Optika B-510 Series User manual

Ver. 2.2 2019
INSTRUCTION MANUAL
B-510 Series
Model
B-510BF
B-510ERGO
B-510ASB
B-510PH
B-510FL
B-510LD1
B-510LD2
B-510-2
B-510-2F
B-510-3
B-510-5

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Table of Contents
1. Warning 4
2. Symbols and conventions 4
3. Safety Information 4
4. Intended use 4
5. Overview 5
5.1 B-510BF / B-510ERGO 5
5.2 B-510PH 7
5.3 B-510ASB 9
5.4 B-510-2 / B-510-2F / B-510-3 / B-510-5 10
5.5 B-510FL 11
5.6 B-510LD1 / B-510LD2 12
6. Unpacking 13
7. Assembling 13
7.1 B-510BF / B-510ERGO 13
7.2 B-510PH 14
7.3 B-510ASB 15
7.4 B-510-2 / B-510-2F / B-510-3 / B-510-5 16
7.5 B-510FL 17
7.6 B-510LD1 / B-510LD2 18
7.7 Microscope assembling 19
7.7.1 B-510BF / ERGO / PH / ASB 19
7.7.2 B-510-2 / B-510-2F / B-510-3 / B-510-5 20
7.7.3 B-510FL 23
7.7.4 B-510LD1 / B-510LD2 25
7.8 Polarizing set (optional) 26
8. Brighteldobservationprocedures(B-510BF / B-510ERGO) 27
9. Use of the microscope (B-510BF / B-510ERGO / B-510-2-2F-3-5) 28
9.1 Light intensity adjustment 28
9.2 Coarse focus tension adjustment 28
9.3 Coarse upper limit lever 28
9.4 Stage 29
9.5 Dioptric adjustment 29
9.6 Adjusting the interpupillary distance 29
9.7 Use of eye shields 30
9.8 Centering the condenser 30
9.9 Aperture diaphragm 31
9.10 Use of oil immersion objective 31
9.11 Use of the pointer (B-510-2/2F/3/5) 32
9.12 Use of the polarizer (optional) 32
10. CondenserforBrighteld/Darkeld/PhaseContrast(B-510PH) 33
10.1 BrighteldObservation(BF) 33
10.2 DarkeldObservation(DF) 33
10.3 Phase Contrast Observation (PH) 34
10.4 Useofthegreenlter 35
11. CondenserforBrighteld/PhaseContrast(B-510ASB) 35
11.1 BrighteldObservation(BF) 36
11.2 Phase Contrast Observation (PH) 36
12. Fluorescence observation procedures (B-510FL) 37
13. Fluorescence observation procedures (B-510LD1 / LD2) 37
14. Use of the microscope (B-510FL /B-510LD1 / B-510LD2) 38
14.1 Microscope setting (B-510FL) 38
14.2 Use of the microscope (B-510FL) 40
14.2.1 Use of the shutter 40
14.2.2 Use of light excluding plate 41
14.3 Use of the microscope (B-510LD1 / LD2) 41
15. Simultaneous observation in Phase Contrast + Fluorescence (B-510FL) 42
16. Microphotography 43
16.1 Use of C-mount cameras 43
16.2 Useofreexcameras 43

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17. Maintenance 44
18. Troubleshooting 45
Equipment disposal 47

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-
ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-
lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to
use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe
operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

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5. Overview
PHOTO/TV PORT
DIOPTER
ADJUSTMENT
RING
NOSEPIECE
COARSE
UPPER
LIMIT LEVER
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS ADJUSTMENT
KNOB
OBSERVATION
HEAD
CONDENSER HEIGHT
ADJUSTMENT KNOB
OBSERVATION
ERGO HEAD
5.1 B-510BF / B-510ERGO

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EYEPIECES
STAGE
CONDENSER
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION ADJUSTMENT RING
SLIDE HOLDER
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
B-510BF / B-510ERGO (opposite side)

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PHOTO/TV PORT
DIOPTER
ADJUSTMENT RING
NOSEPIECE
FINE FOCUS
KNOB
COARSE
UPPER
LIMIT LEVER
COARSE FOCUS
KNOB
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS
ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
5.2 B-510PH

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EYEPIECES
STAGE
CONDENSER
BINOCULAR
TUBE
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
TENSION ADJUSTMENT RING
SLIDE HOLDER
PHASE RINGS
CENTERING
SCREWS
B-510PH (opposite side)

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5.3 B-510ASB
PHOTO/TV PORT DIOPTER
ADJUSTMENT RING
NOSEPIECE
FINE FOCUS
KNOB
COARSE
UPPER
LIMIT LEVER
COARSE FOCUS
KNOB
OBJECTIVES
APERTURE
DIAPHRAGM
FIELD
DIAPHRAGM
MAIN SWITCH /
BRIGHTNESS
ADJUSTMENT KNOB
OBSERVATION
HEAD
CONDENSER
HEIGHT
ADJUSTMENT
KNOB

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5.4 B-510-2 / B-510-2F / B-510-3 / B-510-5
SIDE
OBSERVATION
HEADS
POINTER COLOR
SELECTOR
POINTER BRIGHTNESS
ADJUSTMENT / POINTER
MOVEMENT
SIDE
OBSERVATION
HEADS

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5.5 B-510FL
The main microscope commands remain unchanged: only the uorescence parts are highlighted.
LIGHT PATH
SELECTOR
FLUORESCENCE CUBE
SELECTOR
HBO FLUORESCENCE
LAMP HOUSING
FLUORESCENCE
POWER SUPPLY

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FLUORESCENCE
CUBE SELECTOR
LED FLUORESCENCE
HOUSING
5.6 B-510LD1 / B-510LD2
The main microscope commands remain unchanged: only the uorescence parts are highlighted.
BRIGHTNESS
ADJUSTMENT
KNOB

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6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-510BF / B-510ERGO
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Immersion oil
⑥ Allen wrench
⑦ Tension adjustment tool
⑧ Dust cover
⑨ Power supply
③
⑤
⑥
⑦
⑧
⑨
①
②
④

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① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Centering telescope
⑥ Immersion oil
⑦ Allen wrench
⑧ Tension adjustment tool
⑨ Dust cover
⑩ Green lter
⑪ Power supply
①
②
③④
⑤
⑥
⑦
⑧
⑨
⑩
⑪
7.2 B-510PH

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7.3 B-510ASB
① Microscope frame
② Eyepieces
10x (one pair)
12,5x (one pair)
③ Objectives
④ Observation head
⑤ Centering telescope
⑥ Immersion oil
⑦ Allen wrench
⑧ Tension adjustment tool
⑨ Dust cover
⑩ Green lter
⑪ Power supply
①
②
③④
⑤
⑥
⑦
⑧
⑨
⑩
⑪

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7.4 B-510-2 / B-510-2F / B-510-3 / B-510-5
①
②
③
④
⑤
⑥
⑦
⑧
⑩
⑨
① Microscope frame
② Eyepieces
10x/22 (one pair for main head)
10x/20 (one pair for B-510-2 / 2F)
10x/20 (two pairs for B-510-3)
10x/20 (four pairs for B-510-5)
③ Objectives
④ Main observation head
⑤ Side observation heads
one for B-510-2 / 2F
two for B-510-3
four for B-510-5
⑥ Immersion oil
⑦ Allen wrench
⑧ Tension adjustment tool
⑨ Dust cover
⑩ Power supply
②

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⑪⑫
① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Lamp housing
⑥ Epi-illuminator
⑦ HBO bulbs
⑧ Allen wrench
⑨ Tension adjustment tool
⑩ Dust cover
⑪ UV protection shield
⑫ Power supply
⑬ Power cord
⑭ Fluorescence power supply
⑮ Light excluding plate
①
②
③
④
⑤
⑥
⑦
⑧
⑨
⑩
7.5 B-510FL
⑬
⑭
⑮

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① Microscope frame
② Eyepieces
③ Objectives
④ Observation head
⑤ Epi-illuminator
⑥ Allen wrench
⑦ Tension adjustment tool
⑧ Immersion oil
⑨ Dust cover
⑩ Power supply
⑪ Light excluding plate
①
②
③
④
⑤
⑥
⑦
⑧
⑨
⑩⑪
7.6 B-510LD1 / B-510LD2

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Fig. 1
Fig. 2
Fig. 4
Fig. 3
7.7 Microscope assembling
7.7.1 B-510BF / ERGO / PH / ASB
1. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Hold the head with one hand during the
locking in order to avoid that the head falls.
2. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
3. Condenser is pre-installed in the factory. To
remove the condenser use an Allen wrench
1,5mm diam and operate on the locking screw
placed on the right side of the condenser holder.
4. Screw each objective into the thread of
the nosepiece, clockwise with increasing
magnication. (Fig. 3)
5. Insert the power supply jack in the connector
placed at the rear side of the microscope. (Fig. 4)

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7.7.2 B-510-2 / B-510-2F / B-510-3 / B-510-5
1. Place the splitter attachment of the multidiscussion
system and tighten the lock screw ① on the right
side of the frame. (Fig. 5)
2. Connect the 5Vdc power supply to the rear socket
of the splitter attachment. (Fig. 6).
3. Connect the rst part of the extension tube to the
optical splitter. Insert the tube into the splitter all
the way down and screw on the black sealing ring
completely. (Fig. 7-8).
• Every connection is labelled with a letter
printed on both sides of the connection. Make
sure to match the letters in order to correctly
assemble the microscope.
Fig. 5
Fig. 6
Fig. 8
Fig. 7
①