaporation Times
See Table 1 below.
Needle Depth
The distance from the top of the sample to the needles is only
important when establishing the evaporation point. "Following"
the sample down with the needles as it evaporates offers little
benefit in time savings.
Solution Ratio
(%)
Volume
(µL)
Flow Rate
SPE Dry/SPE Dry Dual
(L/min)
Temperature
(°C)
Evaporation Time
SPE Dry/SPE Dry Dual
(minutes)
Water 100 200 50/80 60 20/22
Water 100 500 50/50 60 44/58
Methanol 100 200 50/90 40 5/7
Methanol 100 200 50/90 55 4/5
Methanol 100 500 50/90 55 10/11
Methanol/Water 50:50 200 50/90 55 13/14
Methanol/Water 50:50 500 50/90 55 30/38
Table 1.Solvent evaporation times.

SPE Dry 96 and SPE Dry 96 Dual User Manual | © Biotage 2017
Maintenance
The System
Maintaining the cleanliness of your work area and of your
system will help ensure that your system operates efficiently.
Simply wipe down the system regularly with a clean cloth lightly
dampened with a mild solvent or cleanser.
Inspect the system regularly for worn components and replace
them immediately. The gas line and hose clamps should be
inspected prior to each use. Confirm that they are in good
condition to prevent the hose from bursting or blowing off
the system.
Removing, inspecting, and cleaning the needle assemblies
should be part of a regular maintenance program. To remove
the upper needle assembly, simply depress the latches located
on each side of the head and the needle assembly will slide
straight out for easy cleaning.
Inspect and lubricate the lower head sliding mechanism
regularly with system-grade lubricant.
Figure 6
Slide out the
needle assembly
Catch
Latch
.The upper needle assembly of the SPE Dry 96 system.
Figure 7
Slide out the
needle assembly
Latch
Catch
.The upper needle assembly of the SPE Dry 96 Dual system.
Suggested Wash Procedure for the Needles
The needles are 304 stainless steel and the head assembly is
constructed of acetal-copolymer. It is highly recommended that
an appropriate daily cleaning procedure be devised so that the
needle assemblies do not become contaminated or attacked by
harsh solvent.
There are a number of ways to clean the needle assemblies.
The two most common methods are submersion and sonication.
Submersion
1. Submerse the needles in 60% methanol/40% deionized
water for a few minutes with unheated gas flowing slowly
through the needle assembly. Agitation from the bubbles
will usually clean and lift off what may be left from the
previous sample as contamination carry-over.
2. Rinse with 100% methanol.
3. Repeat as necessary for heavily contaminated needles.
4. Remove the cleaning reagent and allow gas to flow
(unheated) until dry.
Sonication
1. Remove the head by depressing the latches located on each
side of the head; see Figure 6 and Figure 7.
2. Fill the sonicator with 60% methanol/40% deionized water.
3. Position the needle assembly in the sonicator as shown in
Figure 8.
Figure 8.The correct position of the needle assembly in the sonicator.
4. Sonicate the needle assembly for 30 minutes. This provides
the highest level of protection against sample carry-over.
5. Rinse with 100% methanol.
6. Allow head to air dry. Alternatively, it may be placed back
onto the system and dried utilizing unheated gas.
Maintenance


Maintenance
Needle Replacement
1. Remove the bad needle with long nose pliers.
2. Insert needle into the housing of the needle replacement
tool (P/N SD-9606); see Figure 9.
Figure 9.Inserting the needle into the needle replacement tool.
3. Position the needle perpendicular to the needle head
assembly; see Figure 10.
Figure 10
Pounding unit
Handle
New needle
.Inserting the new needle into the needle assembly.
4. Drive the needle into the needle assembly with the
pounding unit, until the housing of the needle replacement
tool reaches the surface of the needle assembly completely.
5. Remove the needle replacement tool

SPE Dry 96 and SPE Dry 96 Dual User Manual | © Biotage 2017
Troubleshooting
Temperature controllers do not illuminate when power switch is
turned on:
» Check electrical power connections to the system.
» Check the electrical fuse.
Temperature controllers illuminate, but the gas is not heating:
» Check to ensure that the inlet gas supply is between 25 and
50 psi (SPE Dry 96) or 25 and 60 psi (SPE Dry 96 Dual).
» Check to ensure that the system flow
rate is greater than 25 L/min.
» Check that the gas temperature is set above ambient.
Temperature controllers do not illuminate, but power switch
indicator is on:
» The heating units may be overheated. Turn off power
and let the system cool off for 10 minutes, ensure
proper airflow, then re-start. If problem continues,
please contact Biotage 1-Point Support.
No gas emits from the needles:
» Check that the inlet gas supply pressure is set between 15
and 50 psi (SPE Dyr 96) or 15 and 60 psi (SPE Dry 96 Dual).
» Check that the gas flow is set correctly.
» Ensure that the needle assembly is seated
and locked down properly.
» Check for leaks at the hose inlet and in the supply hose.
» Visually inspect needle assemblies to
ensure they are not plugged.
Certain wells do not dry down:
» Visually inspect that the needles are not blocked;
see the Maintenance section on page 6.
» Check that the needles are not bent or damaged.
» Inlet and/or operating pressure may be too low. For
more uniform drying, a higher inlet line pressure
(above 20 psi) and flow rate is optimum.
Difficult lower head movement:
» Visually inspect all of the sliding
mechanisms for obstructions.
» Ensure that the sliding guides are clean and lubricated.
» Loosen the sliding arm knurled knob 1/4 to
1/2 turn further (counterclockwise).
Difficult to reach upper head flow rate of 80 L/min (only
applicable for SPE Dry 96 Dual):
» Reduce lower head flow rate to system minimum of 25 L/min.
Troubleshooting


Specifications
Power Supply: 110 or 220 VAC, 50/60 Hz
Power Consumption: 405 W nominal
Fuse Rating: 5A fast blow
Dimensions (W X H X D): SPE Dry 96: 9.52" x 15.72" x 12.05"
(242 mm x 400 mm x 306 mm)
SPE Dry 96 Dual: 10.0" x 15.5" x 15.0"
(254 mm x 394 mm x 381 mm)
Weight: SPE Dry 96: 25 lbs (11.36 kg)
SPE Dry 96 Dual: 31 lbs (13.9 kg)
Inlet Gas Connection: 1/4" NPT
Inlet Gas Pressure: Minimum 15 psi, maximum 65 psi
Minimum Operating
Flow Rate:
25 L/min
Maximum Operating
Flow Rate:
SPE Dry 96: 60 L/min
SPE Dry 96 Dual: 90 L/min
Maximum Operating
Gas Temperature:
60°C (upper head)
80°C (lower head)
Specifications

© Biotage 2017
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