Endress+Hauser analytikjena qTOWER iris User manual

Operating Manual
qTOWER iris
Thermal Cycler

Manufacturer Analytik Jena GmbH+Co. KG
Konrad-Zuse-Straße 1
07745 Jena / Germany
Phone: [phone]
Fax: [phone]
Email: [email protected]
Technical Service Biometra GmbH
Rudolf-Wissell-Straße 30
37079 Göttingen / Germany
Phone: [phone]
Fax: + 49 551 50 68 666
Email: [email protected]
General Information http://www.analytik-jena.com
Documentation Number 10-3107-102-23
Edition A (05/2023)
Technical Documentation Analytik Jena GmbH+Co. KG
© Copyright 2023, Analytik Jena GmbH+Co. KG
For a proper and safe use of this product follow the instructions. Keep the
operating manual for future reference.

qTOWER iris Table of contents
3
Table of contents
1 Notes on this operating manual........................................................................................................................... 5
2 Intended use......................................................................................................................................................... 6
3 Security ................................................................................................................................................................. 7
3.1 Safety labeling on the device ........................................................................................................................... 7
3.2 Requirements for the operating personnel..................................................................................................... 7
3.3 Safety instructions for transport and commissioning..................................................................................... 8
3.4 Safety instructions for operation ..................................................................................................................... 8
3.5 Safety instructions for maintenance and cleaning ......................................................................................... 9
3.6 Behavior during emergencies .......................................................................................................................... 9
4 Design and function.............................................................................................................................................. 10
4.1 Design, connections and control elements ..................................................................................................... 10
4.2 Function ............................................................................................................................................................ 12
4.2.1 Fluorescence spectrometer .............................................................................................................................. 12
4.2.2 PCR thermocycler.............................................................................................................................................. 13
4.2.3 Heated lid.......................................................................................................................................................... 13
4.2.4 Plastic ................................................................................................................................................................ 14
4.2.5 Software ............................................................................................................................................................ 14
4.3 Protective devices ............................................................................................................................................. 15
4.4 Type plate.......................................................................................................................................................... 15
5 Installation............................................................................................................................................................ 16
5.1 Installation location requirements................................................................................................................... 16
5.1.1 Spatial requirements ........................................................................................................................................ 16
5.1.2 Power supply..................................................................................................................................................... 16
5.2 Installation ........................................................................................................................................................ 17
6 Operation.............................................................................................................................................................. 19
6.1 Switching the device on and off....................................................................................................................... 19
6.2 Start real-time PCR........................................................................................................................................... 20
7 Error messages ..................................................................................................................................................... 22
8 Maintenance and care.......................................................................................................................................... 23
8.1 Cleaning............................................................................................................................................................. 23
8.1.1 Cleaning the housing........................................................................................................................................ 24
8.1.2 Cleaning the sample block ............................................................................................................................... 24
8.1.3 Disinfecting the device ..................................................................................................................................... 24
8.2 Replacing the fuses........................................................................................................................................... 25
9 Transport and storage.......................................................................................................................................... 26
9.1 Transport........................................................................................................................................................... 26
9.1.1 Inserting the transport lock.............................................................................................................................. 26
9.1.2 Return................................................................................................................................................................ 27
9.1.3 Moving the device in the laboratory................................................................................................................ 28
9.2 Storage .............................................................................................................................................................. 28

Table of contents qTOWER iris
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9.3 Packing away .................................................................................................................................................... 29
10 Disposal ................................................................................................................................................................ 30
11 Specifications........................................................................................................................................................ 31
11.1 Technical data................................................................................................................................................... 31
11.2 Ambient conditions .......................................................................................................................................... 33
11.3 Standards and directives .................................................................................................................................. 34
12 Revision overview................................................................................................................................................. 35

qTOWER iris Notes on this operating manual
5
1 Notes on this operating manual
Content This user manual describes the following device models:
¡qTOWER iris96
¡qTOWER iris96UV
¡qTOWER iris96touch
¡qTOWER iris96touchUV
¡qTOWER iris384
¡qTOWER iris384UV
In this manual, these models are collectively referred to as the device. Any differences
between the models are explained in the relevant section.
The device is intended to be operated by qualified specialist personnel under observance
of the operating manual.
The operating manual provides information about the design and operation of the de-
vice and provides operating personnel with the necessary know-how for safe handling of
the device and its components. Furthermore, the operating manual includes information
on the maintenance and servicing of the device as well as information on potential
causes of malfunctions and their correction.
Conventions Instructions for actions occurring in chronological order are numbered and combined
into action units.
Warnings are indicated by a warning triangle and a signal word. The type, source and
consequences of the hazard are stated together with notes on preventing the hazard.
Elements of the control and analysis program are indicated as follows:
¡Program terms are in bold (e.g., the System menu).
¡Menu items are separated by vertical lines (e.g., System|Device).
Symbols and signal words used
in this manual
The user manual uses the following symbols and signal words to indicate hazards or in-
structions. These warnings are always placed before an action.
WARNING
Indicates a potentially hazardous situation which can cause death or very serious (possi-
bly permanent) injury.
CAUTION
Indicates a potentially hazardous situation which can cause slight or minor injuries.
NOTICE
Provides information on potential material or environmental damage.

Intended use qTOWER iris
6
2 Intended use
NOTICE
The device is intended for general laboratory use.
The device may only be used for the applications described in these operating instruc-
tions.
The manufacturer does not accept liability for any other use.
The device is a thermocycler which is licensed for real-time PCR experiments that am-
plify DNA via polymerase chain reaction (PCR) while using fluorescence spectroscopy for
the highly sensitive detection of target sequences.
The device is fully controlled from the PC or an integrated tablet using the qPCRsoft
software.
The device may only be used for the processes described in the user manual. Only the
specified use is regarded to be the intended use. Using the device for any other purpose
may compromise the safety of the user and the device.
Modifications, conversions and extensions are only permitted after consultation with
Analytik Jena. The operator alone is liable for damage caused by unauthorized modifica-
tions, conversions and extensions.

qTOWER iris Security
7
3 Security
For your own safety and to ensure error-free and safe operation of the device, please
read this chapter carefully before commissioning.
Observe all safety instructions listed in this user manual and all messages and informa-
tion displayed on the monitor by the control and analysis software.
3.1 Safety labeling on the device
Warning and mandatory action labels have been attached to the device and must always
be observed. Damaged or missing warning and mandatory action labels can cause incor-
rect actions leading to personal injury or material damage.
¡Do not remove the warning and mandatory action signs.
¡Replace damaged signs.
The following warning and mandatory action signs are used:
Warning/mandatory sign Meaning
Disconnect the power supply before opening the device
cover.
The device contains controlled substances. Analytik Jena
warrants that these substances will not be released from
the device within the next 25years provided the device is
employed as intended.
General warning sign
Warning against hot surface
3.2 Requirements for the operating personnel
The device must only be operated by qualified specialist personnel instructed in the use
of the device. The operating personnel must meet the following requirements:
¡Operate the device only after instruction and training.
¡Know and avoid dangers when working with the device.
¡Wear personal protective equipment such as protective gloves, lab coat and safety
goggles.
¡Training by Analytik Jena is recommended.
The operator of the device is responsible for compliance with safety and occupational
health regulations. The operator must meet the following requirements:
¡Provide information about national regulations on work safety and accident preven-
tion and observe them during operation of the device.
¡Instruct the operating personnel in the safe operation of the device. In doing so, also
convey the contents of the manuals for the device system.

Security qTOWER iris
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3.3 Safety instructions for transport and commissioning
Transport The device is heavy. There is a risk of injury when lifting and carrying, especially from
unsecured parts.
¡Empty the device and secure all loose parts, e.g. with adhesive tape. Close the lid.
¡Only lift the device with two people. Position each person on opposite sides of the
device and firmly grasp the underside.
¡Only transport the device in its original packaging. Insert all transport locks.
Ambient conditions during
commissioning
The device is dangerous if it is installed in an unsuitable environment.
¡Design the installation site according to the requirements in the installation plan.
¡The device must not be installed in an explosive environment.
¡Ensure that the main switch of the device is always freely accessible.
¡Keep the ventilation slits clear.
Electrical conditions The device may be dangerous if the conditions for the electrical connection are not met.
¡Only use the supplied power cable or a cable of the same size with a protective earth
conductor. Do not use an extension in the supply cable.
¡Connect the power plug to a proper power outlet to ensure that the device meets
protection class I (ground connector). Do not invalidate the protective effect by the
use of an extension line which does not have a protective conductor.
¡Check the electrical requirements of the device before connecting it to the mains.
¡Set the voltage selector switch on the device according to the mains voltage avail-
able on site.
¡Only connect the device and its system components to the power grid when they are
switched off.
3.4 Safety instructions for operation
Electrical hazard Lethal voltages may occur in the device.
¡Before each start-up, make sure that the device and its safety devices are in proper
working condition.
¡In case of malfunctions of electrical components, switch off the device immediately
and disconnect it from the electrical power supply.
¡Do not remove or bypass any protective devices such as the housing.
¡Prevent liquid from penetrating the device.
¡Do not use the device in environments with extreme humidity (>95%), or in loca-
tions in which condensation occurs.
Thermal hazard During operation, there is a risk of burns on the sample block and on the hot samples.
During rapid heating of the sample block, samples can vaporize explosively. The hot
steam can cause scalds.
¡Only operate the device if the lid is closed.
¡Only use sample vessels, microtiter plates, adhesive foils, and caps that are suitable
for PCR applications.
Mechanical hazard The device can tip over if the lid is pushed open quickly. There is a risk of crushing at the
lid.
¡Open the lid slowly without pushing it open.
¡Close the lid slowly. Make sure that fingers and hands are not trapped between the
underside and the lid.

qTOWER iris Security
9
Hazard from substances The device can be used to handle hazardous substances. The operator is responsible for
the safe handling of these substances.
¡If the device has been contaminated with hazardous substances, decontaminate it
as described in the operating manual. Use other methods only after consultation
with Analytik Jena.
¡Do not apply oil between samples and sample block. Oil can cause harmful vapors to
form.
3.5 Safety instructions for maintenance and cleaning
There is a risk of electric shock if contact is made with live components, which may lead
to serious injury.
Unauthorized servicing can lead to maladjustment or damage of the device and its sys-
tem components.
¡Work on electrical components inside the device may be carried out by customer
service only.
¡Only carry out the maintenance actions listed in the operating manual.
¡Switch off the device before maintenance and cleaning. Only work on a switched-on
device if this is expressly required by the operating manual.
¡Use only original spare parts, wear parts and consumables. These have been tested
and ensure safe operation.
¡After maintenance, ensure that all safety devices are fully functional again.
¡Clean the device with a damp, non-dripping cloth. Do not use organic solvents, abra-
sive cleaners or bleach.
3.6 Behavior during emergencies
In an emergency such as a laboratory fire, live devices put rescue personnel at risk.
¡If possible, switch off the device and its components at the power switch and discon-
nect the power cable from the mains socket.

Design and function qTOWER iris
10
4 Design and function
4.1 Design, connections and control elements
The device combines a PCR thermocycler with a patented fluorescence photometer.
The lock with handle and the LED for status display are located on the front side of the
device.
The models with integrated tablet can be controlled as stand-alone systems entirely via
the software installed on the tablet. The integrated tablet is also located on the front
side of the device.
The figure below shows the device components and the models without and with inte-
grated tablet.
1
5
4
3
6
7
2
Fig.1 Front of the devices, left without and right with integrated tablet
1 Upper part with fluorescence spectrom-
eter
2 Operating tablet
(only for models with integrated tablet)
3 USB port
(only for models with integrated tablet)
4 Lock with handle
5 LED for status display 6 Lower part with thermocycler
7 Device name
The device is opened by folding back the upper part and the cover for the sample block
and fluorescence photometer it contains. To this end press the handle in until the lock
disengages with a click and the upper part gently snaps open. The upper part can then
be folded back from the handle.

qTOWER iris Design and function
11
1
2
3
Fig.2 Open device
1 Heated lid 2 Sample block
3 Locking pins
The mains connection and the power switch are located on the device rear.
There are also connection interfaces are located on the rear of the device: a USB port for
connection to the PC and an Ethernet port for connection to a network.
1
3
4
8910
7
2
5
6
Fig.3 Rear of the device
1 Ventilation for fluorescence spectrome-
ter
2 USB port for connection cable to an ex-
ternal PC
3 Ethernet port (only for models with in-
tegrated tablet)
4 Ventilation grille on the thermal cycler
5 Service connection 6 Ethernet port for network connection
7 Type plate 8 Power connection
9 Fuse holder for device fuses Mains switch

Design and function qTOWER iris
12
The voltage selector switch is located on the bottom of the device, behind a cover. It can
be used to adjust the operating voltage to the mains voltage.
Fig.4 Underside of the device with voltage selector switch
The following accessories are included in the delivery scope of the device:
¡Power cable
¡Connection cable for connecting to the PC
¡CD or USB stick with qPCRsoft software, with manual for software and device
¡Operating manual and software manual (print version)
¡Packaging and packaging instructions
Fig.5 Mains and connection cable
Only use the mains cable supplied or a mains cable with the same specifications.
4.2 Function
4.2.1 Fluorescence spectrometer
The detector unit for a thermal block with 96 wells is a patented 8-channel epi-fluores-
cence photometer with fiber multiplexer and a mechanical scanning device.
The detector unit for a thermal block with 384 wells is a patented 16-channel epi-fluo-
rescence photometer with fiber multiplexer and a mechanical scanning device.
The device is an open platform for real-time PCR and supports both intercalating dyes as
well as individual sensors and kits from various manufacturers. The device can be used
in various applications, such as expression analyses, genotyping and the detection of
pathogens.
Light source A long-life, sturdy five-color LED (blue, green, white, red, and UV) in the device is used
as an excitation light source for the emission of fluorescent dyes. The light source does
not require any preheating time.

qTOWER iris Design and function
13
Multiplexer with color modules The color modules with the excitation filters are mounted on a rotating filter wheel, the
multiplexer. Analytik Jena offers a number of color modules of which up to six can be in-
stalled simultaneously in the multiplexer.
Retrofit or replacement of the installed modules may be carried out by the Analytik Jena
service team at any time.
NOTICE
If you intend to extend the application spectrum of the device with regard to using filter
modules, always contact the service department because extensions– to be performed
by the operator himself– are not intended by the manufacturer.
Measuring head The measuring head as a mechanical scanning unit scans the sample block column by
column.
Detector The detector (photomultiplier) allows measurement of the sample fluorescence in up to
six spectral channels during PCR, providing verification of multiple target sequences in a
single PCR reaction. The signal from the fluorescent dyes excited by a light source corre-
lates quantitatively with the amount of PCR product and may be shown in real-time.
4.2.2 PCR thermocycler
The thermal block with 96 wells is made of silver for the best possible performance and
thermal conductivity. The silver is coated with gold for corrosion protection. Due to its
excellent heat conductivity, silver equilibrates extremely quickly, providing maximum
speed and uniform temperature distribution.
This achieves a high temperature homogeneity and uniformity in combination with
heating rates of up to 8°C/s and cooling rates of up to 5.5°C/s.
The thermal block with 384 wells is made of aluminum that conducts heat very well.
This achieves a high temperature homogeneity and uniformity in combination with
heating rates of up to 4°C/s and cooling rates of up to 2°C/s.
This powerful thermal block is suitable for high-throughput applications in particular.
The gradient function of the thermal block is particularly suited for establishing new pri-
mary pairs.
The thermal blocks are perfectly sealed to prevent condensed water from penetrating
the Peltier elements underneath the sample block and other parts of the electronics.
This protects the Peltier elements and prolongs the service life of the device.
4.2.3 Heated lid
The device comes equipped with an automated heated lid. This can be set to
30to110°C and prevents condensation forming in the area of the reaction tubes above
the block surface level. Furthermore, the heated lid guarantees – regardless of the con-
sumables used – a reliable contact between the reaction tubes and the thermal block
during the entire real-time PCR run thanks to a constant contact pressure. This signifi-
cantly improves temperature uniformity.

Design and function qTOWER iris
14
4.2.4 Plastic
NOTICE
Unsuitable sample vessels and microtiter plates can lead to instrument damage and in-
correct results.
¡Only use sample vessels and microtiter plates that are suitable for a PCR application.
¡Seal the samples. Use the sample vessels with lids and adhesive foil for microtiter
plates.
¡The optical transparency of the foils affects the fluorescence signal directly. Use only
clear adhesive foils and optical lids as offered for real-time PCR.
Sample blocks in 96-well SBS format are suitable for use with 0.2mL individual tubes,
8-well strips and 96-well microtiter plates.
Sample block in 384-well SBS format are only suitable for use with standard PCR plates
with 384 wells.
These and other consumables can be ordered from Analytik Jena.
Regardless of the sealing method used, thanks to the lid technology, the same pressure
is always applied to the consumables for absolutely reproducible conditions.
The device is not limited to specific detection reagents or the plastic products of a spe-
cific manufacturer.
4.2.5 Software
The device can be controlled via the qPCRsoft software on an external PC or an inte-
grated tablet.
Desktop software The desktop software provides the following functions:
¡Device control and monitoring
¡Design of real-time PCR experiments and their evaluation
¡Storage of methods (templates) and measuring results (projects)
¡User management
¡Planning and evaluation of
– Absolute quantifications
– Relative quantifications
– ΔΔCt analyses
– DNA melting curves
– Genotyping
– End point analyses
¡Results export to MS EXCEL or as CSV file
¡Results printout
¡Results export to extended programs for the analysis of real-time PCR data (e.g.
GenEx)

qTOWER iris Design and function
15
Software installed on the inte-
grated tablet
The software installed on the integrated tablet offers the following functions:
¡Device control and monitoring
¡Design of real-time PCR experiments and their evaluation
¡Monitoring the measurement
¡Calculating the Ct value
¡Storage of methods (templates) and measuring results (projects)
¡Results export to MS EXCEL or as CSV file
¡Results export to extended programs for the analysis of real-time PCR data (e.g.
GenEx, qBASE)
¡Data transfer to the desktop PC software via USB stick
A detailed description of the software can be found in the software manual.
4.3 Protective devices
The device is equipped with protective devices. The protective devices ensure safe oper-
ation of the device and must not be bypassed. The following table lists the available pro-
tective devices and their functions.
Protective device Function
Housing Enclosure of the electrical and optical components.
Enclosure of surfaces that become hot during measure-
ment.
Locking pins Locking of the lid opening while a measurement is un-
derway.
Stray light detection Termination of the heating process in case of stray light
detection due to opening of the lid.
4.4 Type plate
The type plate is located on the rear of the device. It provides the following information
shown in the figure:
¡Manufacturer and address
¡Protection class of the housing
¡Safety symbols (Caution: Observe the accompanying documents!
¡Device number
¡Year of manufacture
¡Disposal instructions (Do not dispose of as domestic waste!)
¡Conformity and test sign
¡Electrical connection data
¡Serial number
¡Order number
¡Device type and model

Installation qTOWER iris
16
5 Installation
5.1 Installation location requirements
Ambient conditions The climate conditions for the installation location are listed in the technical specifica-
tions (→ "Ambient conditions" 33). If required, ensure that the room is tempera-
ture-controlled.
Installation location require-
ments
¡This laboratory device is designed for indoor use.
¡Do not use the device in wet and damp environments. Keep the device surface clean
and dry.
¡Avoid direct sunlight and radiation from heaters onto the device. If necessary, pro-
vide air conditioning.
¡Place the device on a heat-resistant and acid-resistant surface.
¡Do not locate the device near sources of electromagnetic interference.
¡Avoid mechanical shocks and vibrations.
¡Do not use the device in explosion-hazard environments.
¡Place the device on a stable surface.
¡The installation site must be free of drafts, dust and caustic fumes.
¡Keep the ventilation slits free and do not obstruct them with other devices.
5.1.1 Spatial requirements
NOTICE
When opening the device, the upper device hood tilts back. Provide adequate space for
this.
The opened device has a spatial requirement of 61.3cmx30.4cmx47.7cm
(HxWxD). In addition, keep a safety distance of at least 10cm to other equipment or
walls.
Additional space is required next to the device for the PC, monitor and possibly a printer.
The PC, monitor and printer may also be placed on a separate table.
PC, monitor and printer are optional if you control the device via an integrated tablet.
5.1.2 Power supply
WARNING
Risk of electric shock!
The device may only be connected to a properly earthed power outlet in accordance with
the voltage specifications on the type plate.
The device operates on single-phase alternating current. Prior to making the connection
check that the voltage selection switch of the device is set to the correct value.
The device may only be used with the supplied power cable or a power cable with the
same specification (1.5m length, shielded, with grounding conductor).

qTOWER iris Installation
17
Electrical connection require-
ments Operating voltage 100/115/230V(AC)±10%
Line frequency 50/60Hz
Power consumption ≤850W
Device fuse 2x10AT/250V
5.2 Installation
WARNING
Risk of electric shock!
Check that the mains connection conditions match those indicated on the type plate on
the rear of the device.
Before connecting the device to the mains network set the correct operating voltage at
the voltage switch on the underside of the device.
NOTICE
Unsuitable packaging material may cause damage to the device! Keep the original pack-
aging and transport lock for subsequent transports.
The following steps are required during the installation of the device:
¡Checking the set operating voltage and correcting it if it does not match the voltage
supplied at the site
¡Connect the device to the PC and install the software on the PC. This step is optional
for models with an integrated tablet.
¡Connecting the device to power
Proceed as follows for the installation:
}Remove the device, the connection cables and the operating manual from the trans-
port packaging. For models without an integrated tablet, also remove the installation
CD or USB stick from the transport packaging.
}Wait until the device has reached room temperature for commissioning.
}Verify that the delivery is complete. Check the device and accessories for transport
damage.
In the event of an incomplete delivery or transport damage, please contact Analytik
Jena.
Fig.6 Voltage selection switch on the bottom of the device

Installation qTOWER iris
18
}Set the operating voltage:
– Place the device on its side. Remove the cover from the voltage selection switch
on the bottom.
– Adjust the switch using a screwdriver or a coin so that the arrow points in the di-
rection of the mains voltage available on site.
– Replace the cover on the selection switch and put the device back on its feet.
Fig.7 Rotating feet on the bottom of the device
}If the surface where the device is being installed is not level: The device has ad-
justable feet that can be unscrewed. Unscrew the adjustable feet so that the device is
horizontal.
}Open the lid of the device. To do so, press in the red handle on the front until the
lock clicks open. Fold back the upper part of the device.
}Remove the transport lock and put it aside for later transports.
}Connect the connection cable to the device interface and to the PC. This step is op-
tional for models with an integrated tablet.
}Make sure that the power switch of the device is switched off. Connect the power ca-
ble to the device. Insert the plug of the power cable into the power socket.
üThe device is ready for commissioning.
NOTICE!Ensure that the power switch and the power cable can be easily accessed.
This is important in the event that the device needs to be disconnected from the supply
voltage.
Control via external PC }Switch on the PC. Install the software on the PC. Observe the information in the soft-
ware manual for this.
}Switch on the device via the mains switch.
When first switched on, the device is detected as a connected device. After the driv-
ers install automatically, the device is ready for operation. If the drivers are not in-
stalled automatically, you can complete the installation via the Windows routine.
The drivers can be found on the installation CD or USB stick.
}Start the software.
üThe device is ready for operation.
Control via integrated tablet }Switch on the device via the mains switch.
üThe software starts automatically.
üThe device is ready for operation.

qTOWER iris Operation
19
6 Operation
6.1 Switching the device on and off
NOTICE
Remove the transport lock before commissioning. Keep this for subsequent transports.
}Switch on the device via the mains switch on the rear of the device.
üThe device is initialized. During this time, the status LED on the front side of the
device flashes. The device is ready for operation when the status LED is lit contin-
uously in green.
For models with an integrated tablet, the software starts automatically. The device is
ready for operation when the status LED is lit continuously.
When controlling via an external PC, start the software as follows:
ðThe PC is switched on.
ðThe device is switched on and the status LED on the front of the device is continually
green.
}Start the software.
}In the "Device selection" window, select the desired device for control.
üThe software establishes the connection to the device.
üThe device is connected and can be controlled via the software.
Status LED The status LED is located on the front of the device next to the device name. It displays
the operating state of the device:
¡During the device initialization the LED flashes red/green.
¡As soon as the device is ready for operation, the LED switches to solid green.
¡During a measurement, the LED flashes red/green.
¡In case of a device error, the LED will turn red.
Switching the device off
NOTICE
Do not switch off the device during a PCR run!
Keep the device closed even if switched off to prevent the sample block from becoming
contaminated. Dust or other contamination can affect the fluorescence measurements.
Once the PCR run is finished, the software can be exited and the device switched off by
pressing the power switch.
Close the software for models with integrated tablet via the button on the start
screen and shut down the tablet.
Then switch off the device by pressing the power switch.

Operation qTOWER iris
20
6.2 Start real-time PCR
WARNING
Biological hazard!
Exercise caution when working with potentially infectious materials. Wear suitable pro-
tective equipment, e.g. protective gloves.
WARNING
Risk of eye injury!
The rapid heating of the thermal block can result in the explosive vaporization of liquids
when the thermal block is opened during the PCR run. Always wear safety goggles dur-
ing operation.
CAUTION
Hot surfaces
The thermal block, the samples, and the heated lid reach high temperatures. There is a
risk of burns during contact.
Sample blocks in 96-well SBS format are suitable for use with 0.2mL individual tubes,
8-well strips and 96-well micro titer plates.
Sample block in 384-well SBS format are only suitable for use with standard PCR plates
with 384 wells.
These and other consumables can be ordered from Analytik Jena.
The recommended plastic products from other manufacturers are listed in the corre-
sponding section of this operating manual.
Measurement of fewer samples
for devices with 96-capacity
sample block
NOTICE!The force of the lid contact pressure was designed for a fully populated
block. If only a few samples are used in the block, ensure that the lid contact pressure is
distributed symmetrically. Otherwise the sample vessels or the heated lid may become
damaged from excess contact pressure.
When inserting only a few sample, proceed as follows:
¡When using few individual tubes, also insert additional (empty) tubes of the same
height in the four corner positions of the block.
Fig.8 Positions for additional individual tubes when measuring with few samples