Hanil Cellspin II User manual

Cellspin II
Clinical Centrifuge
Date of Purchase
Serial No.
Place of purchase
User Manual

Copyright ⓒ2020 Hanil Scientific Inc. All rights reserved.
Contact Us
The contents in this operating manual are subject to change for device improvement.
Cellspin II
If you haveand questions,contactHanilScientificInc.or placeof purchase.
[phone]
Order, Inquiry:[e-mail]
Tech. support:[e-mail]
Clinical Centrifuge
UM-Cellspin II(E)(Rev.1), 20201229

CONTENTS
1. General Considerations-------------------------------------------------
1.1 Safety -3
1.2 Transport & Storage -4
1.3 Safety label -4
3. Installation ----------------------------------------------------------------
3.1 Packing Inspection -7
3.2 Installation -7
3.2.1 Selecting the location -7
4. Operation ----------------------------------------------------------------
4.1 Control panel -9
4.2 Opening and closing the lid -10
4.3 Loading and removal of the rotor -10
4.4 Loading cytocell accessories -11
4.5 Setting RPM/RCF -12
4.6 Setting run time -12
1.4 Electric safety information -4
5. Maintenance------------------------------------------------------------
6. Troubleshooting---------------------------------------------------------
6.1 General errors -15
7. Rotor ----------------------------------------------------------------------
3
5
7
9
3.2.2 Power connetion -8
4.8 Saving / Recall a program -12
4.9 Start / Stop the centrifugation run -12
14
5.1 Care instructions -14
5.2 Cleaning -14
15
16
4.7 Setting deceleration rate -12
2. Productdescription-----------------------------------------------------
2.1Appearance -5
2.2Components -5
2.3Technicalspecifications-6

1. General Considerations
1.1 Safety
3 l www.ihanil.com
Safety precautions are to prevent personal injury, product damage, and
breakdown from possible dangers during use.
Please observe all safety measures described in this manual.
1. Thecentrifugeshouldbe installedon flat surface to maintainlevel.
2. Checkthevoltage to be used,beforeconnectingthecentrifugeto thepowersource.
3. Onlyuserotors,parts,andaccessories providedby HanilScientificInc.
HanilScientificInc.is notresponsiblefordamages of thedeviceand accidentscausedby
usingpartsandaccessoriesnot recommended.
4. Do not exceedthemaximumratedspeedof therotor or bucketsin use.
5. Makesureto preparenecessarysafetymeasuresbeforeusingsamplesthataretoxic or
radioactivesamplesor pathogenicor samplesor infectiousblood.
6. Substancesthatmaygeneratevolatileor explosivevaporcannot be centrifuged.
7. If thecentrifugeis contaminated by toxic or radioactivesamplesor infectiousbloodsamples,
removecontaminants completelyandtakeneedfulactions suchas ventilationor isolation
of centrifuge.
8. Thebalancing workof samplesshoulddone in advancebeforeoperation.
9. Beforeoperation, rotor andchambershouldbe dry.
10. Do not attempt to slowor stopthe spinningrotor by hand.
11. Onlycentrifugewithrotor androtor lid firmly locked.
12. Do not blockvents.
13. When serving thecentrifuge,be sureto removecontaminantsin advance.
14. Pleasecontact theplace of purchaseor HanilScientificInc.forproductrepairs.
15.When operating according to the IEC61010-2-020 standard, the safety distance (30 cm)
around the centrifuge must be observed to preserve ventilation for smooth instrument
operation and the safety of users and the surrounding environment.
16. Turnthepowerswitchoffafterusingthedevice.
17. Disconnect thepowerplugbeforecleaningor leftunusedforalongperiod of time.

1. General Considerations
1.2 Transport & Storage
• The device and the accessories
may only be stored in dry rooms
• Only lift and transport the
device with sufficient number of
helpers.
-Storage-
Ambient temperature 5℃~35℃
Maximum relative humidity 30%~85%
Air pressure 500~1060hpa
-Transport-
Ambient temperature -10℃~40℃
Maximum relative humidity 10%~90%
Air pressure 500~1060hpa
1.3 Safety label attached to a product
Mark indicatingdanger
and warning.
Mark indicatinga place in
danger of electric shock.
1.4 Electric safety information
[email protected]om / [e-mail] l 4
1.Useapowercordonlyprovidedwithequipment.
2.Usesocketswithaprotectiveearthconductorandsuitablepowercord.
3.Donot usean extensioncord.
4.Donot placeanythingon thepowercable
5.Donot block air vents.
6.Ifyouhavethefollowingemergencies,shutoffthepowersupplyandunplug
thepowercordfromoutletandcontact yourplace of purchase.
-Unusual noisesor smellfromtheequipment.
-Damageor wearof apowercord.
-Breakdownof circuitbreaker,fuse or safetydevice.
-If youspillliquidon theequipment.
-If theequipmenthasbeendamaged.
Caution label when inserting
rotor / tube and lid closure.

2. Product description
5 l www.ihanil.com
①Control panel
②Lid window
③Centrifuge Lid
④Power switch
①
③
④
②
2.2Components
2.1 Appearance
①Cellspin II
②Rotor:A-cyto-12
③User manual
④Emergency Door Tool

2. Product description
2.3 Technical Specifications
[e-mail] / [e-mail] l 6
Max. RPM 3,000 rpm
Max. RCF 1,175 xg
Max. Capacity 12 slides x 0.5~0.6 mL
Time control < 100 min or continuous
Program memory 10
Dimension (W x D x H, mm) 318 x 436 x 260
Weight without rotor 23kg
Power requirement (VA) 556 VA
Power input (V, Hz) 220V, 50/60Hz
Cat. No. CE-II

3. Installation
3.1 Packing Inspection
•Check packing conditions carefully, before unpacking.
•Contact Hanil Scientific Inc. immediately if damages found.
•Check the delivery for completeness.
•You can get contact details on packing boxes and the back of the manual.
3.2 Installation
3.2.1 Selecting the location
Installation on hard and flat ground.
• Centrifuge should be installed on hard and flat place.
• If the centrifugeis installed in an inclined place, the shaft may be bent due to the
weight of the rotor.
Goodventailation.
• For air circulation and safety, maintain a 30cm“clearance envelope”around the
centrifuge while the rotor is spinning.
Constant temperature/humidity
• Centrifuge equipped with the sensitive electronic software which is fragile with
humidity and temperature.
•Must avoid direct ray or heater and be put in the ambience of controlled temp.and air.
Avoid the corrosive gas
• Install the centrifuge in a place where corrosive gas is not generated.
• Sulfur dioxide gas and chlorine gas may cause corrosion.
Leveling
• The shaft should be put exactly vertical on the hotizontaliy flat ground by
the leveling tool
7 l www.ihanil.com

3. Installation
3.1 Packing Inspection
3.2.2 Power Connection
• Connect the device to voltage sources which correspond to the electrical
requirements on the label attached to the device.
• Use sockets with a protective earth conductor and provided powercord.
1. Plug the cord into the outlet.
2. Switch the centrifugeon using the power switch.
[e-mail] / [e-mail] l 8

4. Operation
4.1 Control Panel
Button Function
RPM
RCF To set the speed and for automatic conversion between RPM and RCF
Time To set time up to 99 min 59sec (0:00:00: continuous)
Soft Start
Stop To operate with slower and smoother acceleration / deceleration
Open To open the instrument lid
Start
Stop To start or stop operation
Prog To save the set values and recall the saved programs
9 l www.ihanil.com
①
②
③
④
Lamp Function
①/ ②①‘RPM’ lamp: The light indicates that you can set the rpm.
②‘RCF’ lamp: The light indicates that you can set the rcf.
③/ ④
③‘Minute’ lamp: The light indicates that you can set the run time in
minutes.
④‘Second’ lamp: The light indicates that you can set the run time in
seconds.

4. Operation
4.2 Opening the lid
• The lid can only be opened when the centrifuge is switched on.
• Do not reach with your fingers between the housing and lid.
• Close the lid completely before operation.
1. To open the door, press the [Open] button.
The door is automatically opened with end alarm upon completion of the rotation.
Close the door until you hear the door clank.
When the door is opened, the door LED turns on
4.3 Loading and removal of the rotor
•Only use rotors, parts, and accessoriesprovided by Hanil Scientific Inc.
•Do not use scatchedor cracked rotor in use.
Loading
①Clean the motor shaft and the chamber.
②Load the rotor vertically onto the motor shaft.
Unloading
①Hold the blue handle at the center of the rotor and lift it up.
[e-mail] / [e-mail] l 10

4. Operation
4.4 Loading Cytocell accessories
• Only use tubes and accessories provided or approved by Hanil Scientific Inc.
• Always use the same type of tube.
• Tubes should be loaded symmetrically.
• Do not exceed the maximum rated speed of the tube.
• Same volume of sample should be put on tubes.
• Check symmetric loading by balncing tubes with scales.
①Check the maximum load for each tube.
②Put cytocell accessories into rotor holes vertically.
③Cytocell accessorieslocated opposite each other must be the same type and contain
the same quantity.
11 l www.ihanil.com
-Cytocell accessories assembly-
①Place the rubber plate and filter on the sus holder in this order.
-Loading cytocell accessories-

4. Operation
4.5 Setting RPM/RCF
4.6 Setting run time
[Setting RPM]
1. Press a [RPM/RCF]button once.
RPM MODE is on by pressing the [RPM/RCF] button once.
2. Press the [▲▼] buttons to change the set value.
Speed setting unit: 10 rpm
3. Press the [RPM/RCF]button again to set the value.
[Setting RCF]
1. Press a [RPM/RCF]button twice.
RCF MODE is on by pressing the [RPM/RCF]button twice.
2. Press the [▲▼] buttons to change input value
3. Press the [RPM/RCF]button again for saving.
1. Press the [TIME] button once.
The MIN mode is on by pressing the [TIME] button once.
2. Press the [▲▼] buttons to change the set value.
3. Press the [TIME] button again to set the value.
[e-mail] / [e-mail] l 12
[Setting the MIN Value]
[Setting the SEC value]
1. Press the [TIME] button twice.
The SEC mode is on by pressing the [TIME] button twice.
2. Press the [▲▼] buttons to change the set value.
3. Press the [TIME] buttonagain to set the value.

4. Operation
4.7 Save / Recall a program
[Save a program]
1. Set parameters.
2. Keep the [PROG] button pressed over 3 seconds to save your set values.
The LED of [PROG]button and SEC/Save are turned on.
3. Input the program number by using the [▲▼] button.
Up to 10 programs can be stored.
4. Press the [PROG] button again to complete saving.
The set value is saved.
In case of no input for 5 seconds, you will get out of the Save mode.
[Recall a program]
1. To recall a saved program, press the [PROG]button shortly.
The LED of [PROG]and MIN/Call are turned on.
2. Enter the program number you want to recall by pressing [▲▼] button.
3. Press [PROG]button once again.
The set values of the saved program are displayed.
In case of no input for 5 seconds, you will get out of the Recall mode.
13 l www.ihanil.com
4.8 Start / Stop the centrifugation run
1. After setting all parameters, press [START/STOP]button.
While running, the ‘START LED’ is turned on.
To stop the operation, press the [START/STOP]button while running.

4. Operation
4.9 Soft start / stop
The [SOFT START/STOP]button is used for gentle acceleration and deceleration for
sensitive samples.
1. After setting TIME and RPM, press the [SOFT START/STOP]button once.
The LED of [SOFT START/STOP]button is on while running.
The door is automatically opened when the operation is completed.
When operating in the [SOFT] mode, the operation cannot be stopped by pressing the
[START/STOP] button.
[e-mail] / [e-mail] l 14

5. Maintenance
5.1 Care instructions
• The following procedures should be performed regulary.
①Regularly inspect the rotor chamber for check the motor shaft is nomal.
②Rotate the shaft with your hand to make sure it turns smoothly
③Use the stopwatchto check that the time setting is correct
5.2 Cleaning
• Before cleaning the centrifuge, be sure to switch off the device and
disconnect the power cord.
15 l www.ihanil.com
•Oustsideof thedevice
①Cleantheoutsideof equipment witha softanddrycloth.
②Do not useaggressive chemicals on thedevice suchas Alchol, Benzene, Acetone
or Phenol.
③Usethe stopwatchto check thatthetimesettingis correct
▶If thedevice is contaminated,use a mildcleaningfluid to clean.
④Make suredo not scratchthe surface of equipmentwhen cleaningit.
▶Do not use ametalsponge.
▶If thedevice is rusted,removeit withamilddetergentandwipe it withadrycloth.
•Chamber
①If therotor chamberis not dry,wipemoisturefromthechamberwitha drycloth.
②If therotor chamberis dirty, clean thechamber and remove moisture withadry
cloth.
•Rotor
①To preventcorrosion,remove therotor fromtherotor chamberafter finishing
centrifusion.
②If anysampleis spiltinsidetherotor,washanddrytherotor well.
•Disposal
①In caseof productis to be disposed of,thelocalwasteslaws and regulationsare
to be observed.

6. Troubleshooting
6.1 General errors
Problem Recommended Action
Power failure Check the power cord connection.
Check the power fuse of the device.
Device cannot be started Check the lid is closed completely.
Lid cannot be opened Press the ‘Lid open button’.
Lid cannot be closed
Unusual noise and vibration
[e-mail] / [e-mail] l 16
Check if thedevice is installed inclined
Clean and thedoor latchand
close thelid.
Checkif thelid latchis damaged.
Reloadtherotor symmetrically.
Reloadthetubessymmetrically.
Reload therotor

6. Troubleshooting
6.2 Error messages
Error Possible Causes Actions
Error 1 RPM
* If the speed does not reach 200 rpm within 2 seconds
after motor starts to operate, this message may appear.
* Check whether the motor is normally working or not.
* If the error message does not disappear, please contact
a Service Engineer of your local Hanil Scientific’s partner.
Error 2 Lid Open
Error 3 Motor Overheating
a Service Engineer of your local Hanil Scientific’s partner.
Error 4 Low Voltage
Error 5 High Voltage
Error 6 Overspeed
* If the instrument spins faster than allowed (1,000 rpm
higher than the set speed), it may cause overload to
motor capacity or any trouble in the output of motor.
* Turn off and on the power supply to check up the
instrument.
* If the error message does not disappear, please contact
a Service Engineer of your local Hanil Scientific’s partner..
17 l www.ihanil.com
*If thedooropenswhilespinningor hasanytroublein
thedoorsensor,thismessagemaycomeup.
* Clean thedoor latchandclose thedoor completely.
Checkthedoorclosing statuson thedisplaywindow.
*If theerrormessagedoesnotdisappear,pleasecontact
aServiceEngineerof yourlocalHanilScientific’spartner.
*If themotoris overheated,thismessagemaycomeup.
* Remove thepowersupplyforan hour,andturnon the
powerto checkup theinstrument.
*If theerrormessagedoesnotdisappear,pleasecontact
*If thepowerinput(V/Hz)is 10% lower thanthe
recommendedpower,thismessagemaycomeup.
* Turnoffthepowersupplyandcheckthevoltageof the
Powersupply(V/Hz).
*Use AVRto provideproperpower.
*If thepowerinput(V/Hz)is at 10% higherthanthe
recommended,thismessagemaycomeup.
*Turnoffthepowersupplyandcheckthevoltageof the
Powersupply(V/Hz).
*Use AVRto provideproperpower.

6. Troubleshooting
6.2 Error messages
Error 7 Software
* Contact a Service Engineer of your local Hanil Scientific’s partner and
get the firmware upgrade. Wire disconnection or tuning of the
instrument must be performed only by a Service Engineer authorized
by Hanil Scientific’s partner.
Error 8 Imbalance
* Check the balance status of the samples in the rotor and turn off
and on the instrument to check the status.
* If the error message does not disappear, please contact a Service
Engineer of your local Hanil Scientific’s partner.
Error 9 RPM Sensor
Engineer of your local Hanil Scientific’s partner.
[e-mail] / [e-mail] l 18
Error
Action
Possible Causes
*If theinstalledsoftwarehave anybugs,thismessagemaycomeup.
*If therotorrecognition fails,thismessage comesup.
* Themessagewill be clearedby couplingan appropriaterotor
*Disassembleandcoupleacompatiblerotorand restart
theinstrumentto checkoutthestatus.
*If theerrormessagedoesnotdisappear,pleasecontact a Service

6. Troubleshooting
6.3 Emergency Lid Open
19 l www.ihanil.com
If thedevicedoor cannotbe opened,youcanactivtetheemergencyopen manually.
1. Waitforrotor to stopbeforeactivatingtheemergencyopen.
2. Insertthe pin intotheemergencyopen holeandpushit untilthedooris opened.
Emergency Door Open Hole